LOCUS Exported 3518 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3518 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3484 /label="Mfl012" /note="" /translation="MTKKLEFPSNFLWGGATAASQIEGAYNVDGKSLSAIEMIPFIELKDRKDLSQTRKLTTQDLLDSIENKKGLHYPKRFGIDFYHKYKEDIALFKEAGMKVFRMSVAWTRIFPNGDETEPNRKGLEFYKNVFEECKKQGLEIMVTINHFDMPFSIIQKYGGWKNRQVIDMYFKYSKTLIDEFHSYVRYWLPFNEINLGVLFSAMGLEGNNEEKSLAERLAGTYQDLHNQFVAQAKFIEYSKKFSNTKVGCMVADTTTYAIDCNPVNVLENQRKEQMKKFFYYDVMTKGEYPSYSKRYFKENGINIVKEKTDDELLKNNTVEFLSFSYYMSSTIAKEEVELTEGNLMKMGKNPFLVATEWGWQIDPIGLRYCLNQLWDRYHLPLFISENGMGVLETLNENNTVDDDYRIEYLSRHFEQMSEAIKDGVNLFGYTMWTPIDVVSAGSNEMSKRYGLIFVDYDDYHRGTGNRFKKKSFTWFQNFMKTSEV" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAGGAACTA TGGCATCGAG CGGTCTCAAA TGACGAAGAA 2041 GCTGGAATTC CCGAGCAATT TTCTGTGGGG TGGTGCAACC GCGGCAAGCC AGATCGAGGG 2101 TGCATATAAC GTTGATGGTA AGTCGCTGTC CGCCATCGAA ATGATTCCGT TCATCGAATT 2161 AAAAGACCGC AAAGACCTGA GCCAGACCCG CAAACTGACC ACCCAGGATC TGTTGGATAG 2221 CATCGAGAAC AAAAAAGGCC TGCACTATCC GAAGCGCTTC GGCATCGATT TCTACCATAA 2281 ATACAAGGAG GATATCGCCC TGTTTAAAGA AGCGGGCATG AAAGTTTTCC GTATGAGCGT 2341 TGCGTGGACG CGTATCTTCC CGAACGGTGA TGAAACTGAG CCGAATCGTA AAGGCTTAGA 2401 ATTTTACAAG AATGTCTTTG AAGAGTGTAA GAAGCAAGGT CTGGAGATCA TGGTGACGAT 2461 CAATCATTTT GATATGCCGT TTTCCATCAT CCAGAAGTAT GGTGGTTGGA AAAACCGCCA 2521 GGTAATCGAT ATGTATTTCA AGTATTCCAA AACCCTCATC GATGAATTTC ATTCCTATGT 2581 GCGTTACTGG TTGCCGTTCA ATGAGATTAA TCTGGGCGTG CTGTTCTCCG CCATGGGCCT 2641 GGAGGGTAAC AATGAGGAAA AGAGCCTGGC TGAGCGTCTG GCGGGTACGT ACCAAGATCT 2701 GCATAACCAA TTCGTCGCGC AGGCCAAGTT CATCGAGTAT AGCAAGAAGT TCTCTAATAC 2761 GAAAGTCGGT TGCATGGTCG CGGACACCAC CACGTACGCG ATTGATTGCA ATCCGGTTAA 2821 CGTCCTGGAG AACCAGCGTA AGGAGCAGAT GAAAAAGTTC TTTTATTATG ACGTCATGAC 2881 TAAGGGTGAA TACCCGAGCT ATAGCAAGCG TTACTTCAAA GAGAATGGTA TCAATATCGT 2941 TAAAGAGAAA ACGGATGACG AGCTGCTGAA AAATAACACG GTGGAATTCC TGAGCTTCTC 3001 TTACTACATG TCCAGCACGA TCGCGAAGGA AGAAGTGGAA CTGACCGAGG GTAATCTGAT 3061 GAAAATGGGT AAAAACCCGT TCCTGGTCGC GACTGAATGG GGTTGGCAGA TTGATCCGAT 3121 CGGCCTGCGT TATTGCTTGA ATCAGCTGTG GGATCGTTAC CATCTGCCGC TGTTCATTAG 3181 CGAAAATGGT ATGGGTGTGC TGGAAACGCT GAATGAGAAC AATACGGTGG ACGACGACTA 3241 TAGAATTGAA TACCTGAGCC GCCATTTTGA ACAAATGAGC GAAGCGATTA AAGATGGTGT 3301 TAACCTGTTC GGCTATACCA TGTGGACGCC TATTGATGTG GTCAGCGCCG GTAGCAACGA 3361 AATGAGCAAG CGCTATGGCC TGATTTTCGT GGACTACGAC GATTATCACC GTGGTACTGG 3421 CAATCGTTTT AAGAAAAAGA GCTTCACCTG GTTCCAAAAT TTCATGAAAA CCTCTGAAGT 3481 GTGAAGAGCT TAGAGACCGG CTCGATACAG TTTCACGT //