LOCUS       Exported     3676 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3676
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1714..1761
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3609..3625
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3626..3657
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1642
                     /label="Mfl016"
                     /note=""
                     /translation="MNTKKTKTKTFEFLTLFVMVIGTVIGSGIYMKNSELLNQTNNPIIALILWSFVGVICIMSMIVFIEISSSTKHFGNGTLGNWAKIFINRKTASFFSIMYGWVYIPSTQSVFVAGAVNYLLLAIGAPITPYQQLLIYLVVGISIFITCTILSIYKRFINRKIQVVGILIKFLPLLIAFIAGFILIDKTGGTSAWWNGGIDKNTWGTNEWSPILFLGGFGGILFAFDGYVYIANAQKTATNKDVVPKALFAGMIFVAIFYVLMALSLFMGSPDGSIVKLLEKMLGGKESAAARIISNLILMSICLLGSNIFVDIAIVDLASDANNKTIYTKKRSMNYKEAGFIQLIIFIVAYTFLITIGISTTRDGWDGIGSAVGSGSIENVEELLNRPTDYINWFSSGTSALVFIMVLAVMVAGVVNRFTKKVKVEQTKLFMVCGIISSLFMAIFIFFGIFAFIWEPKNIAKGDWGITQSGMWFLIILVVSLTINVIVFLIQEYKFKKDPYLDGWDGEINPNIEIEDSISIKTDLIYLKNKVFKRKEN"
     primer_bind     complement(1766..1782)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1849..2437)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2628..3488)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3489..3593)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GAATACCAAA AAGACTAAAA CCAAGACCTT
       61 CGAATTCCTG ACGCTGTTCG TCATGGTCAT TGGTACTGTT ATCGGCAGCG GCATTTATAT
      121 GAAGAACAGC GAACTGCTGA ACCAAACCAA TAACCCGATC ATTGCCTTGA TCTTGTGGAG
      181 CTTCGTGGGC GTGATCTGCA TTATGTCGAT GATTGTATTT ATCGAAATTT CAAGCAGCAC
      241 GAAGCACTTC GGTAACGGCA CCTTGGGTAA CTGGGCGAAG ATCTTCATCA ACCGCAAGAC
      301 TGCGAGCTTC TTTAGCATCA TGTACGGTTG GGTTTATATT CCGAGCACGC AGTCGGTGTT
      361 CGTCGCGGGT GCAGTTAACT ACCTTTTGCT GGCTATCGGT GCGCCGATCA CTCCGTACCA
      421 GCAGCTGCTG ATCTATCTGG TCGTGGGTAT CTCCATTTTT ATCACGTGCA CCATCCTGAG
      481 CATCTACAAA CGCTTCATTA ACCGTAAAAT CCAGGTGGTG GGTATCCTGA TTAAGTTTCT
      541 GCCGCTGCTG ATTGCCTTTA TTGCTGGCTT TATCCTGATT GATAAAACCG GTGGCACGAG
      601 CGCTTGGTGG AACGGTGGCA TCGACAAAAA CACCTGGGGT ACCAATGAAT GGAGCCCTAT
      661 TCTGTTTTTG GGCGGTTTTG GTGGTATTTT GTTTGCTTTT GATGGTTACG TGTATATCGC
      721 CAATGCTCAA AAAACCGCAA CGAATAAGGA CGTCGTGCCG AAGGCACTGT TTGCGGGTAT
      781 GATCTTCGTC GCAATTTTTT ACGTGCTGAT GGCGTTGAGC CTGTTTATGG GCAGCCCGGA
      841 CGGTAGCATT GTGAAATTGC TGGAGAAGAT GCTGGGCGGT AAGGAGAGCG CGGCAGCTCG
      901 TATCATCAGC AATCTGATCC TGATGAGCAT TTGTCTGCTG GGTAGCAATA TCTTCGTCGA
      961 CATTGCCATT GTGGACTTGG CCTCTGATGC AAACAACAAA ACCATCTATA CGAAGAAGAG
     1021 AAGCATGAAT TACAAGGAAG CTGGTTTCAT CCAACTGATT ATCTTCATCG TTGCCTACAC
     1081 CTTTTTAATC ACCATCGGTA TCTCCACCAC GCGTGATGGT TGGGACGGTA TCGGTTCTGC
     1141 GGTTGGTAGC GGTAGCATTG AGAACGTTGA GGAGCTGCTG AATCGTCCGA CGGACTATAT
     1201 CAACTGGTTT AGCAGCGGCA CGTCTGCACT GGTGTTCATC ATGGTTCTGG CGGTGATGGT
     1261 GGCGGGCGTG GTCAATCGTT TCACGAAAAA AGTCAAGGTC GAACAAACGA AACTGTTCAT
     1321 GGTGTGCGGC ATCATCTCCT CCCTGTTCAT GGCGATTTTC ATCTTTTTCG GCATTTTCGC
     1381 ATTCATCTGG GAGCCAAAGA ACATTGCAAA AGGTGACTGG GGTATCACCC AAAGCGGTAT
     1441 GTGGTTTCTG ATCATCCTGG TCGTTTCTCT CACGATTAAT GTGATTGTGT TCTTAATTCA
     1501 GGAGTACAAG TTTAAAAAGG ACCCATATCT GGACGGCTGG GATGGTGAGA TTAATCCGAA
     1561 TATTGAGATC GAGGACTCTA TTAGCATTAA AACGGACCTG ATTTACCTGA AGAACAAAGT
     1621 TTTCAAGCGT AAGGAGAACT GAAGAGCTTA GAGACCGCGG GTTAGATACT GCAACTCGCT
     1681 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1741 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1801 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1861 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1921 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1981 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     2041 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     2101 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     2161 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     2221 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2281 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2341 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2401 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2461 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2521 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2581 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2641 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2701 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2761 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2821 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2881 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2941 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     3001 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     3061 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     3121 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     3181 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3241 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3301 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3361 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3421 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3481 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3541 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3601 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3661 TCAGGTGGAG TGGGAG
//