LOCUS       Exported     3953 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3953
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1990..2037
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3885..3901
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3902..3933
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3952
                     /label="glmS"
                     /note="Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source."
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMTRLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGIIENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTVIMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRRSVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQVDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYRKSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMTNAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     primer_bind     complement(2042..2058)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2125..2713)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2904..3764)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3765..3869)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1893..1952
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCTAAT GTGCGGAATC GTCGGAGCAA TCGCACAGCG
       61 TGACGTCGCA GAAATCCTAC TAGAAGGACT ACGTCGTCTA GAATACCGTG GATACGACTC
      121 AGCAGGACTA GCAGTCGTCG ACGCAGAAGG ACACATGACC CGTCTACGTC GTCTAGGAAA
      181 AGTCCAGATG CTAGCACAGG CAGCAGAAGA ACACCCGCTA CACGGAGGAA CCGGAATCGC
      241 ACACACCCGT TGGGCAACCC ACGGAGAACC GTCAGAAGTC AACGCACACC CGCACGTCTC
      301 AGAACACATC GTCGTCGTCC ACAACGGAAT CATCGAAAAC CACGAACCGC TACGTGAAGA
      361 ACTAAAAGCA CGTGGATACA CCTTCGTCTC AGAAACCGAC ACCGAAGTCA TCGCACACCT
      421 AGTCAACTGG GAACTAAAAC AGGGAGGAAC CCTACGTGAA GCAGTCCTAC GTGCAATCCC
      481 GCAGCTACGT GGAGCATACG GAACCGTCAT CATGGACTCA CGTCACCCGG ACACCCTACT
      541 AGCAGCACGT TCAGGATCAC CGCTAGTCAT CGGACTAGGA ATGGGAGAAA ACTTCATCGC
      601 ATCAGACCAG CTAGCACTAC TACCGGTCAC CCGTCGTTTC ATCTTCCTAG AAGAAGGAGA
      661 CATCGCAGAA ATCACCCGTC GTTCAGTCAA CATCTTCGAC AAAACCGGAG CAGAAGTCAA
      721 ACGTCAGGAC ATCGAATCAA ACCTACAGTA CGACGCAGGA GACAAAGGAA TCTACCGTCA
      781 CTACATGCAG AAAGAAATCT ACGAACAGCC GAACGCAATC AAAAACACCC TAACCGGACG
      841 TATCTCACAC GGACAGGTCG ACCTATCAGA ACTAGGACCG AACGCAGACG AACTACTATC
      901 AAAAGTCGAA CACATCCAGA TCCTAGCATG CGGAACCTCA TACAACTCAG GAATGGTCTC
      961 ACGTTACTGG TTCGAATCAC TAGCAGGAAT CCCGTGCGAC GTCGAAATCG CATCAGAATT
     1021 CCGTTACCGT AAATCAGCAG TCCGTCGTAA CTCACTAATG ATCACCCTAT CACAGTCAGG
     1081 AGAAACCGCA GACACCCTAG CAGGACTACG TCTATCAAAA GAACTAGGAT ACCTAGGATC
     1141 ACTAGCAATC TGCAACGTCC CGGGATCATC ACTAGTCCGT GAATCAGACC TAGCACTAAT
     1201 GACCAACGCA GGAACCGAAA TCGGAGTCGC ATCAACCAAA GCATTCACCA CCCAGCTAAC
     1261 CGTCCTACTA ATGCTAGTCG CAAAACTATC ACGTCTAAAA GGACTAGACG CATCAATCGA
     1321 ACACGACATC GTCCACGGAC TACAGGCACT ACCGTCACGT ATCGAACAGA TGCTATCACA
     1381 GGACAAACGT ATCGAAGCAC TAGCAGAAGA CTTCTCAGAC AAACACCACG CACTATTCCT
     1441 AGGACGTGGA GACCAGTACC CGATCGCACT AGAAGGAGCA CTAAAACTAA AAGAAATCTC
     1501 ATACATCCAC GCAGAAGCAT ACGCAGCAGG AGAACTAAAA CACGGACCGC TAGCACTAAT
     1561 CGACGCAGAC ATGCCGGTCA TCGTCGTCGC ACCGAACAAC GAACTACTAG AAAAACTAAA
     1621 ATCAAACATC GAAGAAGTCC GTGCACGTGG AGGACAGCTA TACGTCTTCG CAGACCAGGA
     1681 CGCAGGATTC GTCTCATCAG ACAACATGCA CATCATCGAA ATGCCGCACG TCGAAGAAGT
     1741 CATCGCACCG ATCTTCTACA CCGTCCCGCT ACAGCTACTA GCATACCACG TCGCACTAAT
     1801 CAAAGGAACC GACGTCGACC AGCCGCGTAA CCTAGCAAAA TCAGTCACCG TCGAATGAAG
     1861 AGCTTCGAGA CCAAGGATAT GTAGACACCG CCCGCGGAAC ATAAAATTCT CCGTAGGGAT
     1921 AACAGGGTAA TTGCTCTTTC AGGGAATTGT CTCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1981 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     2041 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     2101 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     2161 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     2221 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     2281 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     2341 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     2401 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     2461 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2521 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2581 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2641 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2701 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2761 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2821 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2881 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2941 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     3001 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     3061 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     3121 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     3181 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     3241 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     3301 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     3361 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     3421 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3481 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3541 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3601 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3661 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3721 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3781 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3841 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3901 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAG
//