LOCUS       Exported     4979 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4979
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      3016..3063
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4911..4927
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4928..4959
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..4978
                     /label="valS"
                     /note="Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner."
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNVTGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTRHDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRLYKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLVVATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCVKITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQKLERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKPAVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRNEDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSGFDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPLDMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALASTGRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFNQTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLVTVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTEWLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVLPADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEGFVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     primer_bind     complement(3068..3084)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(3151..3739)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3930..4790)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4791..4895)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2919..2978
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACCGGAGC CATACAGTAC GGTCTCTAAT GGAAAAAACC TACAACCCGC AGGACATCGA
       61 ACAGCCGCTA TACGAACACT GGGAAAAACA GGGATACTTC AAACCGAACG GAGACGAATC
      121 ACAGGAATCA TTCTGCATCA TGATCCCGCC GCCGAACGTC ACCGGATCAC TACACATGGG
      181 ACACGCATTC CAGCAGACCA TCATGGACAC CATGATCCGT TACCAGCGTA TGCAGGGAAA
      241 AAACACCCTA TGGCAGGTCG GAACCGACCA CGCAGGAATC GCAACCCAGA TGGTCGTCGA
      301 ACGTAAAATC GCAGCAGAAG AAGGAAAAAC CCGTCACGAC TACGGACGTG AAGCATTCAT
      361 CGACAAAATC TGGGAATGGA AAGCAGAATC AGGAGGAACC ATCACCCGTC AGATGCGTCG
      421 TCTAGGAAAC TCAGTCGACT GGGAACGTGA ACGTTTCACC ATGGACGAAG GACTATCAAA
      481 CGCAGTCAAA GAAGTCTTCG TCCGTCTATA CAAAGAAGAC CTAATCTACC GTGGAAAACG
      541 TCTAGTCAAC TGGGACCCGA AACTACGTAC CGCAATCTCA GACCTAGAAG TCGAAAACCG
      601 TGAATCAAAA GGATCAATGT GGCACATCCG TTACCCGCTA GCAGACGGAG CAAAAACCGC
      661 AGACGGAAAA GACTACCTAG TCGTCGCAAC CACCCGTCCG GAAACCCTAC TAGGAGACAC
      721 CGGAGTCGCA GTCAACCCGG AAGACCCGCG TTACAAAGAC CTAATCGGAA AATACGTCAT
      781 CCTACCGCTA GTCAACCGTC GTATCCCGAT CGTCGGAGAC GAACACGCAG ACATGGAAAA
      841 AGGAACCGGA TGCGTCAAAA TCACCCCGGC ACACGACTTC AACGACTACG AAGTCGGAAA
      901 ACGTCACGCA CTACCGATGA TCAACATCCT AACCTTCGAC GGAGACATCC GTGAATCAGC
      961 ACAGGTCTTC GACACCAAAG GAAACGAATC AGACGTCTAC TCATCAGAAA TCCCGGCAGA
     1021 ATTCCAGAAA CTAGAACGTT TCGCAGCACG TAAAGCAGTC GTCGCAGCAG TCGACGCACT
     1081 AGGACTACTA GAAGAAATCA AACCGCACGA CCTAACCGTC CCGTACGGAG ACCGTGGAGG
     1141 AGTCGTCATC GAACCGATGC TAACCGACCA GTGGTACGTC CGTGCAGACG TCCTAGCAAA
     1201 ACCGGCAGTC GAAGCAGTCG AAAACGGAGA CATCCAGTTC GTCCCGAAAC AGTACGAAAA
     1261 CATGTACTTC TCATGGATGC GTGACATCCA GGACTGGTGC ATCTCACGTC AGCTATGGTG
     1321 GGGACACCGT ATCCCGGCAT GGTACGACGA AGCAGGAAAC GTCTACGTCG GACGTAACGA
     1381 AGACGAAGTC CGTAAAGAAA ACAACCTAGG AGCAGACGTC GTCCTACGTC AGGACGAAGA
     1441 CGTCCTAGAC ACCTGGTTCT CATCAGCACT ATGGACCTTC TCAACCCTAG GATGGCCGGA
     1501 AAACACCGAC GCACTACGTC AGTTCCACCC GACCTCAGTC ATGGTCTCAG GATTCGACAT
     1561 CATCTTCTTC TGGATCGCAC GTATGATCAT GATGACCATG CACTTCATCA AAGACGAAAA
     1621 CGGAAAACCG CAGGTCCCGT TCCACACCGT CTACATGACC GGACTAATCC GTGACGACGA
     1681 AGGACAGAAA ATGTCAAAAT CAAAAGGAAA CGTCATCGAC CCGCTAGACA TGGTCGACGG
     1741 AATCTCACTA CCGGAACTAC TAGAAAAACG TACCGGAAAC ATGATGCAGC CGCAGCTAGC
     1801 AGACAAAATC CGTAAACGTA CCGAAAAACA GTTCCCGAAC GGAATCGAAC CGCACGGAAC
     1861 CGACGCACTA CGTTTCACCC TAGCAGCACT AGCATCAACC GGACGTGACA TCAACTGGGA
     1921 CATGAAACGT CTAGAAGGAT ACCGTAACTT CTGCAACAAA CTATGGAACG CATCACGTTT
     1981 CGTCCTAATG AACACCGAAG GACAGGACTG CGGATTCAAC GGAGGAGAAA TGACCCTATC
     2041 ACTAGCAGAC CGTTGGATCC TAGCAGAATT CAACCAGACC ATCAAAGCAT ACCGTGAAGC
     2101 ACTAGACTCA TTCCGTTTCG ACATCGCAGC AGGAATCCTA TACGAATTCA CCTGGAACCA
     2161 GTTCTGCGAC TGGTACCTAG AACTAACCAA ACCGGTCATG AACGGAGGAA CCGAAGCAGA
     2221 ACTACGTGGA ACCCGTCACA CCCTAGTCAC CGTCCTAGAA GGACTACTAC GTCTAGCACA
     2281 CCCGATCATC CCGTTCATCA CCGAAACCAT CTGGCAGCGT GTCAAAGTCC TATGCGGAAT
     2341 CACCGCAGAC ACCATCATGC TACAGCCGTT CCCGCAGTAC GACGCATCAC AGGTCGACGA
     2401 AGCAGCACTA GCAGACACCG AATGGCTAAA ACAGGCAATC GTCGCAGTCC GTAACATCCG
     2461 TGCAGAAATG AACATCGCAC CGGGAAAACC GCTAGAACTA CTACTACGTG GATGCTCAGC
     2521 AGACGCAGAA CGTCGTGTCA ACGAAAACCG TGGATTCCTA CAGACCCTAG CACGTCTAGA
     2581 ATCAATCACC GTCCTACCGG CAGACGACAA AGGACCGGTC TCAGTCACCA AAATCATCGA
     2641 CGGAGCAGAA CTACTAATCC CGATGGCAGG ACTAATCAAC AAAGAAGACG AACTAGCACG
     2701 TCTAGCAAAA GAAGTCGCAA AAATCGAAGG AGAAATCTCA CGTATCGAAA ACAAACTAGC
     2761 AAACGAAGGA TTCGTCGCAC GTGCACCGGA AGCAGTCATC GCAAAAGAAC GTGAAAAACT
     2821 AGAAGGATAC GCAGAAGCAA AAGCAAAACT AATCGAACAG CAGGCAGTCA TCGCAGCACT
     2881 ATGAAGAGCT TCGAGACCAA TATCACGCAA AAGCACCGCG TCACTCAGCT TTTGCGAACT
     2941 AGGGATAACA GGGTAATGAG GGGTTAACTG TACGCGACCG CTGAGGTGTC AATCGTCGGA
     3001 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     3061 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     3121 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     3181 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     3241 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     3301 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     3361 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     3421 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     3481 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     3541 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     3601 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     3661 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     3721 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     3781 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     3841 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     3901 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     3961 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     4021 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     4081 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     4141 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     4201 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     4261 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     4321 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     4381 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     4441 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     4501 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     4561 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     4621 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     4681 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     4741 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     4801 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     4861 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     4921 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//