LOCUS       Exported     3169 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3169
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3155
                     /label="tagB"
                     /note="Catalyzes the addition of a single glycerol phosphate residue to the prenoldiphosphate-linked disaccharide, as a primer for polymerisation by TagF."
                     /translation="MKIRSLLANCYLYFLSAIAFFLQWVKPESKVTLLISFEANAKAILEEYEQGQYSYKLNILYTQQASAIAESFPNVDAYLLQEKNPIHLIKAVYLMFNSKVIITDNYFLLTSVLNKRKQTKCIQVWHANGSLKKFGLEDITNMQRTKTDIKRFQKVYSSYDYLTVGSEEMANIFKKSFGIKDNQLLKIGVPLTDPYYRENKKKISDTLNIQRKKIILYAPTFRDYNMQSIQLPFTEEQLIHQLKEEYVLFVKLHPAIQNNIDIKYSSDYIKDVSNYALFDLLMAADILITDYSSVPFEFSILNKPILFYTYDLKLYQQKRGLVDNYLSIIPGRACYDSESLINEIQTPFNYSKIKVFSDRWNKYSDGNSSQNLLNFIENLIS"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGATTCG TAGCTTGCTG GCCAATTGTT
     2041 ATCTGTATTT TCTGAGCGCG ATCGCCTTTT TTCTGCAATG GGTGAAACCG GAAAGCAAGG
     2101 TGACGCTGCT GATTTCCTTT GAAGCGAATG CCAAGGCTAT CCTGGAAGAG TATGAACAAG
     2161 GTCAATACTC GTACAAGCTG AACATCTTAT ATACCCAACA GGCCAGCGCG ATTGCAGAGA
     2221 GCTTCCCGAA TGTGGACGCG TATCTGCTGC AGGAAAAGAA TCCGATCCAT CTGATCAAAG
     2281 CAGTCTATCT GATGTTCAAC AGCAAAGTGA TCATTACCGA TAACTACTTC CTGCTGACCA
     2341 GCGTCCTGAA CAAGCGTAAA CAGACCAAGT GCATCCAGGT TTGGCATGCG AATGGCAGCT
     2401 TGAAGAAATT TGGCTTGGAG GACATTACCA ACATGCAACG CACCAAAACG GACATTAAGC
     2461 GCTTCCAAAA GGTTTACAGC TCCTATGACT ACCTGACGGT GGGCAGCGAA GAGATGGCGA
     2521 ACATTTTTAA GAAATCGTTT GGTATCAAGG ACAACCAGCT GCTGAAGATC GGTGTGCCGC
     2581 TGACCGACCC GTACTACAGA GAAAACAAGA AGAAGATCTC CGACACCCTG AATATTCAGC
     2641 GCAAGAAAAT CATCCTGTAT GCTCCGACCT TCCGCGACTA TAACATGCAA AGCATCCAAC
     2701 TGCCGTTTAC GGAGGAGCAA CTGATCCACC AGCTGAAGGA GGAGTATGTT CTGTTTGTTA
     2761 AGCTGCACCC GGCCATTCAG AATAATATTG ACATTAAGTA TAGCTCGGAC TACATTAAAG
     2821 ACGTCAGCAA CTATGCGCTG TTCGACCTGT TAATGGCGGC GGACATTCTG ATCACCGACT
     2881 ATAGCAGCGT CCCGTTTGAG TTCAGCATCC TGAATAAGCC GATTCTGTTC TACACGTATG
     2941 ACCTGAAATT GTACCAACAA AAACGTGGTT TGGTCGATAA CTATCTGAGC ATCATTCCGG
     3001 GCCGTGCGTG CTACGACAGC GAAAGCCTGA TTAACGAGAT CCAGACCCCG TTCAATTATA
     3061 GCAAAATCAA GGTGTTCAGC GACCGTTGGA ATAAGTATTC GGATGGCAAC AGCAGCCAAA
     3121 ATCTGCTGAA CTTCATCGAA AATCTGATCT CCTGAAGAGC TTAGAGACC
//