LOCUS       Exported     3473 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3473
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1510..1557
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3405..3421
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3422..3453
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1378
                     /label="accC"
                     /note="This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA."
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLLADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIFIGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKASGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADGQGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFEFLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHAVECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYGENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     primer_bind     complement(1562..1578)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1645..2233)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2424..3284)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3285..3389)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1413..1472
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACCGGAGC CATACAGTAC GGTCTCTAAT GCTAGACAAA ATCGTCATCG CAAACCGTGG
       61 AGAAATCGCA CTACGTATCC TACGTGCATG CAAAGAACTA GGAATCAAAA CCGTCGCAGT
      121 CCACTCATCA GCAGACCGTG ACCTAAAACA CGTCCTACTA GCAGACGAAA CCGTCTGCAT
      181 CGGACCGGCA CCGTCAGTCA AATCATACCT AAACATCCCG GCAATCATCT CAGCAGCAGA
      241 AATCACCGGA GCAGTCGCAA TCCACCCGGG ATACGGATTC CTATCAGAAA ACGCAAACTT
      301 CGCAGAACAG GTCGAACGTT CAGGATTCAT CTTCATCGGA CCGAAAGCAG AAACCATCCG
      361 TCTAATGGGA GACAAAGTCT CAGCAATCGC AGCAATGAAA AAAGCAGGAG TCCCGTGCGT
      421 CCCGGGATCA GACGGACCGC TAGGAGACGA CATGGACAAA AACCGTGCAA TCGCAAAACG
      481 TATCGGATAC CCGGTCATCA TCAAAGCATC AGGAGGAGGA GGAGGACGTG GAATGCGTGT
      541 CGTCCGTGGA GACGCAGAAC TAGCACAGTC AATCTCAATG ACCCGTGCAG AAGCAAAAGC
      601 AGCATTCTCA AACGACATGG TCTACATGGA AAAATACCTA GAAAACCCGC GTCACGTCGA
      661 AATCCAGGTC CTAGCAGACG GACAGGGAAA CGCAATCTAC CTAGCAGAAC GTGACTGCTC
      721 AATGCAGCGT CGTCACCAGA AAGTCGTCGA AGAAGCACCG GCACCGGGAA TCACCCCGGA
      781 ACTACGTCGT TACATCGGAG AACGTTGCGC AAAAGCATGC GTCGACATCG GATACCGTGG
      841 AGCAGGAACC TTCGAATTCC TATTCGAAAA CGGAGAATTC TACTTCATCG AAATGAACAC
      901 CCGTATCCAG GTCGAACACC CGGTCACCGA AATGATCACC GGAGTCGACC TAATCAAAGA
      961 ACAGCTACGT ATCGCAGCAG GACAGCCGCT ATCAATCAAA CAGGAAGAAG TCCACGTCCG
     1021 TGGACACGCA GTCGAATGCC GTATCAACGC AGAAGACCCG AACACCTTCC TACCGTCACC
     1081 GGGAAAAATC ACCCGTTTCC ACGCACCGGG AGGATTCGGA GTCCGTTGGG AATCACACAT
     1141 CTACGCAGGA TACACCGTCC CGCCGTACTA CGACTCAATG ATCGGAAAAC TAATCTGCTA
     1201 CGGAGAAAAC CGTGACGTCG CAATCGCACG TATGAAAAAC GCACTACAGG AACTAATCAT
     1261 CGACGGAATC AAAACCAACG TCGACCTACA GATCCGTATC ATGAACGACG AAAACTTCCA
     1321 GCACGGAGGA ACCAACATCC ACTACCTAGA AAAAAAACTA GGACTACAGG AAAAATGAAG
     1381 AGCTTCGAGA CCACAGAACG AACAGGCACT ACGCGAACGC GCGACTATCA ATCTAGGGAT
     1441 AACAGGGTAA TGGGTGTCCA TCCAGATATG TCCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1501 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1561 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1621 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1681 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1741 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1801 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1861 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1921 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1981 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2041 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2101 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2161 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2221 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2281 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2341 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2401 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2461 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2521 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2581 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2641 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2701 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2761 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2821 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2881 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2941 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3001 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3061 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3121 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3181 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3241 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3301 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3361 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3421 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAG
//