LOCUS       Exported     4142 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4142
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4108
                     /translation="MKKLLLTLSSVTLVGTAGMSVISCGVKPEKSVIFMLPGEAVGVGSTDKIDAYNDLVDEFNELHKNDSGFAPIKVKWSKSGTINDSILTGDNLPDLYISYADAVSLYANTKVSNQVRDMEASLGEAGFANLKNDIIDDAFLQEGQYKEQGSDKATQVVLPFGKSVEMSVINVNFFLEFISKISITDFDGASISPKVKTEFEKFNVNERKNLTNETGAMSKTTIFSDKTPKLNEQWFTEHGLNDALTTLQTALAPLTSVGSSSYTGESVDDLIRTIFAKNDNIIALAQVYNEIFNVESNIEVKYASKDGSVVVDPSNGKHFSFGIDSLANKYFMDHAARTNSGLIDITNEDNGFFYSANYDKATRVANVNFNKNSVGFNDTSEFLQAFKDIAVKNNKSGGSYAEQWNNTLNLSRQEGTTKYYTSDSFLNGSSFMSSGSSAGAYNFTKTKYVNNTGYNPVTNADVITTSTSTKEGKNSVFMSQGPGIAGFKSNGSNAAEKEKTVTEFLNYIMQPKQAADFALKSNYMPATKSGMSIYQNYVNGNYNNTKANNQSNAIVNPEALQNVVAELNTKEGTTKYTVDNTFTAIYSTSKGSLSSQASPNAVNSGFIEKYLVGNSERILVASTPTPIGTTVRDSIATAITGSGTITDLSKAPGIKFVEILDASNTVYTLQNYVMKKNNTDMFSKINLTHNAK"
                     /label="Mfl023"
                     /note=""
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCAAA TGAAAAAGTT
     2041 GCTGCTGACC CTGTCCAGCG TCACGCTGGT TGGTACTGCA GGCATGAGCG TTATCAGCTG
     2101 CGGTGTTAAA CCAGAGAAGT CTGTAATCTT CATGTTGCCG GGCGAAGCAG TTGGTGTTGG
     2161 CAGCACCGAC AAAATCGACG CGTACAACGA CCTGGTCGAC GAATTCAATG AGTTGCACAA
     2221 GAATGATAGC GGTTTTGCCC CGATCAAGGT CAAGTGGTCC AAGAGCGGCA CCATTAATGA
     2281 TAGCATCCTG ACCGGTGACA ACTTGCCGGA CCTGTACATC AGCTACGCCG ACGCAGTTAG
     2341 CTTGTATGCA AATACCAAGG TTAGCAACCA GGTTCGTGAT ATGGAAGCGA GCCTGGGCGA
     2401 GGCGGGTTTT GCGAACCTGA AGAATGACAT TATTGACGAT GCATTCCTCC AAGAGGGCCA
     2461 GTATAAAGAG CAAGGTAGCG ACAAAGCAAC CCAAGTCGTG CTGCCGTTCG GTAAGAGCGT
     2521 TGAGATGTCC GTTATTAACG TGAATTTCTT CCTGGAGTTT ATTAGCAAAA TCAGCATTAC
     2581 CGACTTCGAT GGTGCCTCGA TTAGCCCGAA GGTCAAGACT GAGTTTGAAA AGTTCAATGT
     2641 GAACGAACGT AAAAACCTGA CCAACGAAAC CGGTGCCATG TCTAAAACCA CCATCTTTTC
     2701 TGACAAAACC CCGAAATTGA ATGAACAATG GTTCACCGAG CACGGCCTGA ACGACGCCTT
     2761 AACGACCCTG CAAACCGCGC TGGCACCGTT AACCTCAGTA GGTTCTTCGT CTTATACGGG
     2821 TGAGTCTGTA GACGATTTGA TCCGCACTAT TTTTGCAAAA AATGATAACA TCATTGCCCT
     2881 GGCTCAGGTC TATAACGAAA TCTTCAACGT GGAGAGCAAC ATCGAAGTAA AGTACGCCAG
     2941 CAAAGATGGT TCCGTAGTCG TCGACCCGAG CAATGGTAAG CACTTCAGCT TTGGCATCGA
     3001 CTCCCTGGCG AACAAGTATT TTATGGACCA TGCGGCTCGC ACCAATAGCG GCCTGATTGA
     3061 TATCACGAAT GAGGATAATG GCTTCTTTTA CAGCGCCAAC TATGACAAAG CAACGCGCGT
     3121 TGCAAATGTT AATTTCAATA AGAATAGCGT TGGCTTCAAT GACACGAGCG AGTTTCTGCA
     3181 GGCTTTTAAG GACATTGCAG TGAAGAACAA TAAATCTGGC GGCAGCTATG CAGAGCAGTG
     3241 GAATAATACC CTGAACCTGA GCAGACAGGA GGGCACCACG AAGTACTACA CCTCAGACAG
     3301 CTTCCTGAAC GGCAGCAGCT TCATGAGCAG CGGTTCCTCC GCGGGCGCAT ACAACTTCAC
     3361 CAAGACTAAA TACGTCAATA ATACCGGCTA CAACCCTGTG ACCAATGCCG ACGTTATCAC
     3421 GACGAGCACC TCCACCAAAG AAGGTAAGAA TAGCGTCTTT ATGTCTCAGG GTCCGGGTAT
     3481 TGCCGGTTTC AAGAGCAATG GCAGCAACGC GGCCGAAAAA GAAAAGACCG TCACGGAATT
     3541 CCTGAACTAT ATTATGCAGC CAAAACAGGC TGCAGACTTC GCGCTGAAAA GCAATTACAT
     3601 GCCGGCGACT AAGAGCGGTA TGAGCATTTA TCAGAACTAT GTGAATGGTA ATTACAACAA
     3661 TACGAAAGCA AATAACCAAA GCAACGCGAT CGTGAACCCG GAGGCGCTGC AGAACGTGGT
     3721 AGCAGAGCTG AACACGAAAG AAGGTACGAC CAAGTACACC GTTGATAACA CGTTTACGGC
     3781 GATCTATAGC ACGAGCAAAG GCTCCCTGTC CAGCCAAGCG AGCCCGAATG CCGTAAATAG
     3841 CGGTTTCATT GAGAAGTATC TGGTCGGCAA CAGCGAGCGT ATTCTGGTTG CCAGCACCCC
     3901 GACCCCGATT GGTACCACGG TCCGCGATAG CATTGCCACC GCCATTACCG GCAGCGGCAC
     3961 GATCACTGAT CTGAGCAAGG CACCGGGCAT TAAGTTTGTG GAAATTCTGG ATGCTTCCAA
     4021 CACGGTTTAT ACGCTGCAGA ATTACGTTAT GAAGAAAAAC AATACTGACA TGTTCAGCAA
     4081 AATTAACCTG ACCCACAACG CCAAGTGAAG AGCTTAGAGA CCGCCATCCT CTTATCTCGT
     4141 GG
//