LOCUS       Exported     4261 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4261
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2299..2346
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4194..4210
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4211..4242
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..4260
                     /label="tagF"
                     /note="Responsible for the polymerization of the main chain of the major teichoic acid by sequential transfer of glycerol phosphate units from CDP-glycerol to the disaccharide linkage unit. Synthesizes polymers of approximately 35 glycerol phosphate units in length."
                     /translation="MSLVVDTNKRKQKGKSFYTEEQKKVMIENTVIKCILKSLKNNLGSLELLISIDSEHQFLEDYQLFLKLKERRSGTESEFPLQNTGSLEYKTEINAHVLPMPVEMGQTYDFYVEFRKKYEDAEQEPLLKRLSAEVNSIERAFHVDQTTELLILPYTTDKGNFSIKVKREAKIIRFDQIEISSEEISITGYAGYLSSENQYRIKNLNLILKKGGETPIEEKFPIKLERKTHGLENMRADGFVPELYDFEVKVPLKEIPFSNEKRYVYRLFMEYICNDDEGTDIQFNSTALVLGDRKNKLKGLVSIIKTNNAPVRYEVFKKKKKQTLGIRVNDYSLKTRMKYFIKGKKKRLVSKIKKITKMRNKLITKTYKSLFMMASRMPVKRKTVIFESFNGKQYSCNPRAIYEYMRENHPEYKMYWSVNKQYSAPFDEKGIPYINRLSLKWLFAMARAEYWVVNSRLPLWIPKPSHTTYLQTWHGTPLKRLAMDMEEVHMPGTNTKKYKRNFIKEASNWDYLISPNGYSTEIFTRAFQFNKTMIESGYPRNDFLHNDNNEETISLIKSRLNIPRDKKVILYAPTWRDDQFYAKGRYKFDLDLDLHQLRQELGNEYIVILRMHYLVAENFDLGPFEGFAYDFSAYEDIRELYMVSDLLITDYSSVFFDFANLKRPMLFFVPDIETYRDKLRGFYFDFEKEAPGPLVKTTEETIEAIKQISSPDYKLPVSFGPFYDKFCYLESGRSSEKVVNTVFKAE"
     primer_bind     complement(2351..2367)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2434..3022)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3213..4073)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4074..4178)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 GTCTCAAATG AGCCTGGTCG TGGACACGAA CAAACGTAAG CAGAAAGGTA AAAGCTTTTA
       61 TACCGAGGAA CAAAAGAAGG TCATGATCGA AAACACCGTT ATCAAGTGTA TTTTGAAATC
      121 CCTGAAGAAC AATCTGGGTA GCCTGGAGTT GCTGATTAGC ATTGACAGCG AGCATCAGTT
      181 CTTGGAGGAC TACCAGCTGT TTCTGAAGCT GAAGGAACGT CGTTCCGGTA CGGAAAGCGA
      241 ATTCCCGTTG CAAAATACGG GTAGCCTGGA ATATAAAACC GAGATCAACG CTCATGTTCT
      301 GCCTATGCCG GTTGAAATGG GTCAGACCTA CGATTTTTAT GTTGAGTTCC GTAAAAAATA
      361 CGAGGACGCC GAGCAGGAGC CACTGTTGAA ACGCTTGAGC GCGGAGGTGA ATAGCATCGA
      421 ACGTGCGTTT CACGTCGATC AAACGACCGA GCTGCTGATC CTGCCGTATA CCACCGATAA
      481 GGGTAACTTT AGCATTAAGG TTAAGCGTGA GGCGAAGATT ATCCGTTTTG ACCAGATTGA
      541 GATCAGCAGC GAGGAAATTT CTATCACCGG TTACGCGGGT TATCTGTCTA GCGAAAACCA
      601 GTACCGTATT AAAAATCTGA ATCTGATCCT GAAGAAAGGT GGCGAAACGC CGATCGAGGA
      661 AAAATTTCCG ATCAAGCTGG AAAGAAAAAC GCATGGTCTG GAGAACATGC GCGCTGATGG
      721 TTTCGTTCCG GAATTGTACG ACTTTGAAGT CAAGGTGCCG CTGAAGGAGA TTCCTTTCAG
      781 CAACGAGAAG CGTTACGTGT ACCGTCTGTT CATGGAGTAC ATCTGTAACG ACGATGAAGG
      841 TACGGACATC CAATTTAATA GCACGGCGCT GGTCCTGGGC GATCGTAAGA ATAAACTGAA
      901 AGGTCTGGTC AGCATCATTA AGACGAACAA CGCCCCGGTC CGCTACGAAG TTTTCAAAAA
      961 GAAGAAAAAA CAAACCCTGG GCATCCGTGT GAATGATTAC TCTCTCAAGA CGCGCATGAA
     1021 ATACTTCATT AAAGGCAAGA AGAAGCGTCT GGTTTCTAAG ATTAAAAAGA TCACGAAAAT
     1081 GCGTAACAAG TTAATCACCA AGACTTATAA GAGCCTGTTC ATGATGGCGA GCCGCATGCC
     1141 GGTGAAACGC AAAACGGTTA TTTTCGAGAG CTTTAATGGT AAACAGTATA GCTGTAATCC
     1201 GCGTGCGATT TATGAATACA TGCGTGAGAA TCACCCGGAG TATAAGATGT ATTGGAGCGT
     1261 GAATAAACAA TATAGCGCGC CGTTCGACGA AAAAGGTATC CCGTATATCA ATCGCCTGAG
     1321 CCTGAAATGG CTGTTCGCTA TGGCAAGAGC AGAGTACTGG GTGGTGAACT CTCGTTTGCC
     1381 GCTGTGGATT CCAAAACCGA GCCATACCAC CTATCTGCAA ACCTGGCATG GCACCCCGCT
     1441 GAAACGCCTG GCAATGGACA TGGAAGAGGT TCACATGCCA GGTACCAATA CGAAGAAGTA
     1501 CAAACGTAAT TTCATTAAAG AAGCTAGCAA TTGGGATTAC CTGATCAGCC CGAACGGTTA
     1561 CAGCACGGAG ATCTTTACGC GTGCCTTTCA GTTCAACAAG ACTATGATTG AGAGCGGCTA
     1621 TCCGCGCAAC GACTTCCTGC ATAATGATAA CAACGAAGAA ACCATTAGCC TGATCAAGAG
     1681 CCGTCTGAAT ATTCCGCGTG ACAAGAAGGT GATCCTGTAT GCACCTACCT GGCGTGATGA
     1741 CCAGTTCTAC GCAAAGGGTC GCTACAAATT CGACCTGGAT TTAGACTTGC ACCAACTGCG
     1801 TCAGGAATTG GGCAATGAGT ACATTGTCAT TCTGCGCATG CATTACTTAG TGGCAGAAAA
     1861 TTTTGATCTG GGCCCGTTCG AGGGTTTTGC CTATGATTTC AGCGCATATG AAGATATTCG
     1921 CGAACTGTAC ATGGTCAGCG ATCTGCTGAT CACCGACTAC AGCAGCGTGT TCTTCGACTT
     1981 TGCGAATCTC AAACGTCCGA TGCTGTTTTT CGTTCCGGAT ATTGAAACCT ATCGTGATAA
     2041 GCTGCGTGGT TTCTACTTCG ACTTTGAAAA AGAAGCGCCT GGTCCGCTGG TCAAGACGAC
     2101 CGAGGAAACC ATCGAAGCAA TTAAGCAAAT CTCTAGCCCG GACTATAAGT TGCCTGTCAG
     2161 CTTCGGTCCG TTCTACGATA AGTTTTGCTA CCTGGAAAGC GGTCGCTCCA GCGAGAAAGT
     2221 TGTCAACACC GTTTTCAAAG CGGAGTGAAG AGCTTAGAGA CCGCTGAGGT GTCAATCGTC
     2281 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     2341 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     2401 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     2461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     2521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     2581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     2641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     2701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     2761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     2821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     3001 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     3061 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     3121 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     3181 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     3241 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     3301 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     3361 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     3421 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     3481 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     3541 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     3601 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     3661 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     3721 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     3781 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3841 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3901 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3961 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     4021 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     4081 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     4141 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     4201 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     4261 G
//