LOCUS       Exported     4751 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4751
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..4750
                     /label="gyrA"
                     /note="Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner."
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMPTKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFPTAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEKIAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINMVALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDPIIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYLTEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELELVREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKGKSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLPLEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDELIGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRGDGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMITDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAAEGDDEIAPEVDVDDEPEEE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    4692..4751
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCTAA TGTCAGACCT
     2041 AGCACGTGAA ATCACCCCGG TCAACATCGA AGAAGAACTA AAATCATCAT ACCTAGACTA
     2101 CGCAATGTCA GTCATCGTCG GACGTGCACT ACCGGACGTC CGTGACGGAC TAAAACCGGT
     2161 CCACCGTCGT GTCCTATACG CAATGAACGT CCTAGGAAAC GACTGGAACA AAGCATACAA
     2221 AAAATCAGCA CGTGTCGTCG GAGACGTCAT CGGAAAATAC CACCCGCACG GAGACTCAGC
     2281 AGTCTACGAC ACCATCGTCC GTATGGCACA GCCGTTCTCA CTACGTTACA TGCTAGTCGA
     2341 CGGACAGGGA AACTTCGGAT CAATCGACGG AGACTCAGCA GCAGCAATGC GTTACACCGA
     2401 AATCCGTCTA GCAAAAATCG CACACGAACT AATGGCAGAC CTAGAAAAAG AAACCGTCGA
     2461 CTTCGTCGAC AACTACGACG GAACCGAAAA AATCCCGGAC GTCATGCCGA CCAAAATCCC
     2521 GAACCTACTA GTCAACGGAT CATCAGGAAT CGCAGTCGGA ATGGCAACCA ACATCCCGCC
     2581 GCACAACCTA ACCGAAGTCA TCAACGGATG CCTAGCATAC ATCGACGACG AAGACATCTC
     2641 AATCGAAGGA CTAATGGAAC ACATCCCGGG ACCGGACTTC CCGACCGCAG CAATCATCAA
     2701 CGGACGTCGT GGAATCGAAG AAGCATACCG TACCGGACGT GGAAAAGTCT ACATCCGTGC
     2761 ACGTGCAGAA GTCGAAGTCG ACGCAAAAAC CGGACGTGAA ACCATCATCG TCCACGAAAT
     2821 CCCGTACCAG GTCAACAAAG CACGTCTAAT CGAAAAAATC GCAGAACTAG TCAAAGAAAA
     2881 ACGTGTCGAA GGAATCTCAG CACTACGTGA CGAATCAGAC AAAGACGGAA TGCGTATCGT
     2941 CATCGAAGTC AAACGTGACG CAGTCGGAGA AGTCGTCCTA AACAACCTAT ACTCACAGAC
     3001 CCAGCTACAG GTCTCATTCG GAATCAACAT GGTCGCACTA CACCACGGAC AGCCGAAAAT
     3061 CATGAACCTA AAAGACATCA TCGCAGCATT CGTCCGTCAC CGTCGTGAAG TCGTCACCCG
     3121 TCGTACCATC TTCGAACTAC GTAAAGCACG TGACCGTGCA CACATCCTAG AAGCACTAGC
     3181 AGTCGCACTA GCAAACATCG ACCCGATCAT CGAACTAATC CGTCACGCAC CGACCCCGGC
     3241 AGAAGCAAAA ACCGCACTAG TCGCAAACCC GTGGCAGCTA GGAAACGTCG CAGCAATGCT
     3301 AGAACGTGCA GGAGACGACG CAGCACGTCC GGAATGGCTA GAACCGGAAT TCGGAGTCCG
     3361 TGACGGACTA TACTACCTAA CCGAACAGCA GGCACAGGCA ATCCTAGACC TACGTCTACA
     3421 GAAACTAACC GGACTAGAAC ACGAAAAACT ACTAGACGAA TACAAAGAAC TACTAGACCA
     3481 GATCGCAGAA CTACTACGTA TCCTAGGATC AGCAGACCGT CTAATGGAAG TCATCCGTGA
     3541 AGAACTAGAA CTAGTCCGTG AACAGTTCGG AGACAAACGT CGTACCGAAA TCACCGCAAA
     3601 CTCAGCAGAC ATCAACCTAG AAGACCTAAT CACCCAGGAA GACGTCGTCG TCACCCTATC
     3661 ACACCAGGGA TACGTCAAAT ACCAGCCGCT ATCAGAATAC GAAGCACAGC GTCGTGGAGG
     3721 AAAAGGAAAA TCAGCAGCAC GTATCAAAGA AGAAGACTTC ATCGACCGTC TACTAGTCGC
     3781 AAACACCCAC GACCACATCC TATGCTTCTC ATCACGTGGA CGTGTCTACT CAATGAAAGT
     3841 CTACCAGCTA CCGGAAGCAA CCCGTGGAGC ACGTGGACGT CCGATCGTCA ACCTACTACC
     3901 GCTAGAACAG GACGAACGTA TCACCGCAAT CCTACCGGTC ACCGAATTCG AAGAAGGAGT
     3961 CAAAGTCTTC ATGGCAACCG CAAACGGAAC CGTCAAAAAA ACCGTCCTAA CCGAATTCAA
     4021 CCGTCTACGT ACCGCAGGAA AAGTCGCAAT CAAACTAGTC GACGGAGACG AACTAATCGG
     4081 AGTCGACCTA ACCTCAGGAG AAGACGAAGT CATGCTATTC TCAGCAGAAG GAAAAGTCGT
     4141 CCGTTTCAAA GAATCATCAG TCCGTGCAAT GGGATGCAAC ACCACCGGAG TCCGTGGAAT
     4201 CCGTCTAGGA GAAGGAGACA AAGTCGTCTC ACTAATCGTC CCGCGTGGAG ACGGAGCAAT
     4261 CCTAACCGCA ACCCAGAACG GATACGGAAA ACGTACCGCA GTCGCAGAAT ACCCGACCAA
     4321 ATCACGTGCA ACCAAAGGAG TCATCTCAAT CAAAGTCACC GAACGTAACG GACTAGTCGT
     4381 CGGAGCAGTC CAGGTCGACG ACTGCGACCA GATCATGATG ATCACCGACG CAGGAACCCT
     4441 AGTCCGTACC CGTGTCTCAG AAATCTCAAT CGTCGGACGT AACACCCAGG GAGTCATCCT
     4501 AATCCGTACC GCAGAAGACG AAAACGTCGT CGGACTACAG CGTGTCGCAG AACCGGTCGA
     4561 CGAAGAAGAC CTAGACACCA TCGACGGATC AGCAGCAGAA GGAGACGACG AAATCGCACC
     4621 GGAAGTCGAC GTCGACGACG AACCGGAAGA AGAATGAAGA GCTTCGAGAC CCCGGTTGTA
     4681 CCTATCGAGT GGCAAATACG GGAGTTACCG TTTAGGGATA ACAGGGTAAT TATCGTACAA
     4741 TAATGTGCGA C
//