LOCUS       Exported     3163 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3163
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1201..1248
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3096..3112
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3113..3144
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3162
                     /label="mnaA"
                     /note="Catalyzes the conversion of UDP-N-acetylglucosamine into UDP-N-acetylmannosamine, a precursor of the teichoic acid linkage unit."
                     /translation="MKKLKVMTVFGTRPEAIKMAPLVLELKKYPEIDSYVTVTAQHRQMLDQVLDAFHIKPDFDLNIMKERQTLAEITSNALVRLDELFKDIKPDIVLVHGDTTTTFAGSLAAFYHQIAVGHVEAGLRTGNKYSPFPEELNRQMTGAIADLHFAPTGQAKDNLLKENKKADSIFVTGNTAIDALNTTVRDGYSHPVLDQVGEDKMILLTAHRRENLGEPMENMFKAIRRIVGEFEDVQVVYPVHLNPVVREAAHKHFGDSDRVHLIEPLEVIDFHNFAAKSHFILTDSGGVQEEAPSLGKPVLVLRDTTERPEGVEAGTLKLAGTDEENIYQLAKQLLTDPDEYKKMSQASNPYGDGEASRRIVEELLFHYGYRKEQPDSFTGK"
     primer_bind     complement(1253..1269)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1336..1924)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2115..2975)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2976..3080)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG AAAAAGCTGA AAGTCATGAC GGTTTTTGGT ACTCGTCCGG AAGCAATCAA
       61 AATGGCACCT TTGGTGCTGG AACTGAAAAA ATATCCAGAA ATCGATTCCT ATGTTACCGT
      121 GACTGCTCAG CACCGCCAAA TGCTGGATCA GGTTCTGGAT GCGTTCCACA TTAAACCGGA
      181 TTTTGATTTG AATATCATGA AAGAGCGCCA GACCTTGGCG GAGATTACGA GCAATGCATT
      241 GGTCCGTCTG GATGAACTGT TTAAAGACAT TAAACCGGAC ATTGTCCTGG TTCACGGCGA
      301 TACTACCACC ACTTTCGCCG GTTCTCTGGC CGCGTTCTAC CATCAAATTG CGGTTGGCCA
      361 CGTCGAGGCA GGCCTGCGCA CTGGTAACAA GTACAGCCCA TTTCCGGAGG AGTTGAATCG
      421 TCAGATGACG GGTGCGATCG CAGATCTGCA TTTCGCACCG ACCGGCCAAG CGAAGGATAA
      481 TCTGCTGAAA GAAAATAAAA AAGCGGACAG CATCTTTGTT ACCGGTAACA CCGCAATTGA
      541 CGCGCTGAAT ACGACGGTTC GTGATGGCTA TAGCCACCCG GTTCTGGACC AGGTGGGCGA
      601 GGACAAAATG ATCCTCCTTA CCGCGCACCG TCGCGAAAAC CTGGGTGAGC CGATGGAAAA
      661 TATGTTCAAA GCAATCCGTC GTATCGTCGG TGAATTCGAG GACGTTCAGG TCGTGTATCC
      721 GGTCCACCTG AACCCGGTTG TTCGTGAGGC AGCGCATAAA CACTTTGGTG ACAGCGATCG
      781 TGTACATCTG ATCGAGCCGC TGGAGGTTAT CGACTTTCAT AACTTTGCTG CAAAATCTCA
      841 CTTCATCCTG ACGGACAGCG GTGGCGTTCA GGAAGAGGCG CCGAGCCTGG GTAAACCGGT
      901 TCTGGTTCTG CGTGACACCA CTGAGCGCCC AGAAGGCGTC GAGGCCGGTA CGTTGAAACT
      961 GGCGGGCACG GATGAAGAAA ACATCTACCA GTTGGCGAAG CAGTTACTGA CGGATCCGGA
     1021 TGAGTATAAG AAAATGAGCC AGGCGAGCAA CCCGTACGGT GATGGCGAAG CTAGCCGTCG
     1081 CATTGTGGAG GAGCTTCTGT TCCATTATGG TTATCGTAAA GAACAGCCGG ACAGCTTCAC
     1141 GGGTAAGTGA AGAGCTTAGA GACCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1201 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1261 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1321 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1381 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1441 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1501 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1561 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1621 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1681 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1741 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1801 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1861 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1921 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     1981 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     2041 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     2101 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     2161 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     2221 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     2281 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     2341 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     2401 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     2461 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     2521 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     2581 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     2641 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     2701 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     2761 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     2821 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     2881 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     2941 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     3001 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     3061 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     3121 GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGG GAG
//