LOCUS Exported 4016 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4016 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 2053..2100 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 3948..3964 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3965..3996 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1921 /label="parE" /note="Topoisomerase IV is essential for chromosome segregation; it is the principal protein responsible for decatenating newly replicated chromosomes (PubMed:9334322). It relaxes supercoiled DNA (PubMed:15105144, PubMed:21300644, PubMed:23294697, PubMed:23352267). MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation (PubMed:20921377)." /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRNTGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGDMAEIEV" primer_bind complement(2105..2121) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2188..2776) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2967..3827) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3828..3932) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1956..2015 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GACCCAGACC TACAACGCAG ACGCAATCGA 61 AGTCCTAACC GGACTAGAAC CGGTCCGTCG TCGTCCGGGA ATGTACACCG ACACCACCCG 121 TCCGAACCAC CTAGGACAGG AAGTCATCGA CAACTCAGTC GACGAAGCAC TAGCAGGACA 181 CGCAAAACGT GTCGACGTCA TCCTACACGC AGACCAGTCA CTAGAAGTCA TCGACGACGG 241 ACGTGGAATG CCGGTCGACA TCCACCCGGA AGAAGGAGTC CCGGCAGTCG AACTAATCCT 301 ATGCCGTCTA CACGCAGGAG GAAAATTCTC AAACAAAAAC TACCAGTTCT CAGGAGGACT 361 ACACGGAGTC GGAATCTCAG TCGTCAACGC ACTATCAAAA CGTGTCGAAG TCAACGTCCG 421 TCGTGACGGA CAGGTCTACA ACATCGCATT CGAAAACGGA GAAAAAGTCC AGGACCTACA 481 GGTCGTCGGA ACCTGCGGAA AACGTAACAC CGGAACCTCA GTCCACTTCT GGCCGGACGA 541 AACCTTCTTC GACTCACCGC GTTTCTCAGT CTCACGTCTA ACCCACGTCC TAAAAGCAAA 601 AGCAGTCCTA TGCCCGGGAG TCGAAATCAC CTTCAAAGAC GAAATCAACA ACACCGAACA 661 GCGTTGGTGC TACCAGGACG GACTAAACGA CTACCTAGCA GAAGCAGTCA ACGGACTACC 721 GACCCTACCG GAAAAACCGT TCATCGGAAA CTTCGCAGGA GACACCGAAG CAGTCGACTG 781 GGCACTACTA TGGCTACCGG AAGGAGGAGA ACTACTAACC GAATCATACG TCAACCTAAT 841 CCCGACCATG CAGGGAGGAA CCCACGTCAA CGGACTACGT CAGGGACTAC TAGACGCAAT 901 GCGTGAATTC TGCGAATACC GTAACATCCT ACCGCGTGGA GTCAAACTAT CAGCAGAAGA 961 CATCTGGGAC CGTTGCGCAT ACGTCCTATC AGTCAAAATG CAGGACCCGC AGTTCGCAGG 1021 ACAGACCAAA GAACGTCTAT CATCACGTCA GTGCGCAGCA TTCGTCTCAG GAGTCGTCAA 1081 AGACGCATTC ATCCTATGGC TAAACCAGAA CGTCCAGGCA GCAGAACTAC TAGCAGAAAT 1141 GGCAATCTCA TCAGCACAGC GTCGTATGCG TGCAGCAAAA AAAGTCGTCC GTAAAAAACT 1201 AACCTCAGGA CCGGCACTAC CGGGAAAACT AGCAGACTGC ACCGCACAGG ACCTAAACCG 1261 TACCGAACTA TTCCTAGTCG AAGGAGACTC AGCAGGAGGA TCAGCAAAAC AGGCACGTGA 1321 CCGTGAATAC CAGGCAATCA TGCCGCTAAA AGGAAAAATC CTAAACACCT GGGAAGTCTC 1381 ATCAGACGAA GTCCTAGCAT CACAGGAAGT CCACGACATC TCAGTCGCAA TCGGAATCGA 1441 CCCGGACTCA GACGACCTAT CACAGCTACG TTACGGAAAA ATCTGCATCC TAGCAGACGC 1501 AGACTCAGAC GGACTACACA TCGCAACCCT ACTATGCGCA CTATTCGTCA AACACTTCCG 1561 TGCACTAGTC AAACACGGAC ACGTCTACGT CGCACTACCG CCGCTATACC GTATCGACCT 1621 AGGAAAAGAA GTCTACTACG CACTAACCGA AGAAGAAAAA GAAGGAGTCC TAGAACAGCT 1681 AAAACGTAAA AAAGGAAAAC CGAACGTCCA GCGTTTCAAA GGACTAGGAG AAATGAACCC 1741 GATGCAGCTA CGTGAAACCA CCCTAGACCC GAACACCCGT CGTCTAGTCC AGCTAACCAT 1801 CGACGACGAA GACGACCAGC GTACCGACGC AATGATGGAC ATGCTACTAG CAAAAAAACG 1861 TTCAGAAGAC CGTCGTAACT GGCTACAGGA AAAAGGAGAC ATGGCAGAAA TCGAAGTCTG 1921 AAGAGCTTCG AGACCAATGC GTCATTTTAC ACGGCTTGTG AATGACTGGC ACGTTGTAGG 1981 GATAACAGGG TAATAGTTCT AGCGCTAAGA CCAACCGCTG AGGTGTCAAT CGTCGGAGCC 2041 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG 2101 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT 2161 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 2221 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 2281 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 2341 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 2401 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 2461 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 2521 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 2581 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 2641 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 2701 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 2761 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG 2821 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 2881 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 2941 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 3001 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 3061 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA 3121 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 3181 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 3241 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 3301 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 3361 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 3421 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 3481 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 3541 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 3601 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 3661 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 3721 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 3781 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 3841 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 3901 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC 3961 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG //