LOCUS       Exported     3607 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3607
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..3593
                     /label="tagH"
                     /note="Part of the ABC transporter complex TagGH involved in teichoic acids export. Responsible for energy coupling to the transport system."
                     /translation="MKLKVSFRNVSKQYHLYKKQSDKIKGLFFPAKDNGFFAVRNVSFDVYEGETIGFVGINGSGKSTMSNLLAKIIPPTSGEIEMNGQPSLIAIAAGLNNQLTGRDNVRLKCLMMGLTNKEIDDMYDSIVEFAEIGDFINQPVKNYSSGMKSRLGFAISVHIDPDILIIDEALSVGDQTFYQKCVDRINEFKKQGKTIFFVSHSIGQIEKMCDRVAWMHYGELRMFDETKTVVKEYKAFIDWFNKLSKKEKETYKKEQTEERKKEDPEAFARFRKKKKKPKSLANAIQIAILSILTVFMAGTMFFNAPLRTIASFGAIPQNEVKNHHGDAKGKSEERLTAINKQGFIANEKAAAYKDQGLKQKADVTLPFGTKVTVAAKGKQAAKIKFDGHSYYVKQSAVATNMKHAELHATAFTSYVSQNAASSYEYFLKFLGDSSTSIQSKLNGYTEGNKADGRKTLNFDYEKISYVLENDKATELIFHNISPINPASLSLSDSDVLYDSSKKRFLVNTDDQVFAVDNEEHTLTLMLK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAACTGAA GGTGAGCTTT CGTAACGTAA
     2041 GCAAACAGTA CCATCTGTAT AAAAAACAAA GCGATAAAAT TAAAGGTTTG TTCTTCCCGG
     2101 CGAAAGACAA CGGTTTCTTC GCTGTTCGTA ACGTGAGCTT CGACGTCTAC GAGGGTGAGA
     2161 CTATCGGTTT TGTTGGCATT AACGGTAGCG GCAAATCTAC GATGAGCAAT CTGTTGGCAA
     2221 AAATCATTCC GCCGACCAGC GGTGAGATCG AGATGAACGG TCAGCCGAGC TTGATTGCGA
     2281 TCGCGGCGGG CCTGAATAAC CAGCTGACGG GCCGTGATAA CGTGCGTTTG AAGTGCCTGA
     2341 TGATGGGTCT GACCAACAAG GAAATTGACG ACATGTATGA CAGCATCGTT GAATTTGCGG
     2401 AAATTGGCGA CTTCATCAAT CAACCGGTCA AAAATTATTC GAGCGGTATG AAAAGCCGTC
     2461 TGGGTTTTGC CATCAGCGTT CATATTGACC CGGATATCCT GATCATTGAT GAAGCGCTGA
     2521 GCGTTGGTGA CCAGACGTTT TACCAAAAGT GTGTAGATCG TATCAACGAG TTTAAAAAGC
     2581 AGGGCAAGAC CATCTTCTTT GTGAGCCACT CCATCGGTCA GATCGAAAAG ATGTGTGACC
     2641 GTGTCGCCTG GATGCATTAT GGTGAGCTGC GTATGTTTGA CGAAACTAAG ACTGTCGTTA
     2701 AAGAGTATAA GGCATTTATT GATTGGTTTA ATAAGCTGTC CAAGAAAGAG AAAGAAACCT
     2761 ATAAGAAGGA GCAGACCGAG GAACGCAAGA AAGAGGATCC GGAAGCCTTC GCCCGCTTCC
     2821 GTAAGAAGAA AAAGAAGCCT AAAAGCTTAG CAAATGCGAT CCAAATTGCA ATTTTGAGCA
     2881 TTCTGACCGT TTTCATGGCC GGCACGATGT TTTTTAATGC GCCGCTGCGT ACTATCGCCA
     2941 GCTTCGGCGC AATCCCGCAA AATGAAGTGA AGAACCACCA CGGTGACGCA AAGGGCAAAT
     3001 CCGAAGAACG TTTGACCGCA ATCAATAAAC AGGGTTTCAT TGCAAATGAA AAGGCGGCTG
     3061 CCTACAAAGA CCAAGGTCTG AAACAGAAAG CGGACGTGAC GCTGCCGTTT GGCACGAAGG
     3121 TTACTGTGGC CGCGAAAGGC AAACAGGCGG CAAAAATTAA ATTCGATGGC CATAGCTACT
     3181 ACGTTAAACA GAGCGCAGTG GCAACTAACA TGAAACATGC GGAACTGCAC GCGACGGCGT
     3241 TTACCTCATA CGTGTCCCAG AATGCTGCGA GCAGCTACGA ATATTTCCTG AAATTTCTGG
     3301 GTGACAGCAG CACGAGCATT CAAAGCAAGC TGAACGGTTA CACGGAAGGT AACAAGGCAG
     3361 ACGGTCGCAA AACGCTGAAC TTCGATTACG AAAAAATCAG CTATGTGCTG GAAAATGATA
     3421 AAGCGACGGA ACTGATCTTC CACAATATTT CCCCGATCAA CCCGGCGAGC TTGAGCCTGA
     3481 GCGACTCGGA TGTCCTGTAT GATTCCAGCA AAAAACGTTT TCTGGTGAAT ACCGACGACC
     3541 AGGTTTTTGC GGTGGATAAC GAAGAACATA CCCTGACCCT GATGTTGAAG TGAAGAGCTT
     3601 AGAGACC
//