LOCUS       Exported     3485 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3485
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1522..1569
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3417..3433
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3434..3465
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1390
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVALPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAVVLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFFPSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETLFVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVGEKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDFLEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPEIIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGFPGR"
                     /label="ffh"
                     /note="Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components."
     primer_bind     complement(1574..1590)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1657..2245)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2436..3296)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3297..3401)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1425..1484
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCTAAT GTTCGACAAC CTAACCGACC GTCTATCACG
       61 TACCCTACGT AACATCTCAG GACGTGGACG TCTAACCGAA GACAACGTCA AAGACACCCT
      121 ACGTGAAGTC CGTATGGCAC TACTAGAAGC AGACGTCGCA CTACCGGTCG TCCGTGAATT
      181 CATCAACCGT GTCAAAGAAA AAGCAGTCGG ACACGAAGTC AACAAATCAC TAACCCCGGG
      241 ACAGGAATTC GTCAAAATCG TCCGTAACGA ACTAGTCGCA GCAATGGGAG AAGAAAACCA
      301 GACCCTAAAC CTAGCAGCAC AGCCGCCGGC AGTCGTCCTA ATGGCAGGAC TACAGGGAGC
      361 AGGAAAAACC ACCTCAGTCG GAAAACTAGG AAAATTCCTA CGTGAAAAAC ACAAAAAAAA
      421 AGTCCTAGTC GTCTCAGCAG ACGTCTACCG TCCGGCAGCA ATCAAACAGC TAGAAACCCT
      481 AGCAGAACAG GTCGGAGTCG ACTTCTTCCC GTCAGACGTC GGACAGAAAC CGGTCGACAT
      541 CGTCAACGCA GCACTAAAAG AAGCAAAACT AAAATTCTAC GACGTCCTAC TAGTCGACAC
      601 CGCAGGACGT CTACACGTCG ACGAAGCAAT GATGGACGAA ATCAAACAGG TCCACGCATC
      661 AATCAACCCG GTCGAAACCC TATTCGTCGT CGACGCAATG ACCGGACAGG ACGCAGCAAA
      721 CACCGCAAAA GCATTCAACG AAGCACTACC GCTAACCGGA GTCGTCCTAA CCAAAGTCGA
      781 CGGAGACGCA CGTGGAGGAG CAGCACTATC AATCCGTCAC ATCACCGGAA AACCGATCAA
      841 ATTCCTAGGA GTCGGAGAAA AAACCGAAGC ACTAGAACCG TTCCACCCGG ACCGTATCGC
      901 ATCACGTATC CTAGGAATGG GAGACGTCCT ATCACTAATC GAAGACATCG AATCAAAAGT
      961 CGACCGTGCA CAGGCAGAAA AACTAGCATC AAAACTAAAA AAAGGAGACG GATTCGACCT
     1021 AAACGACTTC CTAGAACAGC TACGTCAGAT GAAAAACATG GGAGGAATGG CATCACTAAT
     1081 GGGAAAACTA CCGGGAATGG GACAGATCCC GGACAACGTC AAATCACAGA TGGACGACAA
     1141 AGTCCTAGTC CGTATGGAAG CAATCATCAA CTCAATGACC ATGAAAGAAC GTGCAAAACC
     1201 GGAAATCATC AAAGGATCAC GTAAACGTCG TATCGCAGCA GGATGCGGAA TGCAGGTCCA
     1261 GGACGTCAAC CGTCTACTAA AACAGTTCGA CGACATGCAG CGTATGATGA AAAAAATGAA
     1321 AAAAGGAGGA ATGGCAAAAA TGATGCGTTC AATGAAAGGA ATGATGCCGC CGGGATTCCC
     1381 GGGACGTTGA AGAGCTTCGA GACCACAACG AGCAGACCGA ATAGCGGTGT CTGTTCGGAA
     1441 GCACTTAGGG ATAACAGGGT AATTCTGGTG TTCTCTAACC CTGTCGCTGA GGTGTCAATC
     1501 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1561 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1621 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1681 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1741 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1801 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1861 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1921 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1981 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     2041 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     2101 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     2161 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     2221 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     2281 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2341 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2401 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2461 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2521 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2581 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2641 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2701 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2761 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2821 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2881 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2941 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     3001 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     3061 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     3121 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     3181 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     3241 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     3301 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3361 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     3421 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT
     3481 GGGAG
//