LOCUS       Exported     3421 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3421
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1459..1506
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3354..3370
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3371..3402
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1387
                     /label="trmE"
                     /note="Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34."
                     /translation="MNNLNQTIVAPTTNISTQAISLIRISGDDSFNIINKLLKTKIKEEKNVWVRKMFDGQELVDEVVITSFVSPASFTGENVVEIACHGGILNTQRIINLIIKNGARMANKGEFSQRAFLNNKIDLIQAEGINDLIFAKNELALKIGVNNMSGAHNQSIINLKGNLLDIISRIQVSIDYPDYDDVEGSSIPELAKALSGINEEVNNLLKRSKMAIKNTNGIKTAIVGKTNVGKSSLLNALLNEDKAIVTDIHGTTRDIVTGEINLENISLNLIDTAGIRETTDIVEGLGIEKSLKIIDEAELVLFVVDFKSINDEENKMIFDKLENKNYILVFNKSEFINDIERNKIKDLHNNFVYTSAINNDIDNLIMKIEQMHINEEIINNDSLILINLQQITLVEQVKDRLEKSLNAIIGGMPIDIVNVDLHEAWDYLNQLIGEQYDEEIIDNIFKKYCLGK"
     primer_bind     complement(1511..1527)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1594..2182)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2373..3233)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3234..3338)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GAACAATCTG AACCAAACCA TTGTCGCACC
       61 TACCACGAAC ATTAGCACCC AGGCGATCAG CCTGATTCGT ATCAGCGGTG ATGATAGCTT
      121 TAACATCATT AACAAGCTGC TGAAAACTAA AATTAAAGAG GAGAAGAACG TTTGGGTTCG
      181 TAAAATGTTC GACGGTCAGG AGCTGGTGGA CGAAGTTGTG ATTACGAGCT TTGTCTCCCC
      241 GGCGAGCTTT ACCGGTGAGA ACGTCGTCGA GATTGCTTGC CATGGTGGTA TCCTGAATAC
      301 GCAACGTATC ATTAATTTGA TTATCAAGAA CGGCGCGCGT ATGGCTAATA AAGGCGAATT
      361 TAGCCAGCGT GCTTTCCTGA ACAATAAAAT TGATCTGATT CAGGCCGAGG GTATTAACGA
      421 CCTGATTTTC GCAAAGAACG AGTTAGCGCT GAAGATTGGT GTGAATAATA TGAGCGGTGC
      481 CCACAATCAG TCAATTATTA ACCTGAAAGG TAATCTGCTG GACATTATTA GCCGTATCCA
      541 GGTGAGCATC GACTACCCGG ATTACGACGA CGTGGAGGGT TCTTCCATTC CGGAACTGGC
      601 CAAAGCCTTG TCAGGTATTA ATGAAGAGGT CAACAATCTC CTTAAGCGTA GCAAAATGGC
      661 GATCAAAAAC ACCAATGGCA TCAAGACGGC GATCGTCGGT AAAACCAATG TAGGTAAGTC
      721 TAGCCTGCTG AATGCACTGC TGAACGAAGA TAAAGCAATT GTGACTGACA TTCACGGTAC
      781 CACGCGCGAT ATCGTCACTG GTGAAATCAA CCTGGAAAAC ATTAGCCTGA ATCTGATCGA
      841 CACGGCGGGC ATCCGCGAAA CCACTGATAT CGTTGAAGGT CTGGGCATTG AAAAGTCGCT
      901 GAAGATCATC GACGAAGCTG AGCTTGTTCT GTTCGTTGTC GACTTTAAGA GCATTAATGA
      961 TGAGGAAAAC AAGATGATTT TCGATAAGCT GGAAAACAAA AACTACATTC TGGTGTTTAA
     1021 CAAAAGCGAA TTTATTAATG ATATTGAACG CAACAAGATC AAAGATCTGC ATAACAACTT
     1081 TGTGTATACC AGCGCGATTA ATAATGATAT CGACAATCTG ATTATGAAGA TTGAGCAGAT
     1141 GCACATCAAC GAAGAAATTA TTAACAATGA TAGCCTGATT CTGATCAATC TGCAACAGAT
     1201 TACCCTGGTT GAACAGGTTA AAGATCGCCT GGAGAAAAGC CTGAACGCGA TTATTGGCGG
     1261 CATGCCGATC GATATCGTGA ACGTTGATCT GCACGAGGCA TGGGATTATC TGAACCAACT
     1321 GATCGGCGAA CAATATGATG AAGAGATCAT CGATAATATC TTCAAAAAAT ACTGCCTGGG
     1381 TAAGTGAAGA GCTTAGAGAC CGCTTGACGG AGCATCTATC TCGCTGAGGT GTCAATCGTC
     1441 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1501 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1561 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1621 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1681 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1741 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1801 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1861 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1921 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1981 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2041 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2101 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2161 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2221 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2281 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2341 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2401 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2461 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     2521 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     2581 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     2641 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2701 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2761 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2821 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2881 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     2941 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3001 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3061 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3121 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3181 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3241 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3301 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3361 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     3421 G
//