LOCUS       Exported     4058 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4058
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3964
                     /label="ftsH"
                     /note="(Microbial infection) Probably transports the toxic C-terminal region of CdiA from E.coli strain 536, E.cloacae strain ATCC 13047 and of Y.pestis strain A across the inner membrane to the cytoplasm, where CdiA has a toxic effect. Toxin transport is strain-specific, mutations in this gene do not confer resistance to several other tested CdiA toxins."
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNNDQVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASIFISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEAKEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVHMRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKIMMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLPEGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWGFSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTDNMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRPVDEPRTPNPGNTMSEQLGDK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    3999..4058
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCTAA TGGCAAAAAA
     2041 CCTAATCCTA TGGCTAGTCA TCGCAGTCGT CCTAATGTCA GTCTTCCAGT CATTCGGACC
     2101 GTCAGAATCA AACGGACGTA AAGTCGACTA CTCAACCTTC CTACAGGAAG TCAACAACGA
     2161 CCAGGTCCGT GAAGCACGTA TCAACGGACG TGAAATCAAC GTCACCAAAA AAGACTCAAA
     2221 CCGTTACACC ACCTACATCC CGGTCCAGGA CCCGAAACTA CTAGACAACC TACTAACCAA
     2281 AAACGTCAAA GTCGTCGGAG AACCGCCGGA AGAACCGTCA CTACTAGCAT CAATCTTCAT
     2341 CTCATGGTTC CCGATGCTAC TACTAATCGG AGTCTGGATC TTCTTCATGC GTCAGATGCA
     2401 GGGAGGAGGA GGAAAAGGAG CAATGTCATT CGGAAAATCA AAAGCACGTA TGCTAACCGA
     2461 AGACCAGATC AAAACCACCT TCGCAGACGT CGCAGGATGC GACGAAGCAA AAGAAGAAGT
     2521 CGCAGAACTA GTCGAATACC TACGTGAACC GTCACGTTTC CAGAAACTAG GAGGAAAAAT
     2581 CCCGAAAGGA GTCCTAATGG TCGGACCGCC GGGAACCGGA AAAACCCTAC TAGCAAAAGC
     2641 AATCGCAGGA GAAGCAAAAG TCCCGTTCTT CACCATCTCA GGATCAGACT TCGTCGAAAT
     2701 GTTCGTCGGA GTCGGAGCAT CACGTGTCCG TGACATGTTC GAACAGGCAA AAAAAGCAGC
     2761 ACCGTGCATC ATCTTCATCG ACGAAATCGA CGCAGTCGGA CGTCAGCGTG GAGCAGGACT
     2821 AGGAGGAGGA CACGACGAAC GTGAACAGAC CCTAAACCAG ATGCTAGTCG AAATGGACGG
     2881 ATTCGAAGGA AACGAAGGAA TCATCGTCAT CGCAGCAACC AACCGTCCGG ACGTCCTAGA
     2941 CCCGGCACTA CTACGTCCGG GACGTTTCGA CCGTCAGGTC GTCGTCGGAC TACCGGACGT
     3001 CCGTGGACGT GAACAGATCC TAAAAGTCCA CATGCGTCGT GTCCCGCTAG CACCGGACAT
     3061 CGACGCAGCA ATCATCGCAC GTGGAACCCC GGGATTCTCA GGAGCAGACC TAGCAAACCT
     3121 AGTCAACGAA GCAGCACTAT TCGCAGCACG TGGAAACAAA CGTGTCGTCT CAATGGTCGA
     3181 ATTCGAAAAA GCAAAAGACA AAATCATGAT GGGAGCAGAA CGTCGTTCAA TGGTCATGAC
     3241 CGAAGCACAG AAAGAATCAA CCGCATACCA CGAAGCAGGA CACGCAATCA TCGGACGTCT
     3301 AGTCCCGGAA CACGACCCGG TCCACAAAGT CACCATCATC CCGCGTGGAC GTGCACTAGG
     3361 AGTCACCTTC TTCCTACCGG AAGGAGACGC AATCTCAGCA TCACGTCAGA AACTAGAATC
     3421 ACAGATCTCA ACCCTATACG GAGGACGTCT AGCAGAAGAA ATCATCTACG GACCGGAACA
     3481 CGTCTCAACC GGAGCATCAA ACGACATCAA AGTCGCAACC AACCTAGCAC GTAACATGGT
     3541 CACCCAGTGG GGATTCTCAG AAAAACTAGG ACCGCTACTA TACGCAGAAG AAGAAGGAGA
     3601 AGTCTTCCTA GGACGTTCAG TCGCAAAAGC AAAACACATG TCAGACGAAA CCGCACGTAT
     3661 CATCGACCAG GAAGTCAAAG CACTAATCGA ACGTAACTAC AACCGTGCAC GTCAGCTACT
     3721 AACCGACAAC ATGGACATCC TACACGCAAT GAAAGACGCA CTAATGAAAT ACGAAACCAT
     3781 CGACGCACCG CAGATCGACG ACCTAATGGC ACGTCGTGAC GTCCGTCCGC CGGCAGGATG
     3841 GGAAGAACCG GGAGCATCAA ACAACTCAGG AGACAACGGA TCACCGAAAG CACCGCGTCC
     3901 GGTCGACGAA CCGCGTACCC CGAACCCGGG AAACACCATG TCAGAACAGC TAGGAGACAA
     3961 ATGAAGAGCT TCGAGACCAT ACTGTAAGAA CCACGCGGAC ATCCTCCTAA GAACGATAGT
     4021 AGGGATAACA GGGTAATAAC AGCCAATGCC CCGTACTG
//