LOCUS       Exported     4016 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4016
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2053..2100
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3948..3964
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3965..3996
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1921
                     /label="parE"
                     /note="Topoisomerase IV is essential for chromosome segregation; it is the principal protein responsible for decatenating newly replicated chromosomes (PubMed:9334322). It relaxes supercoiled DNA (PubMed:15105144, PubMed:21300644, PubMed:23294697, PubMed:23352267). MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation (PubMed:20921377)."
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRNTGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGDMAEIEV"
     primer_bind     complement(2105..2121)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2188..2776)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2967..3827)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3828..3932)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1956..2015
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GACCCAGACC TACAACGCAG ACGCAATCGA
       61 AGTCCTAACC GGACTAGAAC CGGTCCGTCG TCGTCCGGGA ATGTACACCG ACACCACCCG
      121 TCCGAACCAC CTAGGACAGG AAGTCATCGA CAACTCAGTC GACGAAGCAC TAGCAGGACA
      181 CGCAAAACGT GTCGACGTCA TCCTACACGC AGACCAGTCA CTAGAAGTCA TCGACGACGG
      241 ACGTGGAATG CCGGTCGACA TCCACCCGGA AGAAGGAGTC CCGGCAGTCG AACTAATCCT
      301 ATGCCGTCTA CACGCAGGAG GAAAATTCTC AAACAAAAAC TACCAGTTCT CAGGAGGACT
      361 ACACGGAGTC GGAATCTCAG TCGTCAACGC ACTATCAAAA CGTGTCGAAG TCAACGTCCG
      421 TCGTGACGGA CAGGTCTACA ACATCGCATT CGAAAACGGA GAAAAAGTCC AGGACCTACA
      481 GGTCGTCGGA ACCTGCGGAA AACGTAACAC CGGAACCTCA GTCCACTTCT GGCCGGACGA
      541 AACCTTCTTC GACTCACCGC GTTTCTCAGT CTCACGTCTA ACCCACGTCC TAAAAGCAAA
      601 AGCAGTCCTA TGCCCGGGAG TCGAAATCAC CTTCAAAGAC GAAATCAACA ACACCGAACA
      661 GCGTTGGTGC TACCAGGACG GACTAAACGA CTACCTAGCA GAAGCAGTCA ACGGACTACC
      721 GACCCTACCG GAAAAACCGT TCATCGGAAA CTTCGCAGGA GACACCGAAG CAGTCGACTG
      781 GGCACTACTA TGGCTACCGG AAGGAGGAGA ACTACTAACC GAATCATACG TCAACCTAAT
      841 CCCGACCATG CAGGGAGGAA CCCACGTCAA CGGACTACGT CAGGGACTAC TAGACGCAAT
      901 GCGTGAATTC TGCGAATACC GTAACATCCT ACCGCGTGGA GTCAAACTAT CAGCAGAAGA
      961 CATCTGGGAC CGTTGCGCAT ACGTCCTATC AGTCAAAATG CAGGACCCGC AGTTCGCAGG
     1021 ACAGACCAAA GAACGTCTAT CATCACGTCA GTGCGCAGCA TTCGTCTCAG GAGTCGTCAA
     1081 AGACGCATTC ATCCTATGGC TAAACCAGAA CGTCCAGGCA GCAGAACTAC TAGCAGAAAT
     1141 GGCAATCTCA TCAGCACAGC GTCGTATGCG TGCAGCAAAA AAAGTCGTCC GTAAAAAACT
     1201 AACCTCAGGA CCGGCACTAC CGGGAAAACT AGCAGACTGC ACCGCACAGG ACCTAAACCG
     1261 TACCGAACTA TTCCTAGTCG AAGGAGACTC AGCAGGAGGA TCAGCAAAAC AGGCACGTGA
     1321 CCGTGAATAC CAGGCAATCA TGCCGCTAAA AGGAAAAATC CTAAACACCT GGGAAGTCTC
     1381 ATCAGACGAA GTCCTAGCAT CACAGGAAGT CCACGACATC TCAGTCGCAA TCGGAATCGA
     1441 CCCGGACTCA GACGACCTAT CACAGCTACG TTACGGAAAA ATCTGCATCC TAGCAGACGC
     1501 AGACTCAGAC GGACTACACA TCGCAACCCT ACTATGCGCA CTATTCGTCA AACACTTCCG
     1561 TGCACTAGTC AAACACGGAC ACGTCTACGT CGCACTACCG CCGCTATACC GTATCGACCT
     1621 AGGAAAAGAA GTCTACTACG CACTAACCGA AGAAGAAAAA GAAGGAGTCC TAGAACAGCT
     1681 AAAACGTAAA AAAGGAAAAC CGAACGTCCA GCGTTTCAAA GGACTAGGAG AAATGAACCC
     1741 GATGCAGCTA CGTGAAACCA CCCTAGACCC GAACACCCGT CGTCTAGTCC AGCTAACCAT
     1801 CGACGACGAA GACGACCAGC GTACCGACGC AATGATGGAC ATGCTACTAG CAAAAAAACG
     1861 TTCAGAAGAC CGTCGTAACT GGCTACAGGA AAAAGGAGAC ATGGCAGAAA TCGAAGTCTG
     1921 AAGAGCTTCG AGACCAATGC GTCATTTTAC ACGGCTTGTG AATGACTGGC ACGTTGTAGG
     1981 GATAACAGGG TAATAGTTCT AGCGCTAAGA CCAACCGCTG AGGTGTCAAT CGTCGGAGCC
     2041 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     2101 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     2161 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     2221 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     2281 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     2341 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     2401 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     2461 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     2521 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     2581 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     2641 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     2701 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     2761 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     2821 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     2881 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     2941 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     3001 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     3061 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     3121 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     3181 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     3241 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     3301 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     3361 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     3421 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     3481 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     3541 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     3601 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     3661 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     3721 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     3781 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     3841 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     3901 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     3961 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG
//