LOCUS       Exported     3884 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3884
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3883
                     /label="yejM"
                     /note=""
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWPTTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLLIDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKLRSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFLEKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFEKQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITALNQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNRWFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAGRGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTPASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDEKPQLSLLLQVLTDEKRFIAN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    3825..3884
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCTAA TGGTCACCCA
     2041 CCGTCAGCGT TACCGTGAAA AAGTCTCACA GATGGTCTCA TGGGGACACT GGTTCGCACT
     2101 ATTCAACATC CTACTATCAC TAGTCATCGG ATCACGTTAC CTATTCATCG CAGACTGGCC
     2161 GACCACCCTA GCAGGACGTA TCTACTCATA CGTCTCAATC ATCGGACACT TCTCATTCCT
     2221 AGTCTTCGCA ACCTACCTAC TAATCCTATT CCCGCTAACC TTCATCGTCG GATCACAGCG
     2281 TCTAATGCGT TTCCTATCAG TCATCCTAGC AACCGCAGGA ATGACCCTAC TACTAATCGA
     2341 CTCAGAAGTC TTCACCCGTT TCCACCTACA CCTAAACCCG ATCGTCTGGC AGCTAGTCAT
     2401 CAACCCGGAC GAAAACGAAA TGGCACGTGA CTGGCAGCTA ATGTTCATCT CAGTCCCGGT
     2461 CATCCTACTA CTAGAACTAG TCTTCGCAAC CTGGTCATGG CAGAAACTAC GTTCACTAAC
     2521 CCGTCGTCGT CGTTTCGCAC GTCCGCTAGC AGCATTCCTA TTCATCGCAT TCATCGCATC
     2581 ACACGTCGTC TACATCTGGG CAGACGCAAA CTTCTACCGT CCGATCACCA TGCAGCGTGC
     2641 AAACCTACCG CTATCATACC CGATGACCGC ACGTCGTTTC CTAGAAAAAC ACGGACTACT
     2701 AGACGCACAG GAATACCAGC GTCGTCTAAT CGAACAGGGA AACCCGGACG CAGTCTCAGT
     2761 CCAGTACCCG CTATCAGAAC TACGTTACCG TGACATGGGA ACCGGACAGA ACGTCCTACT
     2821 AATCACCGTC GACGGACTAA ACTACTCACG TTTCGAAAAA CAGATGCCGG CACTAGCAGG
     2881 ATTCGCAGAA CAGAACATCT CATTCACCCG TCACATGTCA TCAGGAAACA CCACCGACAA
     2941 CGGAATCTTC GGACTATTCT ACGGAATCTC ACCGTCATAC ATGGACGGAA TCCTATCAAC
     3001 CCGTACCCCG GCAGCACTAA TCACCGCACT AAACCAGCAG GGATACCAGC TAGGACTATT
     3061 CTCATCAGAC GGATTCACCT CACCGCTATA CCGTCAGGCA CTACTATCAG ACTTCTCAAT
     3121 GCCGTCAGTC CGTACCCAGT CAGACGAACA GACCGCAACC CAGTGGATCA ACTGGCTAGG
     3181 ACGTTACGCA CAGGAAGACA ACCGTTGGTT CTCATGGGTC TCATTCAACG GAACCAACAT
     3241 CGACGACTCA AACCAGCAGG CATTCGCACG TAAATACTCA CGTGCAGCAG GAAACGTCGA
     3301 CGACCAGATC AACCGTGTCC TAAACGCACT ACGTGACTCA GGAAAACTAG ACAACACCGT
     3361 CGTCATCATC ACCGCAGGAC GTGGAATCCC GCTATCAGAA GAAGAAGAAA CCTTCGACTG
     3421 GTCACACGGA CACCTACAGG TCCCGCTAGT CATCCACTGG CCGGGAACCC CGGCACAGCG
     3481 TATCAACGCA CTAACCGACC ACACCGACCT AATGACCACC CTAATGCAGC GTCTACTACA
     3541 CGTCTCAACC CCGGCATCAG AATACTCACA GGGACAGGAC CTATTCAACC CGCAGCGTCG
     3601 TCACTACTGG GTCACCGCAG CAGACAACGA CACCCTAGCA ATCACCACCC CGAAAAAAAC
     3661 CCTAGTCCTA AACAACAACG GAAAATACCG TACCTACAAC CTACGTGGAG AACGTGTCAA
     3721 AGACGAAAAA CCGCAGCTAT CACTACTACT ACAGGTCCTA ACCGACGAAA AACGTTTCAT
     3781 CGCAAACTGA AGAGCTTCGA GACCAGATTG TTACTCCGAC GGACACACTC GCGTCTATCG
     3841 TCCCGTAGGG ATAACAGGGT AATGAGCCGG ATTTCTCTCC CTGA
//