LOCUS       Exported     3344 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3344
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1381..1428
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3276..3292
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3293..3324
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1249
                     /label="fabB"
                     /note="Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids."
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGMRSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLIAGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSISSACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKASRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEGAVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHSLGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGFGGTNATLVMRKLKD"
     primer_bind     complement(1433..1449)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1516..2104)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2295..3155)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3156..3260)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1284..1343
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCTAAT GAAACGTGCA GTCATCACCG GACTAGGAAT
       61 CGTCTCATCA ATCGGAAACA ACCAGCAGGA AGTCCTAGCA TCACTACGTG AAGGACGTTC
      121 AGGAATCACC TTCTCACAGG AACTAAAAGA CTCAGGAATG CGTTCACACG TCTGGGGAAA
      181 CGTCAAACTA GACACCACCG GACTAATCGA CCGTAAAGTC GTCCGTTTCA TGTCAGACGC
      241 ATCAATCTAC GCATTCCTAT CAATGGAACA GGCAATCGCA GACGCAGGAC TATCACCGGA
      301 AGCATACCAG AACAACCCGC GTGTCGGACT AATCGCAGGA TCAGGAGGAG GATCACCGCG
      361 TTTCCAGGTC TTCGGAGCAG ACGCAATGCG TGGACCGCGT GGACTAAAAG CAGTCGGACC
      421 GTACGTCGTC ACCAAAGCAA TGGCATCAGG AGTCTCAGCA TGCCTAGCAA CCCCGTTCAA
      481 AATCCACGGA GTCAACTACT CAATCTCATC AGCATGCGCA ACCTCAGCAC ACTGCATCGG
      541 AAACGCAGTC GAACAGATCC AGCTAGGAAA ACAGGACATC GTCTTCGCAG GAGGAGGAGA
      601 AGAACTATGC TGGGAAATGG CATGCGAATT CGACGCAATG GGAGCACTAT CAACCAAATA
      661 CAACGACACC CCGGAAAAAG CATCACGTAC CTACGACGCA CACCGTGACG GATTCGTCAT
      721 CGCAGGAGGA GGAGGAATGG TCGTCGTCGA AGAACTAGAA CACGCACTAG CACGTGGAGC
      781 ACACATCTAC GCAGAAATCG TCGGATACGG AGCAACCTCA GACGGAGCAG ACATGGTCGC
      841 ACCGTCAGGA GAAGGAGCAG TCCGTTGCAT GAAAATGGCA ATGCACGGAG TCGACACCCC
      901 GATCGACTAC CTAAACTCAC ACGGAACCTC AACCCCGGTC GGAGACGTCA AAGAACTAGC
      961 AGCAATCCGT GAAGTCTTCG GAGACAAATC ACCGGCAATC TCAGCAACCA AAGCAATGAC
     1021 CGGACACTCA CTAGGAGCAG CAGGAGTCCA GGAAGCAATC TACTCACTAC TAATGCTAGA
     1081 ACACGGATTC ATCGCACCGT CAATCAACAT CGAAGAACTA GACGAACAGG CAGCAGGACT
     1141 AAACATCGTC ACCGAAACCA CCGACCGTGA ACTAACCACC GTCATGTCAA ACTCATTCGG
     1201 ATTCGGAGGA ACCAACGCAA CCCTAGTCAT GCGTAAACTA AAAGACTGAA GAGCTTCGAG
     1261 ACCACAGAAC GAACAGGCAC TACAGGACTT AGGTTCGTTA CAATTAGGGA TAACAGGGTA
     1321 ATTTATTAGT TGCGGCAACA GTCCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1381 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1441 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1501 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1561 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1621 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1681 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1741 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1801 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1861 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1921 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1981 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     2041 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     2101 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     2161 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     2221 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     2281 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     2341 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     2401 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     2461 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     2521 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     2581 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     2641 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     2701 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     2761 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     2821 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     2881 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     2941 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     3001 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     3061 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     3121 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     3181 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     3241 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     3301 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAG
//