LOCUS       Exported     3476 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3476
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1513..1560
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3408..3424
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3425..3456
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1381
                     /label="wzyE"
                     /note="Probably involved in the polymerization of enterobacterial common antigen (ECA) trisaccharide repeat units. Required for the assembly of the phosphoglyceride-linked form of ECA (ECA(PG)) and the water-soluble cyclic form of ECA (ECA(CYC))."
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFLLTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPRRPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKRFFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIGIIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLALLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAISPTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIFNMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG"
     primer_bind     complement(1565..1581)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1648..2236)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2427..3287)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3288..3392)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1416..1475
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACCGGAGC CATACAGTAC GGTCTCTAAT GTCACTACTA CAGTTCTCAG GACTATTCGT
       61 CGTCTGGCTA CTATGCACCC TATTCATCGC AACCCTAACC TGGTTCGAAT TCCGTCGTGT
      121 CCGTTTCAAC TTCAACGTCT TCTTCTCACT ACTATTCCTA CTAACCTTCT TCTTCGGATT
      181 CCCGCTAACC TCAGTCCTAG TCTTCCGTTT CGACGTCGGA GTCGCACCGC CGGAAATCCT
      241 ACTACAGGCA CTACTATCAG CAGGATGCTT CTACGCAGTC TACTACGTCA CCTACAAAAC
      301 CCGTCTACGT AAACGTGTCG CAGACGTCCC GCGTCGTCCG CTATTCACCA TGAACCGTGT
      361 CGAAACCAAC CTAACCTGGG TCATCCTAAT GGGAATCGCA CTAGTCTCAG TCGGAATCTT
      421 CTTCATGCAC AACGGATTCC TACTATTCCG TCTAAACTCA TACTCACAGA TCTTCTCATC
      481 AGAAGTCTCA GGAGTCGCAC TAAAACGTTT CTTCTACTTC TTCATCCCGG CAATGCTAGT
      541 CGTCTACTTC CTACGTCAGG ACTCAAAAGC ATGGCTATTC TTCCTAGTCT CAACCGTCGC
      601 ATTCGGACTA CTAACCTACA TGATCGTCGG AGGAACCCGT GCAAACATCA TCATCGCATT
      661 CGCAATCTTC CTATTCATCG GAATCATCCG TGGATGGATC TCACTATGGA TGCTAGCAGC
      721 AGCAGGAGTC CTAGGAATCG TCGGAATGTT CTGGCTAGCA CTAAAACGTT ACGGAATGAA
      781 CGTCTCAGGA GACGAAGCAT TCTACACCTT CCTATACCTA ACCCGTGACA CCTTCTCACC
      841 GTGGGAAAAC CTAGCACTAC TACTACAGAA CTACGACAAC ATCGACTTCC AGGGACTAGC
      901 ACCGATCGTC CGTGACTTCT ACGTCTTCAT CCCGTCATGG CTATGGCCGG GACGTCCGTC
      961 AATGGTCCTA AACTCAGCAA ACTACTTCAC CTGGGAAGTC CTAAACAACC ACTCAGGACT
     1021 AGCAATCTCA CCGACCCTAA TCGGATCACT AGTCGTCATG GGAGGAGCAC TATTCATCCC
     1081 GCTAGGAGCA ATCGTCGTCG GACTAATCAT CAAATGGTTC GACTGGCTAT ACGAACTAGG
     1141 AAACCGTGAA CCGAACCGTT ACAAAGCAGC AATCCTACAC TCATTCTGCT TCGGAGCAAT
     1201 CTTCAACATG ATCGTCCTAG CACGTGAAGG ACTAGACTCA TTCGTCTCAC GTGTCGTCTT
     1261 CTTCATCGTC GTCTTCGGAG CATGCCTAAT GATCGCAAAA CTACTATACT GGCTATTCGA
     1321 ATCAGCAGGA CTAATCCACA AACGTACCAA ATCATCACTA CGTACCCAGG TCGAAGGATG
     1381 AAGAGCTTCG AGACCACAGG GTATATTGAG TGCCCGTCCC CCGTTGACAA CGAAGGTAGG
     1441 GATAACAGGG TAATATAGAT GCTGCTGGGC GCAGGCGCTG AGGTGTCAAT CGTCGGAGCC
     1501 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1561 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1621 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1681 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1741 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1801 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1861 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1921 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1981 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     2041 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     2101 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     2161 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     2221 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     2281 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     2341 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     2401 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     2461 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     2521 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     2581 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     2641 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2701 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2761 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2821 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2881 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2941 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     3001 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     3061 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     3121 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     3181 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     3241 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     3301 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     3361 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     3421 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAG
//