LOCUS       Exported     3869 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3869
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1906..1953
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3801..3817
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3818..3849
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3868
                     /label="dnaG"
                     /note="RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication."
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAGSGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGFAPPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQLRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLSTLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTTMRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYRGTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNEERLELWTLNQELAKK"
     primer_bind     complement(1958..1974)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2041..2629)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2820..3680)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3681..3785)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1809..1868
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCTAAT GGCAGGACGT ATCCCGCGTG TCTTCATCAA
       61 CGACCTACTA GCACGTACCG ACATCGTCGA CCTAATCGAC GCACGTGTCA AACTAAAAAA
      121 ACAGGGAAAA AACTTCCACG CATGCTGCCC GTTCCACAAC GAAAAAACCC CGTCATTCAC
      181 CGTCAACGGA GAAAAACAGT TCTACCACTG CTTCGGATGC GGAGCACACG GAAACGCAAT
      241 CGACTTCCTA ATGAACTACG ACAAACTAGA ATTCGTCGAA ACCGTCGAAG AACTAGCAGC
      301 AATGCACAAC CTAGAAGTCC CGTTCGAAGC AGGATCAGGA CCGTCACAGA TCGAACGTCA
      361 CCAGCGTCAG ACCCTATACC AGCTAATGGA CGGACTAAAC ACCTTCTACC AGCAGTCACT
      421 ACAGCAGCCG GTCGCAACCT CAGCACGTCA GTACCTAGAA AAACGTGGAC TATCACACGA
      481 AGTCATCGCA CGTTTCGCAA TCGGATTCGC ACCGCCGGGA TGGGACAACG TCCTAAAACG
      541 TTTCGGAGGA AACCCGGAAA ACCGTCAGTC ACTAATCGAC GCAGGAATGC TAGTCACCAA
      601 CGACCAGGGA CGTTCATACG ACCGTTTCCG TGAACGTGTC ATGTTCCCGA TCCGTGACAA
      661 ACGTGGACGT GTCATCGGAT TCGGAGGACG TGTCCTAGGA AACGACACCC CGAAATACCT
      721 AAACTCACCG GAAACCGACA TCTTCCACAA AGGACGTCAG CTATACGGAC TATACGAAGC
      781 ACAGCAGGAC AACGCAGAAC CGAACCGTCT ACTAGTCGTC GAAGGATACA TGGACGTCGT
      841 CGCACTAGCA CAGTACGGAA TCAACTACGC AGTCGCATCA CTAGGAACCT CAACCACCGC
      901 AGACCACATC CAGCTACTAT TCCGTGCAAC CAACAACGTC ATCTGCTGCT ACGACGGAGA
      961 CCGTGCAGGA CGTGACGCAG CATGGCGTGC ACTAGAAACC GCACTACCGT ACATGACCGA
     1021 CGGACGTCAG CTACGTTTCA TGTTCCTACC GGACGGAGAA GACCCGGACA CCCTAGTCCG
     1081 TAAAGAAGGA AAAGAAGCAT TCGAAGCACG TATGGAACAG GCAATGCCGC TATCAGCATT
     1141 CCTATTCAAC TCACTAATGC CGCAGGTCGA CCTATCAACC CCGGACGGAC GTGCACGTCT
     1201 ATCAACCCTA GCACTACCGC TAATCTCACA GGTCCCGGGA GAAACCCTAC GTATCTACCT
     1261 ACGTCAGGAA CTAGGAAACA AACTAGGAAT CCTAGACGAC TCACAGCTAG AACGTCTAAT
     1321 GCCGAAAGCA GCAGAATCAG GAGTCTCACG TCCGGTCCCG CAGCTAAAAC GTACCACCAT
     1381 GCGTATCCTA ATCGGACTAC TAGTCCAGAA CCCGGAACTA GCAACCCTAG TCCCGCCGCT
     1441 AGAAAACCTA GACGAAAACA AACTACCGGG ACTAGGACTA TTCCGTGAAC TAGTCAACAC
     1501 CTGCCTATCA CAGCCGGGAC TAACCACCGG ACAGCTACTA GAACACTACC GTGGAACCAA
     1561 CAACGCAGCA ACCCTAGAAA AACTATCAAT GTGGGACGAC ATCGCAGACA AAAACATCGC
     1621 AGAACAGACC TTCACCGACT CACTAAACCA CATGTTCGAC TCACTACTAG AACTACGTCA
     1681 GGAAGAACTA ATCGCACGTG AACGTACCCA CGGACTATCA AACGAAGAAC GTCTAGAACT
     1741 ATGGACCCTA AACCAGGAAC TAGCAAAAAA ATGAAGAGCT TCGAGACCAC GAGATGATGC
     1801 ACCGATAGCC GCGGTCGGAT AAGACACGGT AGGGATAACA GGGTAATCGT GACCGCCCGT
     1861 GGTGTCACCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1921 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     1981 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     2041 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     2101 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     2161 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     2221 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     2281 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     2341 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     2401 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     2461 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     2521 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     2581 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     2641 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2701 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     2761 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     2821 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2881 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     2941 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     3001 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     3061 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     3121 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     3181 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     3241 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     3301 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     3361 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     3421 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     3481 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     3541 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     3601 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     3661 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     3721 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     3781 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     3841 CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//