LOCUS       Exported     3539 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3539
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1576..1623
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3471..3487
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3488..3519
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3538
                     /label="gltX"
                     /note="Phosphorylation of GltX by HipA prevents it from being charged, leading to an increase in uncharged tRNA(Glu). This induces amino acid starvation and the stringent response via RelA/SpoT and increased (p)ppGpp levels, which inhibits replication, transcription, translation and cell wall synthesis, reducing growth and leading to multidrug resistance and persistence (PubMed:24095282, PubMed:24343429). Overexpression of GltX prevents HipA-induced growth arrest, persister formation and increases in (p)ppGpp levels (PubMed:24343429, PubMed:28430938)."
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPIEFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVRDKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALDFIAERENQQ"
     primer_bind     complement(1628..1644)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1711..2299)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2490..3350)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3351..3455)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1479..1538
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCTAAT GAAAATCAAA ACCCGTTTCG CACCGTCACC
       61 GACCGGATAC CTACACGTCG GAGGAGCACG TACCGCACTA TACTCATGGC TATTCGCACG
      121 TAACCACGGA GGAGAATTCG TCCTACGTAT CGAAGACACC GACCTAGAAC GTTCAACCCC
      181 GGAAGCAATC GAAGCAATCA TGGACGGAAT GAACTGGCTA TCACTAGAAT GGGACGAAGG
      241 ACCGTACTAC CAGACCAAAC GTTTCGACCG TTACAACGCA GTCATCGACC AGATGCTAGA
      301 AGAAGGAACC GCATACAAAT GCTACTGCTC AAAAGAACGT CTAGAAGCAC TACGTGAAGA
      361 ACAGATGGCA AAAGGAGAAA AACCGCGTTA CGACGGACGT TGCCGTCACT CACACGAACA
      421 CCACGCAGAC GACGAACCGT GCGTCGTCCG TTTCGCAAAC CCGCAGGAAG GATCAGTCGT
      481 CTTCGACGAC CAGATCCGTG GACCGATCGA ATTCTCAAAC CAGGAACTAG ACGACCTAAT
      541 CATCCGTCGT ACCGACGGAT CACCGACCTA CAACTTCTGC GTCGTCGTCG ACGACTGGGA
      601 CATGGAAATC ACCCACGTCA TCCGTGGAGA AGACCACATC AACAACACCC CGCGTCAGAT
      661 CAACATCCTA AAAGCACTAA AAGCACCGGT CCCGGTCTAC GCACACGTCT CAATGATCAA
      721 CGGAGACGAC GGAAAAAAAC TATCAAAACG TCACGGAGCA GTCTCAGTCA TGCAGTACCG
      781 TGACGACGGA TACCTACCGG AAGCACTACT AAACTACCTA GTCCGTCTAG GATGGTCACA
      841 CGGAGACCAG GAAATCTTCA CCCGTGAAGA AATGATCAAA TACTTCACCC TAAACGCAGT
      901 CTCAAAATCA GCATCAGCAT TCAACACCGA CAAACTACTA TGGCTAAACC ACCACTACAT
      961 CAACGCACTA CCGCCGGAAT ACGTCGCAAC CCACCTACAG TGGCACATCG AACAGGAAAA
     1021 CATCGACACC CGTAACGGAC CGCAGCTAGC AGACCTAGTC AAACTACTAG GAGAACGTTG
     1081 CAAAACCCTA AAAGAAATGG CACAGTCATG CCGTTACTTC TACGAAGACT TCGCAGAATT
     1141 CGACGCAGAC GCAGCAAAAA AACACCTACG TCCGGTCGCA CGTCAGCCGC TAGAAGTCGT
     1201 CCGTGACAAA CTAGCAGCAA TCACCGACTG GACCGCAGAA AACGTCCACC ACGCAATCCA
     1261 GGCAACCGCA GACGAACTAG AAGTCGGAAT GGGAAAAGTC GGAATGCCGC TACGTGTCGC
     1321 AGTCACCGGA GCAGGACAGT CACCGGCACT AGACGTCACC GTCCACGCAA TCGGAAAAAC
     1381 CCGTTCAATC GAACGTATCA ACAAAGCACT AGACTTCATC GCAGAACGTG AAAACCAGCA
     1441 GTGAAGAGCT TCGAGACCAC ATTAAATTTC GCCGTGGCTG CGGCTCAGGA CACTTCCAGT
     1501 AGGGATAACA GGGTAATAGA CTTACACAGT CATAAATGCG CTGAGGTGTC AATCGTCGGA
     1561 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1621 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1681 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1741 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1801 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1861 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1921 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1981 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     2041 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     2101 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     2161 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     2221 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     2281 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     2341 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     2401 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2461 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2521 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2581 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2641 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2701 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2761 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2821 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2881 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2941 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     3001 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     3061 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     3121 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     3181 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     3241 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     3301 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     3361 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3421 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3481 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//