LOCUS Exported 6152 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..6152 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..6151 /note="Resistance to the antibiotics salinamide A, salinamide B, rifampicin, streptolydigin, CBR703, myxopyronin, and lipiarmycin can result from mutations in this protein." /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEIRSLGINIELEDE" /label="rpoB" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 6093..6152 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCTAA TGGTCTACTC 2041 ATACACCGAA AAAAAACGTA TCCGTAAAGA CTTCGGAAAA CGTCCGCAGG TCCTAGACGT 2101 CCCGTACCTA CTATCAATCC AGCTAGACTC ATTCCAGAAA TTCATCGAAC AGGACCCGGA 2161 AGGACAGTAC GGACTAGAAG CAGCATTCCG TTCAGTCTTC CCGATCCAGT CATACTCAGG 2221 AAACTCAGAA CTACAGTACG TCTCATACCG TCTAGGAGAA CCGGTCTTCG ACGTCCAGGA 2281 ATGCCAGATC CGTGGAGTCA CCTACTCAGC ACCGCTACGT GTCAAACTAC GTCTAGTCAT 2341 CTACGAACGT GAAGCACCGG AAGGAACCGT CAAAGACATC AAAGAACAGG AAGTCTACAT 2401 GGGAGAAATC CCGCTAATGA CCGACAACGG AACCTTCGTC ATCAACGGAA CCGAACGTGT 2461 CATCGTCTCA CAGCTACACC GTTCACCGGG AGTCTTCTTC GACTCAGACA AAGGAAAAAC 2521 CCACTCATCA GGAAAAGTCC TATACAACGC ACGTATCATC CCGTACCGTG GATCATGGCT 2581 AGACTTCGAA TTCGACCCGA AAGACAACCT ATTCGTCCGT ATCGACCGTC GTCGTAAACT 2641 ACCGGCAACC ATCATCCTAC GTGCACTAAA CTACACCACC GAACAGATCC TAGACCTATT 2701 CTTCGAAAAA GTCATCTTCG AAATCCGTGA CAACAAACTA CAGATGGAAC TAGTCCCGGA 2761 ACGTCTACGT GGAGAAACCG CATCATTCGA CATCGAAGCA AACGGAAAAG TCTACGTCGA 2821 AAAAGGACGT CGTATCACCG CACGTCACAT CCGTCAGCTA GAAAAAGACG ACGTCAAACT 2881 AATCGAAGTC CCGGTCGAAT ACATCGCAGG AAAAGTCGTC GCAAAAGACT ACATCGACGA 2941 ATCAACCGGA GAACTAATCT GCGCAGCAAA CATGGAACTA TCACTAGACC TACTAGCAAA 3001 ACTATCACAG TCAGGACACA AACGTATCGA AACCCTATTC ACCAACGACC TAGACCACGG 3061 ACCGTACATC TCAGAAACCC TACGTGTCGA CCCGACCAAC GACCGTCTAT CAGCACTAGT 3121 CGAAATCTAC CGTATGATGC GTCCGGGAGA ACCGCCGACC CGTGAAGCAG CAGAATCACT 3181 ATTCGAAAAC CTATTCTTCT CAGAAGACCG TTACGACCTA TCAGCAGTCG GACGTATGAA 3241 ATTCAACCGT TCACTACTAC GTGAAGAAAT CGAAGGATCA GGAATCCTAT CAAAAGACGA 3301 CATCATCGAC GTCATGAAAA AACTAATCGA CATCCGTAAC GGAAAAGGAG AAGTCGACGA 3361 CATCGACCAC CTAGGAAACC GTCGTATCCG TTCAGTCGGA GAAATGGCAG AAAACCAGTT 3421 CCGTGTCGGA CTAGTCCGTG TCGAACGTGC AGTCAAAGAA CGTCTATCAC TAGGAGACCT 3481 AGACACCCTA ATGCCGCAGG ACATGATCAA CGCAAAACCG ATCTCAGCAG CAGTCAAAGA 3541 ATTCTTCGGA TCATCACAGC TATCACAGTT CATGGACCAG AACAACCCGC TATCAGAAAT 3601 CACCCACAAA CGTCGTATCT CAGCACTAGG ACCGGGAGGA CTAACCCGTG AACGTGCAGG 3661 ATTCGAAGTC CGTGACGTCC ACCCGACCCA CTACGGACGT GTCTGCCCGA TCGAAACCCC 3721 GGAAGGACCG AACATCGGAC TAATCAACTC ACTATCAGTC TACGCACAGA CCAACGAATA 3781 CGGATTCCTA GAAACCCCGT ACCGTAAAGT CACCGACGGA GTCGTCACCG ACGAAATCCA 3841 CTACCTATCA GCAATCGAAG AAGGAAACTA CGTCATCGCA CAGGCAAACT CAAACCTAGA 3901 CGAAGAAGGA CACTTCGTCG AAGACCTAGT CACCTGCCGT TCAAAAGGAG AATCATCACT 3961 ATTCTCACGT GACCAGGTCG ACTACATGGA CGTCTCAACC CAGCAGGTCG TCTCAGTCGG 4021 AGCATCACTA ATCCCGTTCC TAGAACACGA CGACGCAAAC CGTGCACTAA TGGGAGCAAA 4081 CATGCAGCGT CAGGCAGTCC CGACCCTACG TGCAGACAAA CCGCTAGTCG GAACCGGAAT 4141 GGAACGTGCA GTCGCAGTCG ACTCAGGAGT CACCGCAGTC GCAAAACGTG GAGGAGTCGT 4201 CCAGTACGTC GACGCATCAC GTATCGTCAT CAAAGTCAAC GAAGACGAAA TGTACCCGGG 4261 AGAAGCAGGA ATCGACATCT ACAACCTAAC CAAATACACC CGTTCAAACC AGAACACCTG 4321 CATCAACCAG ATGCCGTGCG TCTCACTAGG AGAACCGGTC GAACGTGGAG ACGTCCTAGC 4381 AGACGGACCG TCAACCGACC TAGGAGAACT AGCACTAGGA CAGAACATGC GTGTCGCATT 4441 CATGCCGTGG AACGGATACA ACTTCGAAGA CTCAATCCTA GTCTCAGAAC GTGTCGTCCA 4501 GGAAGACCGT TTCACCACCA TCCACATCCA GGAACTAGCA TGCGTCTCAC GTGACACCAA 4561 ACTAGGACCG GAAGAAATCA CCGCAGACAT CCCGAACGTC GGAGAAGCAG CACTATCAAA 4621 ACTAGACGAA TCAGGAATCG TCTACATCGG AGCAGAAGTC ACCGGAGGAG ACATCCTAGT 4681 CGGAAAAGTC ACCCCGAAAG GAGAAACCCA GCTAACCCCG GAAGAAAAAC TACTACGTGC 4741 AATCTTCGGA GAAAAAGCAT CAGACGTCAA AGACTCATCA CTACGTGTCC CGAACGGAGT 4801 CTCAGGAACC GTCATCGACG TCCAGGTCTT CACCCGTGAC GGAGTCGAAA AAGACAAACG 4861 TGCACTAGAA ATCGAAGAAA TGCAGCTAAA ACAGGCAAAA AAAGACCTAT CAGAAGAACT 4921 ACAGATCCTA GAAGCAGGAC TATTCTCACG TATCCGTGCA GTCCTAGTCG CAGGAGGAGT 4981 CGAAGCAGAA AAACTAGACA AACTACCGCG TGACCGTTGG CTAGAACTAG GACTAACCGA 5041 CGAAGAAAAA CAGAACCAGC TAGAACAGCT AGCAGAACAG TACGACGAAC TAAAACACGA 5101 ATTCGAAAAA AAACTAGAAG CAAAACGTCG TAAAATCACC CAGGGAGACG ACCTAGCACC 5161 GGGAGTCCTA AAAATCGTCA AAGTCTACCT AGCAGTCAAA CGTCGTATCC AGCCGGGAGA 5221 CAAAATGGCA GGACGTCACG GAAACAAAGG AGTCATCTCA AAAATCAACC CGATCGAAGA 5281 CATGCCGTAC GACGAAAACG GAACCCCGGT CGACATCGTC CTAAACCCGC TAGGAGTCCC 5341 GTCACGTATG AACATCGGAC AGATCCTAGA AACCCACCTA GGAATGGCAG CAAAAGGAAT 5401 CGGAGACAAA ATCAACGCAA TGCTAAAACA GCAGCAGGAA GTCGCAAAAC TACGTGAATT 5461 CATCCAGCGT GCATACGACC TAGGAGCAGA CGTCCGTCAG AAAGTCGACC TATCAACCTT 5521 CTCAGACGAA GAAGTCATGC GTCTAGCAGA AAACCTACGT AAAGGAATGC CGATCGCAAC 5581 CCCGGTCTTC GACGGAGCAA AAGAAGCAGA AATCAAAGAA CTACTAAAAC TAGGAGACCT 5641 ACCGACCTCA GGACAGATCC GTCTATACGA CGGACGTACC GGAGAACAGT TCGAACGTCC 5701 GGTCACCGTC GGATACATGT ACATGCTAAA ACTAAACCAC CTAGTCGACG ACAAAATGCA 5761 CGCACGTTCA ACCGGATCAT ACTCACTAGT CACCCAGCAG CCGCTAGGAG GAAAAGCACA 5821 GTTCGGAGGA CAGCGTTTCG GAGAAATGGA AGTCTGGGCA CTAGAAGCAT ACGGAGCAGC 5881 ATACACCCTA CAGGAAATGC TAACCGTCAA ATCAGACGAC GTCAACGGAC GTACCAAAAT 5941 GTACAAAAAC ATCGTCGACG GAAACCACCA GATGGAACCG GGAATGCCGG AATCATTCAA 6001 CGTCCTACTA AAAGAAATCC GTTCACTAGG AATCAACATC GAACTAGAAG ACGAATGAAG 6061 AGCTTCGAGA CCATAGATCA TGTCGGCAGT CGTCTCCCTC TAGCAGCAAC AATTAGGGAT 6121 AACAGGGTAA TGTATTCGGA CTTCACGGTA TG //