LOCUS       Exported     3331 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3331
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1369..1416
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3264..3280
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3281..3312
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1297
                     /label="Mfl017"
                     /note="Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)."
                     /translation="MLDINFIESNLVKVKEQLNKRSGDYSLIIDEAVELNVQRKSILKEVENLKANKNNLSKQVGELMRNKQSDEANKIKEEVTLINSKIEKLDDSLKLVQEQLTSKLQNIPNIPNDNMPIGNDDNDNVEVRQWGNELIKKHNTPHWDIADKLKLVDFEAGPKLSGSRFVVYTGLGAKLVRSLSNILLNLHTSKGYTEITVPLLVNPQAMYGTGQLPKFKEDAYITTNDQYLIPTGEVPLTNLHAGEILDLNQLPIHYTTYSQCFRQEAGSAGRDTKGLIRLHQFNKVELVKITNQESSEAELQAMVNDAEAVLQLFNLPYRVVELCTGDVGFSSSKTYDLEVWFPEQNKYREISSCSNCTDFQARNMQTRYRDANGEVKLAHTLNGSGVAIDRLIAAILENYWDGEKLILPTALKPYFDNKEYID"
     primer_bind     complement(1421..1437)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1504..2092)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2283..3143)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3144..3248)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAGAACGAA CAGGCACTAC GGTCTCAAAT GCTGGATATT AATTTCATTG AGAGCAATCT
       61 GGTGAAGGTG AAGGAGCAAC TGAACAAGCG TTCCGGTGAC TATAGCCTTA TCATCGATGA
      121 AGCAGTGGAG CTGAACGTTC AACGCAAATC GATCTTGAAG GAAGTCGAGA ACCTGAAAGC
      181 TAACAAAAAT AACCTGAGCA AGCAAGTGGG TGAGCTGATG CGTAATAAAC AAAGCGACGA
      241 AGCGAACAAA ATCAAAGAGG AGGTCACGCT GATCAATTCC AAAATCGAGA AACTGGACGA
      301 TTCGCTGAAA CTGGTCCAGG AACAGCTGAC CAGCAAGCTG CAGAACATCC CGAATATTCC
      361 GAACGATAAC ATGCCAATCG GTAACGATGA CAATGATAAT GTGGAGGTGC GTCAATGGGG
      421 TAACGAGCTG ATTAAGAAGC ATAATACCCC GCACTGGGAT ATTGCGGATA AACTGAAGCT
      481 GGTCGATTTC GAAGCGGGCC CGAAGCTGAG CGGCAGCCGC TTCGTGGTGT ATACCGGTCT
      541 GGGCGCTAAG TTGGTCCGTA GCCTGTCAAA TATCCTGTTG AACTTGCATA CGTCCAAAGG
      601 TTATACCGAG ATCACCGTGC CGCTCCTGGT TAACCCGCAA GCCATGTACG GCACGGGTCA
      661 ATTGCCGAAG TTTAAAGAGG ATGCCTATAT TACCACGAAT GACCAGTATC TGATCCCTAC
      721 CGGTGAGGTT CCGCTGACCA ATCTGCACGC TGGTGAGATC CTGGATCTGA ATCAACTGCC
      781 GATTCACTAT ACCACGTACA GCCAATGTTT CCGTCAAGAA GCTGGTTCCG CGGGTCGTGA
      841 CACGAAAGGT CTTATCCGCC TGCACCAGTT CAATAAAGTG GAGCTGGTGA AAATTACCAA
      901 CCAAGAGTCT AGCGAAGCGG AGCTGCAGGC AATGGTGAAT GACGCCGAAG CAGTCCTGCA
      961 GTTGTTCAAT CTGCCATACC GCGTTGTCGA GTTGTGCACT GGTGATGTCG GTTTCTCTAG
     1021 CTCCAAAACC TACGACCTGG AAGTCTGGTT TCCGGAGCAA AACAAGTATC GTGAGATTTC
     1081 CAGCTGCAGC AATTGTACCG ATTTTCAGGC GCGTAATATG CAAACTCGCT ACCGTGATGC
     1141 GAATGGTGAG GTTAAACTGG CTCACACCTT GAACGGTAGC GGTGTGGCGA TTGATCGTCT
     1201 GATCGCCGCG ATCTTGGAGA ATTATTGGGA TGGCGAGAAA CTGATTCTGC CGACCGCCCT
     1261 GAAGCCGTAC TTCGATAACA AAGAATACAT CGATTGAAGA GCTTAGAGAC CGGCCTTTTA
     1321 TTGGGTCCAG TCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1381 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1441 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     1501 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1561 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1621 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1681 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1741 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1801 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1861 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1921 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1981 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2041 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     2101 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     2161 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     2221 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     2281 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2341 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     2401 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     2461 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     2521 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2581 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2641 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2701 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2761 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2821 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2881 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     2941 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     3001 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     3061 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     3121 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     3181 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     3241 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     3301 AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA G
//