LOCUS       Exported     3764 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3764
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1801..1848
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3696..3712
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3713..3744
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             100..3763
                     /label="ubiB"
                     /note="Is probably a protein kinase regulator of UbiI activity which is involved in aerobic coenzyme Q (ubiquinone) biosynthesis."
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWMPNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDGKLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDILPVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRRNFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFNRDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTARRFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAFKEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGATLVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     primer_bind     complement(1853..1869)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1936..2524)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2715..3575)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3576..3680)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1704..1763
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCTAAT GACCCCGGGA GAAGTCCGTC GTCTATACTT
       61 CATCATCCGT ACCTTCCTAT CATACGGACT AGACGAACTA ATCCCGAAAA TGCGTATCAC
      121 CCTACCGCTA CGTCTATGGC GTTACTCACT ATTCTGGATG CCGAACCGTC ACAAAGACAA
      181 ACTACTAGGA GAACGTCTAC GTCTAGCACT ACAGGAACTA GGACCGGTCT GGATCAAATT
      241 CGGACAGATG CTATCAACCC GTCGTGACCT ATTCCCGCCG CACATCGCAG ACCAGCTAGC
      301 ACTACTACAG GACAAAGTCG CACCGTTCGA CGGAAAACTA GCAAAACAGC AGATCGAAGC
      361 AGCAATGGGA GGACTACCGG TCGAAGCATG GTTCGACGAC TTCGAAATCA AACCGCTAGC
      421 ATCAGCATCA ATCGCACAGG TCCACACCGC ACGTCTAAAA TCAAACGGAA AAGAAGTCGT
      481 CATCAAAGTC ATCCGTCCGG ACATCCTACC GGTCATCAAA GCAGACCTAA AACTAATCTA
      541 CCGTCTAGCA CGTTGGGTCC CGCGTCTACT ACCGGACGGA CGTCGTCTAC GTCCGACCGA
      601 AGTCGTCCGT GAATACGAAA AAACCCTAAT CGACGAACTA AACCTACTAC GTGAATCAGC
      661 AAACGCAATC CAGCTACGTC GTAACTTCGA AGACTCACCG ATGCTATACA TCCCGGAAGT
      721 CTACCCGGAC TACTGCTCAG AAGGAATGAT GGTCATGGAA CGTATCTACG GAATCCCGGT
      781 CTCAGACGTC GCAGCACTAG AAAAAAACGG AACCAACATG AAACTACTAG CAGAACGTGG
      841 AGTCCAGGTC TTCTTCACCC AGGTCTTCCG TGACTCATTC TTCCACGCAG ACATGCACCC
      901 GGGAAACATC TTCGTCTCAT ACGAACACCC GGAAAACCCG AAATACATCG GAATCGACTG
      961 CGGAATCGTC GGATCACTAA ACAAAGAAGA CAAACGTTAC CTAGCAGAAA ACTTCATCGC
     1021 ATTCTTCAAC CGTGACTACC GTAAAGTCGC AGAACTACAC GTCGACTCAG GATGGGTCCC
     1081 GCCGGACACC AACGTCGAAG AATTCGAATT CGCAATCCGT ACCGTCTGCG AACCGATCTT
     1141 CGAAAAACCG CTAGCAGAAA TCTCATTCGG ACACGTCCTA CTAAACCTAT TCAACACCGC
     1201 ACGTCGTTTC AACATGGAAG TCCAGCCGCA GCTAGTCCTA CTACAGAAAA CCCTACTATA
     1261 CGTCGAAGGA GTCGGACGTC AGCTATACCC GCAGCTAGAC CTATGGAAAA CCGCAAAACC
     1321 GTTCCTAGAA TCATGGATCA AAGACCAGGT CGGAATCCCG GCACTAGTCC GTGCATTCAA
     1381 AGAAAAAGCA CCGTTCTGGG TCGAAAAAAT GCCGGAACTA CCGGAACTAG TCTACGACTC
     1441 ACTACGTCAG GGAAAATACC TACAGCACTC AGTCGACAAA ATCGCACGTG AACTACAGTC
     1501 AAACCACGTC CGTCAGGGAC AGTCACGTTA CTTCCTAGGA ATCGGAGCAA CCCTAGTCCT
     1561 ATCAGGAACC TTCCTACTAG TCTCACGTCC GGAATGGGGA CTAATGCCGG GATGGCTAAT
     1621 GGCAGGAGGA CTAATCGCAT GGTTCGTCGG ATGGCGTAAA ACCCGTTGAA GAGCTTCGAG
     1681 ACCAATGTCC TTAGGCAGTC GTCAAATTCG GGCTCAGCTA AAAATAGGGA TAACAGGGTA
     1741 ATCGGGCTTG GTAGCCTGAA TCGCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1801 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1861 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1921 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1981 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     2041 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     2101 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     2161 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     2221 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     2281 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     2341 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     2401 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     2461 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     2521 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     2581 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     2641 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     2701 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     2761 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     2821 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     2881 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     2941 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     3001 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     3061 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     3121 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     3181 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     3241 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     3301 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     3361 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     3421 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     3481 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     3541 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     3601 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     3661 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     3721 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAG
//