LOCUS       Exported     3631 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3631
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3617
                     /label="pyrG"
                     /note="Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates."
                     /translation="MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITNELKDRVYRAGKETNADVVITEIGGTVGDIESLPFLEAIRQMKSDIGRENVMYIHCTLVPYIKAAGELKTKPTQHSVKELRSLGIQPNIIVVRTEMPISQDMKDKIALFCDIDTKAVIECEDADNLYSIPLELQKQGLDKLVCEHMKLACKEAEMSEWKELVNKVSNLSQTITIGLVGKYVELPDAYISVVESLRHAGYAFDTDVKVKWINAEEVTENNIAELTSGTDGIIVPGGFGDRGVEGKIVATKYARENNIPFLGICLGMQVASIEYARNVLGLKGAHSAEIDPSTQYPIIDLLPEQKDVEDLGGTLRLGLYPCKLEEGTKAFEVYQDEVVYERHRHRYEFNNEFRQQMEEQGFVFSGTSPDGRLVEIIELKDHPWFVASQFHPEFKSRPTRPQPLFKGFIGASVEAANQK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGACCAAATA CATCTTTGTC ACGGGTGGCG
     2041 TGGTGTCATC CCTGGGCAAA GGCATCGTGG CCGCCTCACT GGGTCGTCTG CTGAAGAACC
     2101 GTGGTCTGAA CGTGACCATT CAAAAGTTCG ACCCGTACAT TAATGTGGAT CCGGGCACCA
     2161 TGAGCCCGTA CCAACATGGT GAAGTGTTCG TGACCGATGA TGGTGCCGAA ACGGATCTGG
     2221 ACTTGGGCCA CTATGAACGT TTTATCGATA TCAACCTGAA TAAGTTTAGC AATGTGACGA
     2281 CTGGCAAAAT TTACAGCACT GTTCTGAAAA AGGAACGCCG TGGCGATTAC CTGGGCGGCA
     2341 CCGTTCAAGT CATCCCGCAC ATCACCAATG AACTGAAGGA TCGCGTGTAC CGTGCCGGTA
     2401 AAGAAACCAA TGCGGACGTG GTGATTACCG AGATCGGTGG CACGGTGGGT GACATCGAAT
     2461 CCCTGCCGTT CCTGGAGGCT ATTCGTCAAA TGAAGTCGGA CATCGGTCGC GAAAATGTGA
     2521 TGTATATTCA TTGTACCCTG GTCCCTTACA TTAAGGCTGC TGGCGAGCTG AAAACTAAGC
     2581 CTACCCAGCA TTCCGTGAAG GAGTTGCGTA GCCTGGGTAT TCAGCCGAAT ATTATCGTGG
     2641 TGCGCACCGA AATGCCGATC AGCCAGGACA TGAAGGATAA AATCGCGCTG TTCTGTGATA
     2701 TTGACACGAA GGCAGTTATT GAGTGCGAAG ATGCGGACAA CCTGTACTCC ATTCCACTCG
     2761 AGTTGCAGAA GCAGGGCCTG GATAAACTGG TGTGCGAGCA CATGAAACTG GCTTGTAAGG
     2821 AAGCTGAGAT GAGCGAGTGG AAAGAGCTGG TTAACAAAGT TAGCAACTTG TCCCAGACTA
     2881 TCACCATTGG TTTGGTGGGC AAATATGTTG AGCTGCCGGA CGCGTATATC TCGGTGGTGG
     2941 AAAGCCTGCG TCATGCCGGT TATGCCTTCG ATACCGATGT GAAAGTTAAA TGGATCAACG
     3001 CAGAGGAGGT TACCGAGAAC AATATCGCCG AGCTGACCAG CGGTACCGAT GGCATTATCG
     3061 TTCCGGGTGG CTTCGGTGAC CGCGGTGTTG AAGGTAAAAT CGTTGCAACC AAGTATGCGC
     3121 GTGAGAACAA TATTCCGTTC TTGGGCATCT GTCTGGGTAT GCAAGTGGCA AGCATCGAAT
     3181 ATGCGCGCAA TGTGCTGGGT CTGAAAGGCG CGCACTCCGC GGAAATTGAT CCGTCCACTC
     3241 AGTACCCGAT CATCGATCTG CTGCCGGAGC AGAAGGATGT CGAGGACCTG GGTGGCACCC
     3301 TGCGTCTGGG CCTGTACCCG TGCAAACTGG AGGAAGGCAC CAAGGCATTC GAGGTTTACC
     3361 AGGACGAGGT CGTTTACGAA CGTCACCGCC ACCGTTATGA ATTTAACAAC GAGTTCCGTC
     3421 AACAGATGGA GGAACAGGGT TTCGTGTTTT CCGGTACGTC CCCGGATGGT CGTCTGGTTG
     3481 AAATCATTGA ACTGAAAGAT CACCCGTGGT TCGTCGCAAG CCAGTTCCAT CCGGAGTTCA
     3541 AGAGCCGCCC AACCCGCCCA CAGCCGTTGT TCAAGGGCTT CATTGGTGCC TCCGTTGAAG
     3601 CGGCAAATCA GAAGTGAAGA GCTTAGAGAC C
//