LOCUS       Exported     3896 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3896
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3802
                     /label="aspS"
                     /note="Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp). Also mischarges tRNA(Asp) with D-aspartate, although it is a poor substrate (PubMed:10918062)."
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGIVQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTIINRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDIETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAERRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQIDEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMIFFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHPFTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEEEQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTEAPSFANPTALAELSIQVVKKAENN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    3837..3896
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCTAA TGCGTACCGA
     2041 ATACTGCGGA CAGCTACGTC TATCACACGT CGGACAGCAG GTCACCCTAT GCGGATGGGT
     2101 CAACCGTCGT CGTGACCTAG GATCACTAAT CTTCATCGAC ATGCGTGACC GTGAAGGAAT
     2161 CGTCCAGGTC TTCTTCGACC CGGACCGTGC AGACGCACTA AAACTAGCAT CAGAACTACG
     2221 TAACGAATTC TGCATCCAGG TCACCGGAAC CGTCCGTGCA CGTGACGAAA AAAACATCAA
     2281 CCGTGACATG GCAACCGGAG AAATCGAAGT CCTAGCATCA TCACTAACCA TCATCAACCG
     2341 TGCAGACGTC CTACCGCTAG ACTCAAACCA CGTCAACACC GAAGAAGCAC GTCTAAAATA
     2401 CCGTTACCTA GACCTACGTC GTCCGGAAAT GGCACAGCGT CTAAAAACCC GTGCAAAAAT
     2461 CACCTCACTA GTCCGTCGTT TCATGGACGA CCACGGATTC CTAGACATCG AAACCCCGAT
     2521 GCTAACCAAA GCAACCCCGG AAGGAGCACG TGACTACCTA GTCCCGTCAC GTGTCCACAA
     2581 AGGAAAATTC TACGCACTAC CGCAGTCACC GCAGCTATTC AAACAGCTAC TAATGATGTC
     2641 AGGATTCGAC CGTTACTACC AGATCGTCAA ATGCTTCCGT GACGAAGACC TACGTGCAGA
     2701 CCGTCAGCCG GAATTCACCC AGATCGACGT CGAAACCTCA TTCATGACCG CACCGCAGGT
     2761 CCGTGAAGTC ATGGAAGCAC TAGTCCGTCA CCTATGGCTA GAAGTCAAAG GAGTCGACCT
     2821 AGGAGACTTC CCGGTCATGA CCTTCGCAGA AGCAGAACGT CGTTACGGAT CAGACAAACC
     2881 GGACCTACGT AACCCGATGG AACTAACCGA CGTCGCAGAC CTACTAAAAT CAGTCGAATT
     2941 CGCAGTCTTC GCAGGACCGG CAAACGACCC GAAAGGACGT GTCGCAGCAC TACGTGTCCC
     3001 GGGAGGAGCA TCACTAACCC GTAAACAGAT CGACGAATAC GGAAACTTCG TCAAAATCTA
     3061 CGGAGCAAAA GGACTAGCAT ACATCAAAGT CAACGAACGT GCAAAAGGAC TAGAAGGAAT
     3121 CAACTCACCG GTCGCAAAAT TCCTAAACGC AGAAATCATC GAAGACATCC TAGACCGTAC
     3181 CGCAGCACAG GACGGAGACA TGATCTTCTT CGGAGCAGAC AACAAAAAAA TCGTCGCAGA
     3241 CGCAATGGGA GCACTACGTC TAAAAGTCGG AAAAGACCTA GGACTAACCG ACGAATCAAA
     3301 ATGGGCACCG CTATGGGTCA TCGACTTCCC GATGTTCGAA GACGACGGAG AAGGAGGACT
     3361 AACCGCAATG CACCACCCGT TCACCTCACC GAAAGACATG ACCGCAGCAG AACTAAAAGC
     3421 AGCACCGGAA AACGCAGTCG CAAACGCATA CGACATGGTC ATCAACGGAT ACGAAGTCGG
     3481 AGGAGGATCA GTCCGTATCC ACAACGGAGA CATGCAGCAG ACCGTCTTCG GAATCCTAGG
     3541 AATCAACGAA GAAGAACAGC GTGAAAAATT CGGATTCCTA CTAGACGCAC TAAAATACGG
     3601 AACCCCGCCG CACGCAGGAC TAGCATTCGG ACTAGACCGT CTAACCATGC TACTAACCGG
     3661 AACCGACAAC ATCCGTGACG TCATCGCATT CCCGAAAACC ACCGCAGCAG CATGCCTAAT
     3721 GACCGAAGCA CCGTCATTCG CAAACCCGAC CGCACTAGCA GAACTATCAA TCCAGGTCGT
     3781 CAAAAAAGCA GAAAACAACT GAAGAGCTTC GAGACCATTC AAGGGTTGGA CGACTCTTAT
     3841 GCTACAATTA GACTGCATAG GGATAACAGG GTAATGACCC ACTGGTTTGA AGGCGA
//