LOCUS       Exported     3859 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3859
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3845
                     /label="walK"
                     /note="Member of the two-component regulatory system WalK/WalR involved in the regulation of the ftsAZ operon, the yocH and ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Phosphorylates WalR."
                     /translation="MNKVGFFRSIQFKITLIYVLLIIIAMQIIGVYFVNQVEKSLISSYEQSLNQRIDNLSYYIEQEYKSDNDSTVIKDDVSRILNDFTKSDEVREISFVDKSYEVVGSSKPYGEEVAGKQTTDLIFKRIFSTKQSYLRKYYDPKSKIRVLISAKPVMTENQEVVGAIYVVASMEDVFNQMKTINTILASGTGLALVLTALLGIFLARTITHPLSDMRKQAMELAKGNFSRKVKKYGHDEIGQLATTFNHLTRELEDAQAMTEGERRKLASVIAYMTDGVIATNRNGAIILLNSPALELLNVSRETALEMPITSLLGLQENYTFEDLVEQQDSMLLEIERDDELTVLRVNFSVIQREHGKIDGLIAVIYDVTEQEKMDQERREFVANVSHELRTPLTTMRSYLEALAEGAWENKDIAPRFLMVTQNETERMIRLVNDLLQLSKFDSKDYQFNREWIQIVRFMSLIIDRFEMTKEQHVEFIRNLPDRDLYVEIDQDKITQVLDNIISNALKYSPEGGHVTFSIDVNEEEELLYISVKDEGIGIPKKDVEKVFDRFYRVDKARTRKLGGTGLGLAIAKEMVQAHGGDIWADSIEGKGTTITFTLPYKEEQEDDWDEA"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAATAAAGT TGGTTTTTTC CGCAGCATCC
     2041 AGTTCAAAAT CACCTTGATT TATGTCCTGC TGATTATTAT TGCCATGCAA ATTATCGGTG
     2101 TTTATTTTGT TAACCAGGTC GAGAAAAGCC TGATTTCCAG CTATGAGCAA AGCTTGAACC
     2161 AGCGCATCGA TAATCTGAGC TACTACATCG AGCAGGAATA TAAAAGCGAC AACGACAGCA
     2221 CTGTTATCAA AGATGACGTC AGCCGTATCC TGAATGACTT TACCAAATCA GATGAAGTCC
     2281 GTGAGATTTC CTTTGTTGAT AAGAGCTACG AGGTTGTCGG TAGCTCCAAA CCTTACGGCG
     2341 AGGAGGTTGC GGGTAAACAA ACGACGGACT TGATCTTTAA ACGTATCTTC TCTACCAAAC
     2401 AAAGCTATCT GCGCAAATAT TATGATCCGA AAAGCAAGAT CCGTGTTCTG ATCAGCGCAA
     2461 AACCGGTGAT GACCGAGAAC CAGGAAGTTG TTGGCGCAAT CTATGTCGTC GCATCTATGG
     2521 AAGATGTTTT CAACCAAATG AAAACGATTA ACACGATCCT GGCGTCGGGC ACTGGTCTGG
     2581 CGTTGGTGCT GACGGCGCTC CTGGGTATTT TCTTGGCCCG CACCATCACC CACCCTCTGT
     2641 CAGACATGCG TAAACAGGCC ATGGAACTGG CAAAAGGCAA CTTTAGCCGT AAAGTTAAAA
     2701 AGTACGGCCA CGACGAGATT GGTCAGCTGG CAACCACCTT TAACCACCTG ACGCGTGAGC
     2761 TGGAGGATGC GCAGGCAATG ACCGAAGGCG AGCGTCGTAA GCTGGCCAGC GTGATTGCCT
     2821 ATATGACCGA CGGTGTGATC GCGACGAACC GTAACGGTGC GATCATTCTG CTGAACTCCC
     2881 CAGCATTAGA GCTGCTGAAC GTCAGCCGTG AAACGGCTTT GGAAATGCCT ATCACGAGCC
     2941 TGCTGGGCCT GCAGGAAAAT TACACCTTCG AGGACTTGGT CGAACAGCAA GATAGCATGT
     3001 TACTGGAAAT CGAGCGTGAT GACGAACTGA CCGTTTTGCG CGTTAACTTT TCAGTGATTC
     3061 AGCGCGAGCA CGGTAAAATT GATGGTCTGA TCGCGGTTAT TTATGACGTC ACTGAGCAAG
     3121 AGAAGATGGA TCAGGAGCGT CGCGAATTCG TTGCGAATGT GTCACACGAA CTGCGTACCC
     3181 CGCTGACCAC CATGCGCTCC TACCTGGAGG CGCTGGCTGA GGGTGCGTGG GAGAATAAGG
     3241 ATATCGCGCC GCGTTTTCTG ATGGTTACGC AGAACGAAAC CGAGCGTATG ATCCGCCTGG
     3301 TTAACGATTT GCTGCAGCTG TCTAAATTTG ACAGCAAGGA CTACCAGTTC AACCGTGAGT
     3361 GGATTCAAAT TGTTCGCTTT ATGTCTCTGA TTATCGATCG CTTCGAAATG ACCAAGGAGC
     3421 AGCATGTTGA ATTCATCCGC AATCTGCCGG ATCGCGACCT GTACGTCGAA ATTGATCAAG
     3481 ACAAAATTAC CCAAGTCCTG GATAATATTA TCAGCAACGC CCTGAAGTAC TCGCCGGAGG
     3541 GCGGTCACGT GACCTTTAGC ATCGATGTGA ATGAGGAAGA GGAGCTGTTG TACATTAGCG
     3601 TCAAAGATGA GGGCATCGGC ATTCCGAAAA AAGACGTGGA GAAGGTTTTT GACCGTTTTT
     3661 ATCGTGTTGA CAAAGCAAGA ACGCGTAAAC TGGGTGGTAC GGGCCTGGGT CTGGCTATCG
     3721 CCAAGGAAAT GGTTCAGGCG CACGGTGGCG ACATTTGGGC CGACAGCATT GAGGGCAAAG
     3781 GTACGACCAT CACCTTCACC CTGCCATACA AGGAAGAACA GGAGGACGAC TGGGACGAAG
     3841 CCTGAAGAGC TTAGAGACC
//