LOCUS       Exported     3877 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3877
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1915..1962
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3810..3826
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3827..3858
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1843
                     /note=""
                     /translation="MANNKKNTNTNTWSKATNTKAVNAQKANQVKNESAHNKEIVDKYKEAINSTNKNSKQPTKKVVEPKTSTVVANSNTKKPLSKSEEKLINKINEKELIKQKIAEDKKLKKQNKAEAKRIYQEQIKQEKNRILLEKQEKDLKQIQEAEAKAKKMEAERIADAKKLALEEKAAKQAEKAQAKKMEAERIALEKKAAAEKAKAEKQAEAERIAAAKKLALEEKEREKLIEAQKLIEEKKAAENAKKIEDEKIQKELKTKAEFEAKQKETENKKIAKEERTNMGQTQTEKTPKVGKNSKTSEAREIQVFEPTAESEAKREFIEIDPKTNFDKGVKGLKQKNKYQKQLTSKYSKDILSEGTIVTTTNCKPNTLEHIIELKDVRKSYITGDLETPILKGIDVKLDKGDFIVILGPSGSGKTTFLNVISGLDKATEGDVFILGSNLSLLKDSHLTKFRRRNVGFIFQQYNLLTNLTSKENAEVGQNLANKNKQGMSIEEIFETIGMKEQMNKYPHQMSGGQQQRVSIARALAKNPEILFADEPTGALDEEMGRKVLEILVKVNREQKTTVVVVTHNPNIAKIANTVIHIKNGLIDSLEKNSKPADPKTIEWS"
                     /label="Mfl019"
     primer_bind     complement(1967..1983)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2050..2638)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2829..3689)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3690..3794)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GGCGAATAAC AAAAAGAATA CCAATACCAA
       61 TACCTGGAGC AAGGCCACCA ACACGAAAGC AGTCAACGCG CAAAAAGCGA ACCAGGTGAA
      121 GAATGAGAGC GCCCATAACA AGGAGATCGT TGACAAATAC AAAGAAGCCA TCAACTCAAC
      181 GAACAAGAAT AGCAAGCAGC CGACCAAAAA AGTTGTGGAA CCGAAAACCA GCACGGTGGT
      241 CGCGAACTCC AACACCAAGA AGCCTTTGAG CAAAAGCGAA GAGAAGTTGA TTAATAAAAT
      301 CAACGAAAAG GAGCTGATCA AACAAAAGAT TGCGGAGGAT AAAAAGTTGA AAAAGCAAAA
      361 TAAAGCGGAG GCGAAGCGCA TTTACCAGGA ACAGATCAAG CAGGAGAAGA ACCGTATCCT
      421 GCTGGAGAAA CAGGAAAAGG ATTTGAAGCA GATCCAAGAA GCGGAAGCGA AGGCCAAAAA
      481 GATGGAGGCC GAACGTATCG CGGATGCGAA AAAATTGGCG CTGGAGGAGA AGGCTGCAAA
      541 ACAAGCGGAG AAGGCGCAAG CGAAAAAGAT GGAGGCTGAG CGCATTGCGT TGGAGAAGAA
      601 GGCGGCGGCA GAGAAGGCTA AAGCTGAGAA GCAAGCGGAG GCGGAGCGTA TTGCGGCCGC
      661 TAAGAAATTG GCTCTGGAGG AGAAAGAACG TGAGAAACTG ATTGAAGCGC AAAAACTGAT
      721 TGAAGAAAAG AAGGCGGCCG AAAACGCGAA GAAAATCGAG GATGAGAAGA TTCAAAAAGA
      781 ACTCAAGACC AAAGCGGAGT TCGAGGCGAA GCAGAAAGAA ACCGAGAATA AGAAAATTGC
      841 GAAAGAGGAA CGCACCAATA TGGGTCAAAC GCAAACTGAG AAAACGCCTA AAGTGGGTAA
      901 AAACAGCAAA ACCTCCGAGG CGCGTGAGAT TCAGGTCTTT GAACCGACCG CAGAGAGCGA
      961 AGCTAAACGT GAATTTATCG AGATTGACCC GAAAACGAAC TTTGATAAAG GCGTGAAGGG
     1021 CCTGAAGCAG AAGAACAAAT ATCAAAAGCA ACTGACCAGC AAGTACAGCA AGGATATTCT
     1081 GAGCGAAGGT ACCATCGTGA CCACTACCAA CTGCAAACCG AATACCCTGG AACACATCAT
     1141 TGAACTGAAA GACGTGCGTA AGTCTTATAT CACCGGCGAC CTGGAAACCC CGATTCTGAA
     1201 AGGTATCGAC GTTAAACTGG ACAAAGGTGA CTTTATTGTG ATCCTGGGCC CGTCTGGCTC
     1261 GGGCAAAACC ACCTTCCTGA ACGTGATTAG CGGCCTGGAT AAAGCCACCG AGGGTGACGT
     1321 CTTTATTCTG GGTAGCAATC TGAGCTTGCT GAAGGATAGC CACTTAACTA AATTTCGCCG
     1381 CCGTAATGTT GGTTTTATTT TCCAACAGTA CAATCTGCTG ACCAATCTGA CCAGCAAAGA
     1441 GAACGCAGAA GTTGGTCAGA ATCTGGCCAA CAAAAATAAG CAGGGTATGT CTATCGAGGA
     1501 AATTTTTGAA ACCATTGGCA TGAAAGAACA AATGAATAAG TATCCGCATC AGATGAGCGG
     1561 TGGCCAACAG CAGCGCGTTA GCATTGCGCG TGCTCTGGCC AAGAACCCGG AGATTTTGTT
     1621 TGCAGATGAG CCGACGGGTG CCCTGGATGA GGAAATGGGT CGTAAAGTGT TGGAGATCCT
     1681 GGTGAAGGTG AATCGTGAGC AAAAGACCAC CGTCGTCGTG GTGACGCATA ATCCTAACAT
     1741 TGCGAAAATC GCCAACACCG TGATCCACAT TAAGAACGGT CTGATCGACA GCTTGGAGAA
     1801 AAATTCTAAA CCGGCCGATC CGAAAACGAT TGAGTGGTCC TGAAGAGCTT AGAGACCCGC
     1861 ATGTAGGCAC CATCTATCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1921 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1981 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     2041 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     2101 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     2161 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     2221 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     2281 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     2341 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     2401 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     2461 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     2521 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2581 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2641 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     2701 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2761 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2821 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2881 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2941 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     3001 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     3061 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     3121 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     3181 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     3241 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     3301 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     3361 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     3421 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     3481 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     3541 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     3601 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     3661 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     3721 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3781 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3841 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//