LOCUS       Exported     4139 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4139
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..4138
                     /label="ligA"
                     /note="DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA."
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    4080..4139
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCTAA TGGAATCAAT
     2041 CGAACAGCAG CTAACCGAAC TACGTACCAC CCTACGTCAC CACGAATACC TATACCACGT
     2101 CATGGACGCA CCGGAAATCC CGGACGCAGA ATACGACCGT CTAATGCGTG AACTACGTGA
     2161 ACTAGAAACC AAACACCCGG AACTAATCAC CCCGGACTCA CCGACCCAGC GTGTCGGAGC
     2221 AGCACCGCTA GCAGCATTCT CACAGATCCG TCACGAAGTC CCGATGCTAT CACTAGACAA
     2281 CGTCTTCGAC GAAGAATCAT TCCTAGCATT CAACAAACGT GTCCAGGACC GTCTAAAAAA
     2341 CAACGAAAAA GTCACCTGGT GCTGCGAACT AAAACTAGAC GGACTAGCAG TCTCAATCCT
     2401 ATACGAAAAC GGAGTCCTAG TCTCAGCAGC AACCCGTGGA GACGGAACCA CCGGAGAAGA
     2461 CATCACCTCA AACGTCCGTA CCATCCGTGC AATCCCGCTA AAACTACACG GAGAAAACAT
     2521 CCCGGCACGT CTAGAAGTCC GTGGAGAAGT CTTCCTACCG CAGGCAGGAT TCGAAAAAAT
     2581 CAACGAAGAC GCACGTCGTA CCGGAGGAAA AGTCTTCGCA AACCCGCGTA ACGCAGCAGC
     2641 AGGATCACTA CGTCAGCTAG ACCCGCGTAT CACCGCAAAA CGTCCGCTAA CCTTCTTCTG
     2701 CTACGGAGTC GGAGTCCTAG AAGGAGGAGA ACTACCGGAC ACCCACCTAG GACGTCTACT
     2761 ACAGTTCAAA AAATGGGGAC TACCGGTCTC AGACCGTGTC ACCCTATGCG AATCAGCAGA
     2821 AGAAGTCCTA GCATTCTACC ACAAAGTCGA AGAAGACCGT CCGACCCTAG GATTCGACAT
     2881 CGACGGAGTC GTCATCAAAG TCAACTCACT AGCACAGCAG GAACAGCTAG GATTCGTCGC
     2941 ACGTGCACCG CGTTGGGCAG TCGCATTCAA ATTCCCGGCA CAGGAACAGA TGACCTTCGT
     3001 CCGTGACGTC GAATTCCAGG TCGGACGTAC CGGAGCAATC ACCCCGGTCG CACGTCTAGA
     3061 ACCGGTCCAC GTCGCAGGAG TCCTAGTCTC AAACGCAACC CTACACAACG CAGACGAAAT
     3121 CGAACGTCTA GGACTACGTA TCGGAGACAA AGTCGTCATC CGTCGTGCAG GAGACGTCAT
     3181 CCCGCAGGTC GTCAACGTCG TCCTATCAGA ACGTCCGGAA GACACCCGTG AAGTCGTCTT
     3241 CCCGACCCAC TGCCCGGTCT GCGGATCAGA CGTCGAACGT GTCGAAGGAG AAGCAGTCGC
     3301 ACGTTGCACC GGAGGACTAA TCTGCGGAGC ACAGCGTAAA GAATCACTAA AACACTTCGT
     3361 CTCACGTCGT GCAATGGACG TCGACGGAAT GGGAGACAAA ATCATCGACC AGCTAGTCGA
     3421 AAAAGAATAC GTCCACACCC CGGCAGACCT ATTCAAACTA ACCGCAGGAA AACTAACCGG
     3481 ACTAGAACGT ATGGGACCGA AATCAGCACA GAACGTCGTC AACGCACTAG AAAAAGCAAA
     3541 AGAAACCACC TTCGCACGTT TCCTATACGC ACTAGGAATC CGTGAAGTCG GAGAAGCAAC
     3601 CGCAGCAGGA CTAGCAGCAT ACTTCGGAAC CCTAGAAGCA CTAGAAGCAG CATCAATCGA
     3661 AGAACTACAG AAAGTCCCGG ACGTCGGAAT CGTCGTCGCA TCACACGTCC ACAACTTCTT
     3721 CGCAGAAGAA TCAAACCGTA ACGTCATCTC AGAACTACTA GCAGAAGGAG TCCACTGGCC
     3781 GGCACCGATC GTCATCAACG CAGAAGAAAT CGACTCACCG TTCGCAGGAA AAACCGTCGT
     3841 CCTAACCGGA TCACTATCAC AGATGTCACG TGACGACGCA AAAGCACGTC TAGTCGAACT
     3901 AGGAGCAAAA GTCGCAGGAT CAGTCTCAAA AAAAACCGAC CTAGTCATCG CAGGAGAAGC
     3961 AGCAGGATCA AAACTAGCAA AAGCACAGGA ACTAGGAATC GAAGTCATCG ACGAAGCAGA
     4021 AATGCTACGT CTACTAGGAT CATGAAGAGC TTCGAGACCA TCCTAGAAAA GGCGAAGGCC
     4081 TCATGTGTAA ATCCGATACC TAGGGATAAC AGGGTAATAT CAGAAGTTAC CTGTGATTC
//