LOCUS       Exported     4259 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4259
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             100..4258
                     /label="yrfF"
                     /note=""
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRKLTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGISTDDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETRSYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFFCLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQEQINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPLQHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRVGDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATALTEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLKNALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARAAQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNAEGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQRHRTRMMEIQAYYESCLNPQLITPSESLIE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    4200..4259
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGGGGCC GTCAATATCA GGGTCTCTAA TGTCAACCAT
     2041 CGTCATCTTC CTAGCAGCAC TACTAGCATG CTCACTACTA GCAGGATGGC TAATCAAAGT
     2101 CCGTTCACGT CGTCGTCAGC TACCGTGGAC CAACGCATTC GCAGACGCAC AGACCCGTAA
     2161 ACTAACCCCG GAAGAACGTT CAGCAGTCGA AAACTACCTA GAATCACTAA CCCAGGTCCT
     2221 ACAGGTCCCG GGACCGACCG GAGCATCAGC AGCACCGATC TCACTAGCAC TAAACGCAGA
     2281 ATCAAACAAC GTCATGATGC TAACCCACGC AATCACCCGT TACGGAATCT CAACCGACGA
     2341 CCCGAACAAA TGGCGTTACT ACCTAGACTC AGTCGAAGTC CACCTACCGC CGTTCTGGGA
     2401 ACAGTACATC AACGACGAAA ACACCGTCGA ACTAATCCAC ACCGACTCAC TACCGCTAGT
     2461 CATCTCACTA AACGGACACA CCCTACAGGA ATACATGCAG GAAACCCGTT CATACGCACT
     2521 ACAGCCGGTC CCGTCAACCC AGGCATCAAT CCGTGGAGAA GAATCAGAAC AGATCGAACT
     2581 ACTAAACATC CGTAAAGAAA CCCACGAAGA ATACGCACTA TCACGTCCGC GTGGACTACG
     2641 TGAAGCACTA CTAATCGTCG CATCATTCCT AATGTTCTTC TTCTGCCTAA TCACCCCGGA
     2701 CGTCTTCGTC CCGTGGCTAG CAGGAGGAGC ACTACTACTA CTAGGAGCAG GACTATGGGG
     2761 ACTATTCGCA CCGCCGGCAA AATCATCACT ACGTGAAATC CACTGCCTAC GTGGAACCCC
     2821 GCGTCGTTGG GGACTATTCG GAGAAAACGA CCAGGAACAG ATCAACAACA TCTCACTAGG
     2881 AATCATCGAC CTAGTCTACC CGGCACACTG GCAGCCGTAC ATCGCACAGG ACCTAGGACA
     2941 GCAGACCGAC ATCGACATCT ACCTAGACCG TCACGTCGTC CGTCAGGGAC GTTACCTATC
     3001 ACTACACGAC GAAGTCAAAA ACTTCCCGCT ACAGCACTGG CTACGTTCAA CCATCATCGC
     3061 AGCAGGATCA CTACTAGTCC TATTCATGCT ACTATTCTGG ATCCCGCTAG ACATGCCGCT
     3121 AAAATTCACC CTATCATGGA TGAAAGGAGC ACAGACCATC GAAGCAACCT CAGTCAAACA
     3181 GCTAGCAGAC GCAGGAGTCC GTGTCGGAGA CACCCTACGT ATCTCAGGAA CCGGAATGTG
     3241 CAACATCCGT ACCTCAGGAA CCTGGTCAGC AAAAACCAAC TCACCGTTCC TACCGTTCGA
     3301 CTGCTCACAG ATCATCTGGA ACGACGCACG TTCACTACCG CTACCGGAAT CAGAACTAGT
     3361 CAACAAAGCA ACCGCACTAA CCGAAGCAGT CAACCGTCAG CTACACCCGA AACCGGAAGA
     3421 CGAATCACGT GTCTCAGCAT CACTACGTTC AGCAATCCAG AAATCAGGAA TGGTCCTACT
     3481 AGACGACTTC GGAGACATCG TCCTAAAAAC CGCAGACCTA TGCTCAGCAA AAGACGACTG
     3541 CGTCCGTCTA AAAAACGCAC TAGTCAACCT AGGAAACTCA AAAGACTGGG ACGCACTAGT
     3601 CAAACGTGCA AACGCAGGAA AACTAGACGG AGTCAACGTC CTACTACGTC CGGTCTCAGC
     3661 AGAATCACTA GACAACCTAG TCGCAACCTC AACCGCACCG TTCATCACCC ACGAAACCGC
     3721 ACGTGCAGCA CAGTCACTAA ACTCACCGGC ACCGGGAGGA TTCCTAATCG TCTCAGACGA
     3781 AGGATCAGAC TTCGTCGACC AGCCGTGGCC GTCAGCATCA CTATACGACT ACCCGCCGCA
     3841 GGAACAGTGG AACGCATTCC AGAAACTAGC ACAGATGCTA ATGCACACCC CGTTCAACGC
     3901 AGAAGGAATC GTCACCAAAA TCTTCACCGA CGCAAACGGA ACCCAGCACA TCGGACTACA
     3961 CCCGATCCCG GACCGTTCAG GACTATGGCG TTACCTATCA ACCACCCTAC TACTACTAAC
     4021 CATGCTAGGA TCAGCAATCT ACAACGGAGT CCAGGCATGG CGTCGTTACC AGCGTCACCG
     4081 TACCCGTATG ATGGAAATCC AGGCATACTA CGAATCATGC CTAAACCCGC AGCTAATCAC
     4141 CCCGTCAGAA TCACTAATCG AATGAAGAGC TTCGAGACCA AGGATATGTA GACACCGCCC
     4201 GGAAGTAGAT ACACCCGGTT TAGGGATAAC AGGGTAATTA ACGACATTAA AAGTACCTG
//