LOCUS Exported 2980 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2980 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1018..1065 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 2913..2929 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2930..2961 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..946 /translation="MKYYTFDIGGTSTKYLIFEDKKVIKSGVIEYKEQMKEKGSIRDKITLTKIFDEISVMLENESMDFCLGLSIPGIVDSKNQKILSDSGISETNIDIQDYFKKFKNIIKLSIENDAKSAAYGEFYFGQDQKPINMVHLTFGTGLGCGIIINKEIYRTINHKAGEIGKSYSNINSTESVVVMDTSVIAILIKYKMATGKEISGKEFFELYEKKDPLVQKLMKDWTSAVARTIIGLDYSFDFDLLTIGGGVSDNKIFQKLLFEELDLNKDFEFMGNKLSSNGQTISKVKISNLKNDAGCYGMLAILKQN" /label="Mfl013" /note="" primer_bind complement(1070..1086) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1153..1741) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1932..2792) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2793..2897) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 ACAGAACGAA CAGGCACTAC GGTCTCAAAT GAAATACTAT ACCTTTGACA TTGGCGGTAC 61 TTCTACCAAA TACTTGATCT TCGAGGACAA AAAGGTCATC AAAAGCGGTG TCATCGAATA 121 CAAGGAACAA ATGAAGGAAA AGGGTAGCAT CCGCGATAAA ATTACCCTGA CGAAGATTTT 181 CGACGAGATC AGCGTCATGC TGGAAAACGA AAGCATGGAC TTTTGCCTGG GTTTGTCTAT 241 TCCAGGTATT GTGGACAGCA AAAACCAGAA GATTCTGAGC GATTCCGGCA TCAGCGAAAC 301 GAACATCGAT ATTCAAGATT ATTTCAAAAA GTTTAAGAAC ATCATCAAGC TGAGCATTGA 361 AAATGATGCG AAATCCGCGG CATACGGTGA GTTCTACTTC GGTCAGGACC AAAAACCGAT 421 CAACATGGTG CACCTGACCT TCGGTACCGG CCTGGGTTGC GGTATTATTA TTAACAAAGA 481 GATTTACCGT ACGATTAACC ACAAAGCGGG TGAGATCGGT AAAAGCTACA GCAATATCAA 541 CAGCACGGAA AGCGTTGTAG TTATGGATAC CTCCGTGATC GCGATCCTGA TTAAATACAA 601 GATGGCGACC GGTAAAGAAA TTAGCGGTAA AGAGTTCTTC GAGTTATATG AAAAGAAAGA 661 CCCGTTAGTT CAAAAGCTCA TGAAAGATTG GACCTCAGCT GTTGCGCGCA CGATCATTGG 721 CCTGGATTAC TCATTCGATT TTGATCTGTT GACCATTGGT GGCGGTGTCA GCGACAATAA 781 AATTTTCCAG AAACTGCTGT TTGAGGAACT GGATCTGAAC AAGGACTTCG AGTTTATGGG 841 TAATAAGCTG TCGAGCAACG GTCAAACGAT TAGCAAAGTG AAAATTAGCA ATCTGAAAAA 901 CGATGCCGGT TGTTACGGCA TGTTAGCAAT CTTAAAACAA AACTGAAGAG CTTAGAGACC 961 CCAGCCTCAG ATTGTTGTCT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA 1021 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT 1081 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA 1141 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 1201 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 1261 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 1321 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 1381 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 1441 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 1501 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 1561 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 1621 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 1681 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 1741 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 1801 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 1861 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 1921 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT 1981 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 2041 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA 2101 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC 2161 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT 2221 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT 2281 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 2341 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA 2401 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA 2461 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG 2521 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT 2581 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 2641 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT 2701 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA 2761 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC 2821 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 2881 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC 2941 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG //