LOCUS       Exported     3527 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3527
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3493
                     /label="Mfl009"
                     /note=""
                     /translation="MKKLNFPKSFLWGGATSAAQIEGAWNIDGKSLTLPEIQPFIELKDKSDLSKLHNERNIIFKNALEGKFEGHYPKRFGIDFYHRYKEDIALFKEAGMNIFRMSISWARIFPNAFDEKPNLNGLKFYRDVFEECKKNNMEIMVTMSHFDYPFELMKSNPKGWLDPKVKELFLKYAKTILDEYADIVKYWLPFNEINIGAFAVEAGLGIMPDSKRTEAETKELSIKGLHNLFVAQAEIVKYAKKFKNIKLGCMMADMTTYSLDCNPVNVLANMKREQILKFMFYDVMIKGKYPGYFKNIVKTLTNKEITFDENELKLLKENPLEFISFSYYMSNTVSTDPNQEKTQGNLTSAGKNPFLKATDWGWQIDPIGLRYVLNQLWDRYEVPLFISENGIGVLETLNEENAVNDDYRIDYLSKHIEQIDLALKDGVDVFGYTMWTPIDLVSASTCEMAKRYGLIFVDYDDYHNGTGNRYPKKSFNWFKEFIKTKEI"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATATCACG CAAAAGCACC GGGTCTCAAA TGAAAAAGCT
     2041 GAACTTCCCA AAGAGCTTCT TGTGGGGCGG CGCAACCAGC GCCGCGCAGA TCGAGGGTGC
     2101 GTGGAACATC GACGGTAAAA GCCTGACTCT GCCTGAAATT CAACCGTTCA TTGAACTGAA
     2161 AGACAAAAGC GACCTGTCCA AGCTGCATAA CGAACGTAAC ATTATCTTTA AAAATGCGTT
     2221 GGAGGGCAAG TTTGAAGGTC ATTACCCGAA ACGTTTTGGT ATCGATTTTT ATCACCGCTA
     2281 CAAAGAGGAT ATCGCCCTGT TCAAGGAAGC GGGCATGAAT ATCTTTCGTA TGTCCATTAG
     2341 CTGGGCTCGT ATTTTCCCGA ACGCTTTTGA TGAGAAACCT AATCTGAATG GCCTGAAATT
     2401 TTACCGTGAC GTTTTCGAAG AGTGCAAAAA GAACAATATG GAGATCATGG TTACCATGAG
     2461 CCATTTTGAC TACCCGTTCG AACTGATGAA AAGCAATCCG AAGGGCTGGC TTGACCCAAA
     2521 AGTTAAGGAG CTGTTTCTGA AGTATGCTAA GACCATTTTG GACGAGTATG CGGACATCGT
     2581 AAAGTACTGG CTGCCGTTTA ACGAGATCAA TATCGGTGCC TTCGCAGTGG AGGCCGGCCT
     2641 GGGTATCATG CCGGATAGCA AGCGCACGGA AGCGGAAACC AAGGAGCTGA GCATCAAGGG
     2701 CCTGCACAAC TTGTTTGTCG CACAGGCAGA GATCGTTAAG TATGCAAAGA AATTTAAAAA
     2761 CATTAAGCTG GGTTGCATGA TGGCCGACAT GACGACCTAT AGCCTGGACT GCAATCCGGT
     2821 TAATGTCCTG GCGAATATGA AGCGTGAGCA AATCCTGAAA TTCATGTTTT ACGATGTTAT
     2881 GATTAAGGGC AAGTATCCAG GTTACTTTAA GAATATCGTT AAAACCCTGA CCAATAAAGA
     2941 AATCACTTTC GATGAGAATG AACTGAAGCT CCTGAAGGAA AACCCGCTGG AGTTTATTAG
     3001 CTTCAGCTAC TATATGAGCA ATACCGTCAG CACCGATCCG AACCAGGAGA AAACCCAGGG
     3061 CAACCTGACC AGCGCGGGTA AAAACCCGTT TCTGAAAGCA ACCGACTGGG GCTGGCAGAT
     3121 TGATCCGATT GGCCTGCGTT ACGTCCTGAA CCAATTGTGG GATCGCTACG AAGTTCCGCT
     3181 GTTCATCTCC GAAAACGGCA TTGGTGTGCT GGAAACCCTG AACGAGGAGA ACGCAGTGAA
     3241 CGATGACTAC CGCATCGATT ATTTGTCGAA GCACATTGAG CAGATCGACT TGGCGCTGAA
     3301 AGATGGTGTC GATGTTTTCG GCTATACCAT GTGGACCCCG ATTGATCTGG TCAGCGCATC
     3361 TACGTGCGAA ATGGCTAAGC GTTACGGTTT GATCTTTGTG GATTATGACG ATTATCATAA
     3421 TGGTACCGGC AATCGTTATC CGAAAAAATC GTTCAATTGG TTCAAGGAAT TCATCAAGAC
     3481 CAAAGAAATT TGAAGAGCTT AGAGACCCCG GTTGTACCTA TCGAGTG
//