LOCUS Exported 3971 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3971 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3937 /label="Mfl006" /note="A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner." /translation="MAEQNYGAESIKVLKGLEAVRKRPGMYIGSTSKAGLHHLVWEIMDNSVDEAMAGFANKIILTITKEGEIIVQDNGRGIPVGIQADSGKSALELVFTQLHAGGKFDSDSYKISGGLHGVGASVVNALSLYVDVQVKREGKIHHQVFSGGGTQQTEIEIIGETNETGTIVRFKPDPEIFLEGTEFDYETIRNKVKQLSYLNKGLVIELNDLRIDKHVEYHFPNGILDYVKEKNETKVKINPSIFYVDDKHDDIEVEVALQYNAEYQENLITFVNNINTHEGGTHEDGLRQSLVRVINRYAEKVATGNKPAAKYSWDDIKEGMVCIVSIRHTDPQYEGQTKTKLANPDAKKAVDAVVGDAFEEFLLKSPEDAKAIIDKNANAQKARIAAQRAREETRRKSALDTFSLPGKLADCESKDPEIAELYLVEGDSAGGSAKTGRNRRFQAILPLRGKVLNVERVAEVRAFANNEIKSIVTAIGTGIKEDIDLSKLRYGKIVIMTDADVDGAHIRTLLLTFFYRYMKQLVVDGHVYIAQPPLYKIEAGKKVAYAYSDVELEELKAGDFKDSRFTIQRYKGLGEMDPIQLWETTMDPEKRTMLQIKLEDAAIANEVFSDLMGEDPELRRNYIQENAEFVENIDF" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAAGGGGCC GTCAATATCA GGGTCTCAAA TGGCGGAGCA 2041 GAATTACGGT GCCGAAAGCA TTAAAGTTTT GAAAGGTCTG GAGGCAGTCC GTAAACGCCC 2101 AGGCATGTAC ATCGGCTCTA CCAGCAAAGC GGGTCTGCAC CACTTGGTCT GGGAGATCAT 2161 GGATAATAGC GTTGACGAGG CTATGGCCGG CTTTGCGAAT AAAATCATTC TGACCATCAC 2221 GAAGGAAGGT GAGATTATCG TACAAGACAA TGGTCGCGGT ATTCCGGTCG GTATTCAGGC 2281 GGACAGCGGC AAAAGCGCAT TGGAGCTGGT CTTTACCCAG CTGCACGCGG GTGGCAAATT 2341 CGATAGCGAT TCTTATAAGA TCAGCGGTGG TCTGCATGGT GTTGGCGCGA GCGTTGTTAA 2401 TGCACTGAGC CTGTATGTTG ACGTTCAAGT GAAGCGCGAG GGTAAGATTC ACCACCAGGT 2461 GTTTAGCGGT GGTGGTACGC AACAGACGGA GATTGAAATT ATTGGCGAAA CCAATGAAAC 2521 GGGTACGATT GTGCGTTTCA AACCGGATCC AGAGATCTTT CTGGAGGGTA CCGAGTTCGA 2581 CTACGAAACC ATCCGTAATA AGGTGAAACA GTTGAGCTAC TTAAATAAGG GTCTGGTTAT 2641 CGAGCTGAAT GACTTACGTA TTGATAAGCA CGTTGAGTAT CATTTTCCGA ATGGCATTCT 2701 GGATTACGTG AAAGAGAAAA ATGAAACGAA GGTGAAGATC AACCCGAGCA TTTTCTACGT 2761 TGACGATAAA CATGACGACA TCGAAGTCGA GGTGGCACTG CAGTACAACG CCGAATACCA 2821 GGAGAACCTG ATTACGTTCG TCAATAATAT TAATACGCAC GAAGGTGGCA CCCATGAGGA 2881 CGGTTTGCGT CAAAGCCTGG TCCGTGTCAT CAACCGCTAC GCCGAGAAGG TGGCGACCGG 2941 CAATAAGCCG GCGGCAAAAT ATTCGTGGGA TGATATCAAA GAGGGTATGG TTTGCATTGT 3001 TAGCATCCGT CATACCGACC CGCAGTACGA AGGTCAGACC AAGACCAAAC TGGCGAATCC 3061 AGATGCGAAA AAAGCTGTTG ATGCGGTTGT GGGTGATGCG TTCGAGGAGT TTCTGCTTAA 3121 GAGCCCGGAA GATGCCAAAG CGATTATCGA TAAGAACGCG AACGCCCAGA AAGCGCGCAT 3181 TGCGGCGCAA CGCGCCCGTG AGGAAACCCG TCGTAAGAGC GCACTGGATA CGTTCAGCCT 3241 GCCGGGTAAG CTGGCGGACT GTGAGTCCAA AGATCCGGAG ATCGCAGAGC TGTATCTGGT 3301 TGAGGGTGAT AGCGCGGGTG GTAGCGCAAA AACCGGTCGT AACCGTCGTT TTCAGGCGAT 3361 TCTGCCACTG CGTGGTAAGG TGCTGAACGT TGAGCGTGTG GCAGAGGTCC GTGCGTTTGC 3421 GAATAATGAA ATTAAAAGCA TCGTCACCGC GATCGGTACC GGTATCAAAG AAGATATCGA 3481 TCTGTCGAAA CTGCGCTATG GTAAAATTGT GATCATGACC GATGCGGATG TGGATGGTGC 3541 GCATATCCGT ACCTTGCTGC TGACGTTCTT CTACCGTTAC ATGAAACAGT TGGTTGTCGA 3601 CGGTCACGTG TACATTGCGC AACCGCCACT GTACAAAATC GAGGCTGGTA AGAAGGTGGC 3661 CTACGCCTAC AGCGACGTGG AGTTGGAGGA GCTGAAAGCG GGTGATTTTA AGGATAGCCG 3721 CTTTACGATT CAACGTTACA AAGGTCTGGG TGAGATGGAC CCAATCCAAC TGTGGGAAAC 3781 TACCATGGAT CCGGAAAAAC GTACTATGCT GCAAATTAAG CTGGAGGACG CGGCGATCGC 3841 GAATGAAGTG TTCTCCGACC TGATGGGCGA GGACCCGGAA CTGCGTCGCA ACTACATCCA 3901 GGAGAATGCA GAGTTCGTTG AAAATATCGA CTTTTGAAGA GCTTAGAGAC CGCAGCGATT 3961 CGGATACTAC T //