LOCUS       Exported     4547 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4547
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      2584..2631
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4479..4495
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      4496..4527
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..4546
                     /note="Catalyzes the transfer of an acyl group from acyl-ACP to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme can utilize either acyl-CoA or acyl-ACP as the fatty acyl donor."
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIESATQGEG"
                     /label="plsB"
     primer_bind     complement(2636..2652)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(2719..3307)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3498..4358)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4359..4463)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2487..2546
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACCGGAGC CATACAGTAC GGTCTCTAAT GTCAGGATGG CCGCGTATCT ACTACAAACT
       61 ACTAAACCTA CCGCTATCAA TCCTAGTCAA ATCAAAATCA ATCCCGGCAG ACCCGGCACC
      121 GGAACTAGGA CTAGACACCT CACGTCCGAT CATGTACGTC CTACCGTACA ACTCAAAAGC
      181 AGACCTACTA ACCCTACGTG CACAGTGCCT AGCACACGAC CTACCGGACC CGCTAGAACC
      241 GCTAGAAATC GACGGAACCC TACTACCGCG TTACGTCTTC ATCCACGGAG GACCGCGTGT
      301 CTTCACCTAC TACACCCCGA AAGAAGAATC AATCAAACTA TTCCACGACT ACCTAGACCT
      361 ACACCGTTCA AACCCGAACC TAGACGTCCA GATGGTCCCG GTCTCAGTCA TGTTCGGACG
      421 TGCACCGGGA CGTGAAAAAG GAGAAGTCAA CCCGCCGCTA CGTATGCTAA ACGGAGTCCA
      481 GAAATTCTTC GCAGTCCTAT GGCTAGGACG TGACTCATTC GTCCGTTTCT CACCGTCAGT
      541 CTCACTACGT CGTATGGCAG ACGAACACGG AACCGACAAA ACCATCGCAC AGAAACTAGC
      601 ACGTGTCGCA CGTATGCACT TCGCACGTCA GCGTCTAGCA GCAGTCGGAC CGCGTCTACC
      661 GGCACGTCAG GACCTATTCA ACAAACTACT AGCATCACGT GCAATCGCAA AAGCAGTCGA
      721 AGACGAAGCA CGTTCAAAAA AAATCTCACA CGAAAAAGCA CAGCAGAACG CAATCGCACT
      781 AATGGAAGAA ATCGCAGCAA ACTTCTCATA CGAAATGATC CGTCTAACCG ACCGTATCCT
      841 AGGATTCACC TGGAACCGTC TATACCAGGG AATCAACGTC CACAACGCAG AACGTGTCCG
      901 TCAGCTAGCA CACGACGGAC ACGAACTAGT CTACGTCCCG TGCCACCGTT CACACATGGA
      961 CTACCTACTA CTATCATACG TCCTATACCA CCAGGGACTA GTCCCGCCGC ACATCGCAGC
     1021 AGGAATCAAC CTAAACTTCT GGCCGGCAGG ACCGATCTTC CGTCGTCTAG GAGCATTCTT
     1081 CATCCGTCGT ACCTTCAAAG GAAACAAACT ATACTCAACC GTCTTCCGTG AATACCTAGG
     1141 AGAACTATTC TCACGTGGAT ACTCAGTCGA ATACTTCGTC GAAGGAGGAC GTTCACGTAC
     1201 CGGACGTCTA CTAGACCCGA AAACCGGAAC CCTATCAATG ACCATCCAGG CAATGCTACG
     1261 TGGAGGAACC CGTCCGATCA CCCTAATCCC GATCTACATC GGATACGAAC ACGTCATGGA
     1321 AGTCGGAACC TACGCAAAAG AACTACGTGG AGCAACCAAA GAAAAAGAAT CACTACCGCA
     1381 GATGCTACGT GGACTATCAA AACTACGTAA CCTAGGACAG GGATACGTCA ACTTCGGAGA
     1441 ACCGATGCCG CTAATGACCT ACCTAAACCA GCACGTCCCG GACTGGCGTG AATCAATCGA
     1501 CCCGATCGAA GCAGTCCGTC CGGCATGGCT AACCCCGACC GTCAACAACA TCGCAGCAGA
     1561 CCTAATGGTC CGTATCAACA ACGCAGGAGC AGCAAACGCA ATGAACCTAT GCTGCACCGC
     1621 ACTACTAGCA TCACGTCAGC GTTCACTAAC CCGTGAACAG CTAACCGAAC AGCTAAACTG
     1681 CTACCTAGAC CTAATGCGTA ACGTCCCGTA CTCAACCGAC TCAACCGTCC CGTCAGCATC
     1741 AGCATCAGAA CTAATCGACC ACGCACTACA GATGAACAAA TTCGAAGTCG AAAAAGACAC
     1801 CATCGGAGAC ATCATCATCC TACCGCGTGA ACAGGCAGTC CTAATGACCT ACTACCGTAA
     1861 CAACATCGCA CACATGCTAG TCCTACCGTC ACTAATGGCA GCAATCGTCA CCCAGCACCG
     1921 TCACATCTCA CGTGACGTCC TAATGGAACA CGTCAACGTC CTATACCCGA TGCTAAAAGC
     1981 AGAACTATTC CTACGTTGGG ACCGTGACGA ACTACCGGAC GTCATCGACG CACTAGCAAA
     2041 CGAAATGCAG CGTCAGGGAC TAATCACCCT ACAGGACGAC GAACTACACA TCAACCCGGC
     2101 ACACTCACGT ACCCTACAGC TACTAGCAGC AGGAGCACGT GAAACCCTAC AGCGTTACGC
     2161 AATCACCTTC TGGCTACTAT CAGCAAACCC GTCAATCAAC CGTGGAACCC TAGAAAAAGA
     2221 ATCACGTACC GTCGCACAGC GTCTATCAGT CCTACACGGA ATCAACGCAC CGGAATTCTT
     2281 CGACAAAGCA GTCTTCTCAT CACTAGTCCT AACCCTACGT GACGAAGGAT ACATCTCAGA
     2341 CTCAGGAGAC GCAGAACCGG CAGAAACCAT GAAAGTCTAC CAGCTACTAG CAGAACTAAT
     2401 CACCTCAGAC GTCCGTCTAA CCATCGAATC AGCAACCCAG GGAGAAGGAT GAAGAGCTTC
     2461 GAGACCAGAG AGGCATGATT GACCTCGGTG CCAAGTGAGT ATGGACGTAG GGATAACAGG
     2521 GTAATGATAC CCCTAGGGCC ACGGCACGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
     2581 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     2641 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     2701 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     2761 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     2821 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     2881 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     2941 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     3001 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     3061 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     3121 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     3181 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     3241 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     3301 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     3361 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     3421 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     3481 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     3541 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     3601 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     3661 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     3721 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     3781 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     3841 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     3901 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     3961 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     4021 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     4081 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     4141 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     4201 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     4261 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     4321 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     4381 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     4441 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     4501 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAG
//