LOCUS       Exported     3539 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3539
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3445
                     /label="dnaB"
                     /note="Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins."
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    3480..3539
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCTAA TGGCAGGAAA
     2041 CAAACCGTTC AACAAACAGC AGGCAGAACC GCGTGAACGT GACCCGCAGG TCGCAGGACT
     2101 AAAAGTCCCG CCGCACTCAA TCGAAGCAGA ACAGTCAGTC CTAGGAGGAC TAATGCTAGA
     2161 CAACGAACGT TGGGACGACG TCGCAGAACG TGTCGTCGCA GACGACTTCT ACACCCGTCC
     2221 GCACCGTCAC ATCTTCACCG AAATGGCACG TCTACAGGAA TCAGGATCAC CGATCGACCT
     2281 AATCACCCTA GCAGAATCAC TAGAACGTCA GGGACAGCTA GACTCAGTCG GAGGATTCGC
     2341 ATACCTAGCA GAACTATCAA AAAACACCCC GTCAGCAGCA AACATCTCAG CATACGCAGA
     2401 CATCGTCCGT GAACGTGCAG TCGTCCGTGA AATGATCTCA GTCGCAAACG AAATCGCAGA
     2461 AGCAGGATTC GACCCGCAGG GACGTACCTC AGAAGACCTA CTAGACCTAG CAGAATCACG
     2521 TGTCTTCAAA ATCGCAGAAT CACGTGCAAA CAAAGACGAA GGACCGAAAA ACATCGCAGA
     2581 CGTCCTAGAC GCAACCGTCG CACGTATCGA ACAGCTATTC CAGCAGCCGC ACGACGGAGT
     2641 CACCGGAGTC AACACCGGAT ACGACGACCT AAACAAAAAA ACCGCAGGAC TACAGCCGTC
     2701 AGACCTAATC ATCGTCGCAG CACGTCCGTC AATGGGAAAA ACCACCTTCG CAATGAACCT
     2761 AGTCGAAAAC GCAGCAATGC TACAGGACAA ACCGGTCCTA ATCTTCTCAC TAGAAATGCC
     2821 GTCAGAACAG ATCATGATGC GTTCACTAGC ATCACTATCA CGTGTCGACC AGACCAAAAT
     2881 CCGTACCGGA CAGCTAGACG ACGAAGACTG GGCACGTATC TCAGGAACCA TGGGAATCCT
     2941 ACTAGAAAAA CGTAACATCT ACATCGACGA CTCATCAGGA CTAACCCCGA CCGAAGTCCG
     3001 TTCACGTGCA CGTCGTATCG CACGTGAACA CGGAGGAATC GGACTAATCA TGATCGACTA
     3061 CCTACAGCTA ATGCGTGTCC CGGCACTATC AGACAACCGT ACCCTAGAAA TCGCAGAAAT
     3121 CTCACGTTCA CTAAAAGCAC TAGCAAAAGA ACTAAACGTC CCGGTCGTCG CACTATCACA
     3181 GCTAAACCGT TCACTAGAAC AGCGTGCAGA CAAACGTCCG GTCAACTCAG ACCTACGTGA
     3241 ATCAGGATCA ATCGAACAGG ACGCAGACCT AATCATGTTC ATCTACCGTG ACGAAGTCTA
     3301 CCACGAAAAC TCAGACCTAA AAGGAATCGC AGAAATCATC ATCGGAAAAC AGCGTAACGG
     3361 ACCGATCGGA ACCGTCCGTC TAACCTTCAA CGGACAGTGG TCACGTTTCG ACAACTACGC
     3421 AGGACCGCAG TACGACGACG AATGAAGAGC TTCGAGACCA GTATTGCCGG ACTAAACCCC
     3481 GGACTATAAT TAATTGTGGA TAGGGATAAC AGGGTAATGC TTACGGCGAT TATTTAAAA
//