LOCUS       Exported     4754 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4754
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2791..2838
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4686..4702
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4703..4734
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..4753
                     /label="alaS"
                     /note="Attaches Ala to transfer-messenger RNA (tmRNA, also known as 10Sa RNA, the product of the ssrA gene). tmRNA plays a major role in rescue of stalled ribosomes via trans-translation."
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNTQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLGSTIIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ"
     primer_bind     complement(2843..2859)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2926..3514)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3705..4565)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4566..4670)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2694..2753
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCTAAT GTCAAAATCA ACCGCAGAAA TCCGTCAGGC
       61 ATTCCTAGAC TTCTTCCACT CAAAAGGACA CCAGGTCGTC GCATCATCAT CACTAGTCCC
      121 GCACAACGAC CCGACCCTAC TATTCACCAA CGCAGGAATG AACCAGTTCA AAGACGTCTT
      181 CCTAGGACTA GACAAACGTA ACTACTCACG TGCAACCACC TCACAGCGTT GCGTCCGTGC
      241 AGGAGGAAAA CACAACGACC TAGAAAACGT CGGATACACC GCACGTCACC ACACCTTCTT
      301 CGAAATGCTA GGAAACTTCT CATTCGGAGA CTACTTCAAA CACGACGCAA TCCAGTTCGC
      361 ATGGGAACTA CTAACCTCAG AAAAATGGTT CGCACTACCG AAAGAACGTC TATGGGTCAC
      421 CGTCTACGAA TCAGACGACG AAGCATACGA AATCTGGGAA AAAGAAGTCG GAATCCCGCG
      481 TGAACGTATC ATCCGTATCG GAGACAACAA AGGAGCACCG TACGCATCAG ACAACTTCTG
      541 GCAGATGGGA GACACCGGAC CGTGCGGACC GTGCACCGAA ATCTTCTACG ACCACGGAGA
      601 CCACATCTGG GGAGGACCGC CGGGATCACC GGAAGAAGAC GGAGACCGTT ACATCGAAAT
      661 CTGGAACATC GTCTTCATGC AGTTCAACCG TCAGGCAGAC GGAACCATGG AACCGCTACC
      721 GAAACCGTCA GTCGACACCG GAATGGGACT AGAACGTATC GCAGCAGTCC TACAGCACGT
      781 CAACTCAAAC TACGACATCG ACCTATTCCG TACCCTAATC CAGGCAGTCG CAAAAGTCAC
      841 CGGAGCAACC GACCTATCAA ACAAATCACT ACGTGTCATC GCAGACCACA TCCGTTCATG
      901 CGCATTCCTA ATCGCAGACG GAGTCATGCC GTCAAACGAA AACCGTGGAT ACGTCCTACG
      961 TCGTATCATC CGTCGTGCAG TCCGTCACGG AAACATGCTA GGAGCAAAAG AAACCTTCTT
     1021 CTACAAACTA GTCGGACCGC TAATCGACGT CATGGGATCA GCAGGAGAAG ACCTAAAACG
     1081 TCAGCAGGCA CAGGTCGAAC AGGTCCTAAA AACCGAAGAA GAACAGTTCG CACGTACCCT
     1141 AGAACGTGGA CTAGCACTAC TAGACGAAGA ACTAGCAAAA CTATCAGGAG ACACCCTAGA
     1201 CGGAGAAACC GCATTCCGTC TATACGACAC CTACGGATTC CCGGTCGACC TAACCGCAGA
     1261 CGTCTGCCGT GAACGTAACA TCAAAGTCGA CGAAGCAGGA TTCGAAGCAG CAATGGAAGA
     1321 ACAGCGTCGT CGTGCACGTG AAGCATCAGG ATTCGGAGCA GACTACAACG CAATGATCCG
     1381 TGTCGACTCA GCATCAGAAT TCAAAGGATA CGACCACCTA GAACTAAACG GAAAAGTCAC
     1441 CGCACTATTC GTCGACGGAA AAGCAGTCGA CGCAATCAAC GCAGGACAGG AAGCAGTCGT
     1501 CGTCCTAGAC CAGACCCCGT TCTACGCAGA ATCAGGAGGA CAGGTCGGAG ACAAAGGAGA
     1561 ACTAAAAGGA GCAAACTTCT CATTCGCAGT CGAAGACACC CAGAAATACG GACAGGCAAT
     1621 CGGACACATC GGAAAACTAG CAGCAGGATC ACTAAAAGTC GGAGACGCAG TCCAGGCAGA
     1681 CGTCGACGAA GCACGTCGTG CACGTATCCG TCTAAACCAC TCAGCAACCC ACCTAATGCA
     1741 CGCAGCACTA CGTCAGGTCC TAGGAACCCA CGTCTCACAG AAAGGATCAC TAGTCAACGA
     1801 CAAAGTCCTA CGTTTCGACT TCTCACACAA CGAAGCAATG AAACCGGAAG AAATCCGTGC
     1861 AGTCGAAGAC CTAGTCAACA CCCAGATCCG TCGTAACCTA CCGATCGAAA CCAACATCAT
     1921 GGACCTAGAA GCAGCAAAAG CAAAAGGAGC AATGGCACTA TTCGGAGAAA AATACGACGA
     1981 ACGTGTCCGT GTCCTATCAA TGGGAGACTT CTCAACCGAA CTATGCGGAG GAACCCACGC
     2041 ATCACGTACC GGAGACATCG GACTATTCCG TATCATCTCA GAATCAGGAA CCGCAGCAGG
     2101 AGTCCGTCGT ATCGAAGCAG TCACCGGAGA AGGAGCAATC GCAACCGTCC ACGCAGACTC
     2161 AGACCGTCTA TCAGAAGTCG CACACCTACT AAAAGGAGAC TCAAACAACC TAGCAGACAA
     2221 AGTCCGTTCA GTCCTAGAAC GTACCCGTCA GCTAGAAAAA GAACTACAGC AGCTAAAAGA
     2281 ACAGGCAGCA GCACAGGAAT CAGCAAACCT ATCATCAAAA GCAATCGACG TCAACGGAGT
     2341 CAAACTACTA GTCTCAGAAC TATCAGGAGT CGAACCGAAA ATGCTACGTA CCATGGTCGA
     2401 CGACCTAAAA AACCAGCTAG GATCAACCAT CATCGTCCTA GCAACCGTCG TCGAAGGAAA
     2461 AGTCTCACTA ATCGCAGGAG TCTCAAAAGA CGTCACCGAC CGTGTCAAAG CAGGAGAACT
     2521 AATCGGAATG GTCGCACAGC AGGTCGGAGG AAAAGGAGGA GGACGTCCGG ACATGGCACA
     2581 GGCAGGAGGA ACCGACGCAG CAGCACTACC GGCAGCACTA GCATCAGTCA AAGGATGGGT
     2641 CTCAGCAAAA CTACAGTGAA GAGCTTCGAG ACCAGATAGA TGCTCCGTCA AGCCACACAT
     2701 GGCGTCAGAC CTTGTAGGGA TAACAGGGTA ATTGACCCCA AGGCACCCTC TGACGCTGAG
     2761 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     2821 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     2881 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     2941 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     3001 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     3061 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     3121 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     3181 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     3241 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     3301 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     3361 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     3421 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     3481 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     3541 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     3601 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     3661 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     3721 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     3781 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     3841 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     3901 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     3961 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     4021 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     4081 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     4141 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     4201 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     4261 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     4321 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     4381 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     4441 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     4501 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     4561 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     4621 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     4681 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC
     4741 AGGTGGAGTG GGAG
//