LOCUS       Exported     3479 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3479
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1516..1563
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3411..3427
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3428..3459
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1384
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKIASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAASNTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYLHQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLFRQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNLPAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSRLQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHDPQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRIL"
                     /label="pssA"
                     /note=""
     primer_bind     complement(1568..1584)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1651..2239)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2430..3290)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3291..3395)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1419..1478
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCTAAT GCTATCAAAA TTCAAACGTA ACAAACACCA
       61 GCAGCACCTA GCACAGCTAC CGAAAATCTC ACAGTCAGTC GACGACGTCG ACTTCTTCTA
      121 CGCACCGGCA GACTTCCGTG AAACCCTACT AGAAAAAATC GCATCAGCAA AACAGCGTAT
      181 CTGCATCGTC GCACTATACC TAGAACAGGA CGACGGAGGA AAAGGAATCC TAAACGCACT
      241 ATACGAAGCA AAACGTCAGC GTCCGGAACT AGACGTCCGT GTCCTAGTCG ACTGGCACCG
      301 TGCACAGCGT GGACGTATCG GAGCAGCAGC ATCAAACACC AACGCAGACT GGTACTGCCG
      361 TATGGCACAG GAAAACCCGG GAGTCGACGT CCCGGTCTAC GGAGTCCCGA TCAACACCCG
      421 TGAAGCACTA GGAGTCCTAC ACTTCAAAGG ATTCATCATC GACGACTCAG TCCTATACTC
      481 AGGAGCATCA CTAAACGACG TCTACCTACA CCAGCACGAC AAATACCGTT ACGACCGTTA
      541 CCACCTAATC CGTAACCGTA AAATGTCAGA CATCATGTTC GAATGGGTCA CCCAGAACAT
      601 CATGAACGGA CGTGGAGTCA ACCGTCTAGA CGACGTCAAC CGTCCGAAAT CACCGGAAAT
      661 CAAAAACGAC ATCCGTCTAT TCCGTCAGGA ACTACGTGAC GCAGCATACC ACTTCCAGGG
      721 AGACGCAGAC AACGACCAGC TATCAGTCAC CCCGCTAGTC GGACTAGGAA AATCATCACT
      781 ACTAAACAAA ACCATCTTCC ACCTAATGCC GTGCGCAGAA CAGAAACTAA CCATCTGCAC
      841 CCCGTACTTC AACCTACCGG CAATCCTAGT CCGTAACATC ATCCAGCTAC TACGTGAAGG
      901 AAAAAAAGTC GAAATCATCG TCGGAGACAA AACCGCAAAC GACTTCTACA TCCCGGAAGA
      961 CGAACCGTTC AAAATCATCG GAGCACTACC GTACCTATAC GAAATCAACC TACGTCGTTT
     1021 CCTATCACGT CTACAGTACT ACGTCAACAC CGACCAGCTA GTCGTCCGTC TATGGAAAGA
     1081 CGACGACAAC ACCTACCACC TAAAAGGAAT GTGGGTCGAC GACAAATGGA TGCTAATCAC
     1141 CGGAAACAAC CTAAACCCGC GTGCATGGCG TCTAGACCTA GAAAACGCAA TCCTAATCCA
     1201 CGACCCGCAG CTAGAACTAG CACCGCAGCG TGAAAAAGAA CTAGAACTAA TCCGTGAACA
     1261 CACCACCATC GTCAAACACT ACCGTGACCT ACAGTCAATC GCAGACTACC CGGTCAAAGT
     1321 CCGTAAACTA ATCCGTCGTC TACGTCGTAT CCGTATCGAC CGTCTAATCT CACGTATCCT
     1381 ATGAAGAGCT TCGAGACCAG CTATGATCCC GGTGTAACAT CGGACCGGGG GACAAGCCAT
     1441 AGGGATAACA GGGTAATACT ACGAATTAGC AAGCGAGACG CTGAGGTGTC AATCGTCGGA
     1501 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1561 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1621 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1681 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1741 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1801 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1861 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1921 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1981 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     2041 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     2101 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     2161 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     2221 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     2281 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     2341 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2401 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2461 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2521 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2581 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2641 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2701 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2761 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2821 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2881 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2941 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     3001 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     3061 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     3121 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     3181 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     3241 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     3301 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3361 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3421 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//