LOCUS Exported 3877 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3877 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1915..1962 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3810..3826 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3827..3858 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1843 /note="" /translation="MANNKKNTNTNTWSKATNTKAVNAQKANQVKNESAHNKEIVDKYKEAINSTNKNSKQPTKKVVEPKTSTVVANSNTKKPLSKSEEKLINKINEKELIKQKIAEDKKLKKQNKAEAKRIYQEQIKQEKNRILLEKQEKDLKQIQEAEAKAKKMEAERIADAKKLALEEKAAKQAEKAQAKKMEAERIALEKKAAAEKAKAEKQAEAERIAAAKKLALEEKEREKLIEAQKLIEEKKAAENAKKIEDEKIQKELKTKAEFEAKQKETENKKIAKEERTNMGQTQTEKTPKVGKNSKTSEAREIQVFEPTAESEAKREFIEIDPKTNFDKGVKGLKQKNKYQKQLTSKYSKDILSEGTIVTTTNCKPNTLEHIIELKDVRKSYITGDLETPILKGIDVKLDKGDFIVILGPSGSGKTTFLNVISGLDKATEGDVFILGSNLSLLKDSHLTKFRRRNVGFIFQQYNLLTNLTSKENAEVGQNLANKNKQGMSIEEIFETIGMKEQMNKYPHQMSGGQQQRVSIARALAKNPEILFADEPTGALDEEMGRKVLEILVKVNREQKTTVVVVTHNPNIAKIANTVIHIKNGLIDSLEKNSKPADPKTIEWS" /label="Mfl019" primer_bind complement(1967..1983) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2050..2638) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2829..3689) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3690..3794) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GGCGAATAAC AAAAAGAATA CCAATACCAA 61 TACCTGGAGC AAGGCCACCA ACACGAAAGC AGTCAACGCG CAAAAAGCGA ACCAGGTGAA 121 GAATGAGAGC GCCCATAACA AGGAGATCGT TGACAAATAC AAAGAAGCCA TCAACTCAAC 181 GAACAAGAAT AGCAAGCAGC CGACCAAAAA AGTTGTGGAA CCGAAAACCA GCACGGTGGT 241 CGCGAACTCC AACACCAAGA AGCCTTTGAG CAAAAGCGAA GAGAAGTTGA TTAATAAAAT 301 CAACGAAAAG GAGCTGATCA AACAAAAGAT TGCGGAGGAT AAAAAGTTGA AAAAGCAAAA 361 TAAAGCGGAG GCGAAGCGCA TTTACCAGGA ACAGATCAAG CAGGAGAAGA ACCGTATCCT 421 GCTGGAGAAA CAGGAAAAGG ATTTGAAGCA GATCCAAGAA GCGGAAGCGA AGGCCAAAAA 481 GATGGAGGCC GAACGTATCG CGGATGCGAA AAAATTGGCG CTGGAGGAGA AGGCTGCAAA 541 ACAAGCGGAG AAGGCGCAAG CGAAAAAGAT GGAGGCTGAG CGCATTGCGT TGGAGAAGAA 601 GGCGGCGGCA GAGAAGGCTA AAGCTGAGAA GCAAGCGGAG GCGGAGCGTA TTGCGGCCGC 661 TAAGAAATTG GCTCTGGAGG AGAAAGAACG TGAGAAACTG ATTGAAGCGC AAAAACTGAT 721 TGAAGAAAAG AAGGCGGCCG AAAACGCGAA GAAAATCGAG GATGAGAAGA TTCAAAAAGA 781 ACTCAAGACC AAAGCGGAGT TCGAGGCGAA GCAGAAAGAA ACCGAGAATA AGAAAATTGC 841 GAAAGAGGAA CGCACCAATA TGGGTCAAAC GCAAACTGAG AAAACGCCTA AAGTGGGTAA 901 AAACAGCAAA ACCTCCGAGG CGCGTGAGAT TCAGGTCTTT GAACCGACCG CAGAGAGCGA 961 AGCTAAACGT GAATTTATCG AGATTGACCC GAAAACGAAC TTTGATAAAG GCGTGAAGGG 1021 CCTGAAGCAG AAGAACAAAT ATCAAAAGCA ACTGACCAGC AAGTACAGCA AGGATATTCT 1081 GAGCGAAGGT ACCATCGTGA CCACTACCAA CTGCAAACCG AATACCCTGG AACACATCAT 1141 TGAACTGAAA GACGTGCGTA AGTCTTATAT CACCGGCGAC CTGGAAACCC CGATTCTGAA 1201 AGGTATCGAC GTTAAACTGG ACAAAGGTGA CTTTATTGTG ATCCTGGGCC CGTCTGGCTC 1261 GGGCAAAACC ACCTTCCTGA ACGTGATTAG CGGCCTGGAT AAAGCCACCG AGGGTGACGT 1321 CTTTATTCTG GGTAGCAATC TGAGCTTGCT GAAGGATAGC CACTTAACTA AATTTCGCCG 1381 CCGTAATGTT GGTTTTATTT TCCAACAGTA CAATCTGCTG ACCAATCTGA CCAGCAAAGA 1441 GAACGCAGAA GTTGGTCAGA ATCTGGCCAA CAAAAATAAG CAGGGTATGT CTATCGAGGA 1501 AATTTTTGAA ACCATTGGCA TGAAAGAACA AATGAATAAG TATCCGCATC AGATGAGCGG 1561 TGGCCAACAG CAGCGCGTTA GCATTGCGCG TGCTCTGGCC AAGAACCCGG AGATTTTGTT 1621 TGCAGATGAG CCGACGGGTG CCCTGGATGA GGAAATGGGT CGTAAAGTGT TGGAGATCCT 1681 GGTGAAGGTG AATCGTGAGC AAAAGACCAC CGTCGTCGTG GTGACGCATA ATCCTAACAT 1741 TGCGAAAATC GCCAACACCG TGATCCACAT TAAGAACGGT CTGATCGACA GCTTGGAGAA 1801 AAATTCTAAA CCGGCCGATC CGAAAACGAT TGAGTGGTCC TGAAGAGCTT AGAGACCCGC 1861 ATGTAGGCAC CATCTATCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA 1921 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC 1981 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA 2041 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 2101 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 2161 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 2221 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 2281 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 2341 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 2401 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 2461 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 2521 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 2581 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 2641 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 2701 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 2761 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 2821 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 2881 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 2941 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA 3001 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 3061 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 3121 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 3181 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 3241 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 3301 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 3361 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 3421 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 3481 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 3541 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 3601 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 3661 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 3721 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 3781 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC 3841 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG //