LOCUS Exported 3334 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3334 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..3320 /label="murAA" /note="Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. Essential for cell growth." /translation="MEKIIVRGGQKLNGTVKVEGAKNAVLPVIAASLLASEEKSVICDVPTLSDVYTINEVLRHLGADVHFENNEVTVNASYALQTEAPFEYVRKMRASVLVMGPLLARTGHARVALPGGCAIGSRPIDQHLKGFEAMGAEIKVGNGFIEAEVKGRLQGAKIYLDFPSVGATENLIMAAALAEGTTTLENVAKEPEIVDLANYINGMGGKIRGAGTGTIKIEGVEKLHGVKHHIIPDRIEAGTFMVAAAITEGNVLVKGAVPEHLTSLIAKMEEMGVTIKDEGEGLRVIGPKELKPIDIKTMPHPGFPTDMQSQMMALLLRASGTSMITETVFENRFMHAEEFRRMNGDIKIEGRSVIINGPVQLQGAEVAATDLRAGAALILAGLVAEGHTRVTELKHLDRGYVDFHQKLAALGADIERVNDESASEQENKEVVSDLNA" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGAAAAAAT TATCGTCCGC GGTGGTCAGA 2041 AGCTGAATGG TACCGTTAAA GTGGAAGGTG CGAAGAATGC AGTCCTGCCG GTTATTGCGG 2101 CTAGCTTGCT GGCATCCGAA GAGAAATCGG TCATTTGTGA CGTGCCGACC CTGAGCGACG 2161 TGTACACTAT CAACGAGGTT CTGCGCCACC TGGGCGCGGA TGTCCACTTC GAAAATAATG 2221 AGGTGACGGT CAACGCTTCC TACGCGCTGC AGACCGAGGC GCCGTTCGAG TACGTCCGTA 2281 AAATGCGCGC CTCCGTTCTG GTCATGGGTC CGCTGCTCGC TCGTACGGGC CACGCACGTG 2341 TCGCACTGCC GGGTGGCTGC GCGATTGGCA GCCGTCCTAT CGATCAACAC CTGAAGGGTT 2401 TTGAGGCCAT GGGCGCAGAG ATTAAAGTGG GTAATGGTTT TATCGAAGCG GAAGTTAAGG 2461 GTCGTCTGCA AGGTGCTAAA ATCTACCTGG ACTTCCCGAG CGTGGGCGCT ACCGAAAACT 2521 TGATCATGGC CGCCGCGCTG GCAGAAGGCA CCACCACCCT GGAGAACGTT GCTAAAGAGC 2581 CGGAAATTGT GGACCTGGCA AACTATATCA ATGGCATGGG TGGCAAAATC CGTGGTGCGG 2641 GTACCGGCAC CATTAAGATC GAGGGTGTCG AGAAACTGCA CGGCGTTAAG CACCATATTA 2701 TTCCTGACCG CATTGAAGCA GGCACGTTTA TGGTGGCCGC GGCGATTACC GAAGGTAATG 2761 TTCTGGTTAA AGGTGCCGTC CCGGAACACC TGACCTCCCT GATTGCGAAA ATGGAAGAAA 2821 TGGGCGTCAC GATCAAGGAT GAAGGTGAGG GCCTGCGTGT GATCGGTCCG AAGGAACTGA 2881 AGCCGATTGA CATCAAAACT ATGCCGCATC CAGGCTTCCC GACGGACATG CAAAGCCAAA 2941 TGATGGCCCT GCTCTTGCGT GCGTCTGGCA CCAGCATGAT TACTGAAACG GTTTTCGAGA 3001 ATAGATTCAT GCACGCGGAG GAATTTCGTC GCATGAATGG TGATATTAAA ATCGAGGGCC 3061 GCAGCGTCAT TATCAACGGC CCAGTCCAGC TGCAGGGTGC CGAGGTCGCG GCGACCGATC 3121 TGCGCGCTGG TGCCGCTCTG ATTCTGGCCG GTCTGGTCGC GGAGGGTCAT ACCCGTGTTA 3181 CTGAACTGAA ACACCTGGAT CGTGGTTATG TGGATTTTCA CCAGAAGTTG GCGGCGCTGG 3241 GTGCAGATAT CGAGCGTGTG AATGATGAAA GCGCAAGCGA GCAAGAAAAC AAAGAGGTGG 3301 TCAGCGACCT GAATGCCTGA AGAGCTTAGA GACC //