LOCUS       Exported     3761 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3761
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1798..1845
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3693..3709
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3710..3741
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3760
                     /label="pyrG"
                     /note="Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates."
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRKERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQMAVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPANERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEWEQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVETRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARHVANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVDDSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPWFVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     primer_bind     complement(1850..1866)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1933..2521)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2712..3572)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3573..3677)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1701..1760
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GACCACCAAC TACATCTTCG TCACCGGAGG
       61 AGTCGTCTCA TCACTAGGAA AAGGAATCGC AGCAGCATCA CTAGCAGCAA TCCTAGAAGC
      121 ACGTGGACTA AACGTCACCA TCATGAAACT AGACCCGTAC ATCAACGTCG ACCCGGGAAC
      181 CATGTCACCG ATCCAGCACG GAGAAGTCTT CGTCACCGAA GACGGAGCAG AAACCGACCT
      241 AGACCTAGGA CACTACGAAC GTTTCATCCG TACCAAAATG TCACGTCGTA ACAACTTCAC
      301 CACCGGACGT ATCTACTCAG ACGTCCTACG TAAAGAACGT CGTGGAGACT ACCTAGGAGC
      361 AACCGTCCAG GTCATCCCGC ACATCACCAA CGCAATCAAA GAACGTGTCC TAGAAGGAGG
      421 AGAAGGACAC GACGTCGTCC TAGTCGAAAT CGGAGGAACC GTCGGAGACA TCGAATCACT
      481 ACCGTTCCTA GAAGCAATCC GTCAGATGGC AGTCGAAATC GGACGTGAAC ACACCCTATT
      541 CATGCACCTA ACCCTAGTCC CGTACATGGC AGCATCAGGA GAAGTCAAAA CCAAACCGAC
      601 CCAGCACTCA GTCAAAGAAC TACTATCAAT CGGAATCCAG CCGGACATCC TAATCTGCCG
      661 TTCAGACCGT GCAGTCCCGG CAAACGAACG TGCAAAAATC GCACTATTCT GCAACGTCCC
      721 GGAAAAAGCA GTCATCTCAC TAAAAGACGT CGACTCAATC TACAAAATCC CGGGACTACT
      781 AAAATCACAG GGACTAGACG ACTACATCTG CAAACGTTTC TCACTAAACT GCCCGGAAGC
      841 AAACCTATCA GAATGGGAAC AGGTCATCTT CGAAGAAGCA AACCCGGTCT CAGAAGTCAC
      901 CATCGGAATG GTCGGAAAAT ACATCGAACT ACCGGACGCA TACAAATCAG TCATCGAAGC
      961 ACTAAAACAC GGAGGACTAA AAAACCGTGT CTCAGTCAAC ATCAAACTAA TCGACTCACA
     1021 GGACGTCGAA ACCCGTGGAG TCGAAATCCT AAAAGGACTA GACGCAATCC TAGTCCCGGG
     1081 AGGATTCGGA TACCGTGGAG TCGAAGGAAT GATCACCACC GCACGTTTCG CACGTGAAAA
     1141 CAACATCCCG TACCTAGGAA TCTGCCTAGG AATGCAGGTC GCACTAATCG ACTACGCACG
     1201 TCACGTCGCA AACATGGAAA ACGCAAACTC AACCGAATTC GTCCCGGACT GCAAATACCC
     1261 GGTCGTCGCA CTAATCACCG AATGGCGTGA CGAAAACGGA AACGTCGAAG TCCGTTCAGA
     1321 AAAATCAGAC CTAGGAGGAA CCATGCGTCT AGGAGCACAG CAGTGCCAGC TAGTCGACGA
     1381 CTCACTAGTC CGTCAGCTAT ACAACGCACC GACCATCGTC GAACGTCACC GTCACCGTTA
     1441 CGAAGTCAAC AACATGCTAC TAAAACAGAT CGAAGACGCA GGACTACGTG TCGCAGGACG
     1501 TTCAGGAGAC GACCAGCTAG TCGAAATCAT CGAAGTCCCG AACCACCCGT GGTTCGTCGC
     1561 ATGCCAGTTC CACCCGGAAT TCACCTCAAC CCCGCGTGAC GGACACCCGC TATTCGCAGG
     1621 ATTCGTCAAA GCAGCATCAG AATTCCAGAA ACGTCAGGCA AAATGAAGAG CTTCGAGACC
     1681 ACAAGGAGTC GGCATATCAC TGATCAGTGC GCGGCTCATA ATAGGGATAA CAGGGTAATA
     1741 GGGGTTCACC GTAATACAGA CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1801 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1861 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1921 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1981 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     2041 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     2101 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     2161 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     2221 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     2281 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     2341 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     2401 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2461 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     2521 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     2581 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2641 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2701 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2761 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2821 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2881 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     2941 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     3001 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     3061 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     3121 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     3181 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     3241 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     3301 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     3361 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     3421 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     3481 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     3541 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     3601 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     3661 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     3721 TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA G
//