LOCUS       Exported     4421 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4421
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4387
                     /label="Mfl024"
                     /note=""
                     /translation="MSIKLKNITIDYGNFLAVNDLSVNINKGELVSLLGPSGCGKTTALNAIAGLINTTSGQILFDGVDVTHKTPQQRNIGLVFQSYALYSHMSVFKNIAYPLYHSKDFKKELARDNFKYKNRLKDLKQTKSYELIIKQQNDFLNDLKSFLKLTNNNIKLLETEFLKKHKDSIISYTDQLFINYENSEVLKNHLVSYLYDKLKIKFNEIESVIESTILKKYKLLLTHKLDVRFQGTIGYYSWLNEKNVKDNKKHLAELIKTSKISKKQIRKEQKKVSAELDIFTASSTENGYDNYINSKNLITNSFIENEVAELEKEINDYFDKVLLKTKEPIIKNNKIENSISNISKKINAFKVSYLSKHEKLIVKYIKSISNKFPNEIEIKDNLINYLFNISKIEFNSVQEKLNELIFENMQSFVNSCDKDEFTQLINYYPLNVNAYLEVSESSNELLKINNELQKNTDFDTLNDNEHYKNYLTEKEQLTESLLSEFVVKLENFISETFDKLPILVNNELSKNSIKIDISELESEIRKNIFSKKRKIRSLVLEAAKQVEIETQLNKKPSELSGGQQQRVAIARAVVKKPSILLMDEPLSNLDAKLRLTTREWIKKFQQKVGITTVFVTHDQEEAMSISDSIFVMNKGVLQQGGTPLEIYNKPKNKFVANFIGTPNINFIDVKVKKSIFELPSGEEMTIKNLKIEKENWTLGIRPERISISGTKGKIFIGEGEVSLVEQLGKQNHIKVRLNKELEITLILEPHEWNNLKNSSVLKLYARKEDLYLFDTNTNLVEVKYA"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGCCCAG AAGGATACAA CGGTCTCAAA TGAGCATCAA
     2041 GCTGAAGAAC ATCACTATTG ATTATGGTAA TTTCCTGGCG GTGAACGATC TCAGCGTGAA
     2101 TATCAATAAG GGCGAGTTAG TTTCTCTGCT CGGTCCGAGC GGTTGCGGTA AAACGACCGC
     2161 GCTGAATGCG ATTGCCGGCC TGATTAATAC CACCTCCGGT CAAATCCTGT TTGACGGCGT
     2221 TGACGTCACC CATAAAACCC CGCAGCAGCG CAACATTGGC CTGGTTTTTC AGAGCTATGC
     2281 GCTGTATTCT CACATGAGCG TTTTTAAAAA TATCGCTTAT CCGCTGTATC ACAGCAAAGA
     2341 TTTCAAAAAG GAGTTGGCGC GCGATAACTT TAAGTACAAA AACCGTCTGA AAGACCTGAA
     2401 ACAGACGAAG TCCTATGAAC TGATCATTAA GCAGCAAAAT GACTTTCTGA ATGACCTGAA
     2461 AAGCTTCCTG AAACTGACGA ATAATAACAT CAAGCTGCTG GAAACCGAGT TCCTGAAAAA
     2521 ACACAAAGAC TCTATCATTA GCTATACGGA TCAGCTGTTC ATCAATTACG AGAATAGCGA
     2581 GGTCCTGAAA AACCACCTGG TGAGCTATCT GTACGACAAA CTGAAGATTA AATTTAACGA
     2641 GATTGAGAGC GTGATCGAAT CCACCATCCT GAAAAAATAC AAACTGTTGC TGACTCATAA
     2701 GCTGGATGTG CGTTTCCAAG GTACGATTGG TTACTATAGC TGGCTTAACG AGAAAAATGT
     2761 GAAAGACAAC AAGAAACACC TGGCAGAGCT GATTAAGACG TCGAAGATTA GCAAAAAGCA
     2821 GATTCGCAAG GAACAGAAGA AGGTCAGCGC CGAACTGGAT ATTTTTACCG CGTCCAGCAC
     2881 TGAAAACGGT TACGACAACT ACATTAACTC CAAGAATCTG ATTACGAATT CATTCATTGA
     2941 GAACGAAGTC GCTGAGCTGG AGAAAGAAAT TAATGACTAC TTCGATAAAG TTCTGCTGAA
     3001 AACGAAGGAA CCGATCATTA AGAACAACAA AATTGAGAAC AGCATCAGCA ACATTAGCAA
     3061 GAAAATTAAC GCTTTTAAGG TTAGCTATCT GAGCAAACAC GAGAAGTTGA TTGTTAAGTA
     3121 CATTAAGAGC ATTAGCAACA AGTTCCCGAA TGAGATTGAA ATTAAAGATA ACCTGATTAA
     3181 CTATCTGTTT AACATCAGCA AGATTGAATT TAACAGCGTT CAGGAGAAGC TGAATGAACT
     3241 GATCTTTGAA AACATGCAGA GCTTCGTGAA TTCGTGTGAC AAAGATGAAT TCACCCAGCT
     3301 GATCAACTAT TACCCTCTGA ACGTGAACGC GTACCTGGAG GTCAGCGAAA GCAGCAATGA
     3361 GCTGCTGAAG ATCAATAATG AGCTGCAGAA GAACACCGAC TTTGACACCC TGAACGACAA
     3421 CGAGCACTAC AAAAACTACT TGACGGAGAA AGAGCAGCTC ACGGAAAGCC TGCTGAGCGA
     3481 ATTCGTTGTT AAGTTGGAGA ATTTTATCAG CGAAACCTTC GATAAGCTGC CGATCCTGGT
     3541 GAATAACGAG CTGAGCAAAA ACAGCATCAA AATCGACATC TCTGAACTGG AGTCTGAAAT
     3601 CCGTAAAAAC ATTTTCAGCA AAAAACGCAA GATCCGTAGC CTGGTCCTGG AGGCAGCTAA
     3661 ACAGGTCGAG ATCGAAACGC AATTGAACAA GAAACCGTCC GAGCTGAGCG GTGGCCAGCA
     3721 GCAGCGTGTG GCTATTGCCC GTGCAGTGGT GAAAAAACCG TCCATTCTGC TGATGGATGA
     3781 GCCGCTGAGC AATCTGGACG CTAAACTGCG CCTGACGACG CGTGAATGGA TCAAAAAGTT
     3841 CCAACAGAAG GTTGGCATCA CCACGGTTTT CGTGACCCAC GACCAAGAAG AAGCAATGAG
     3901 CATCAGCGAC TCCATCTTCG TCATGAATAA AGGTGTGCTG CAGCAAGGTG GTACGCCGTT
     3961 GGAGATCTAT AACAAGCCAA AGAACAAGTT TGTTGCGAAT TTTATTGGCA CGCCTAATAT
     4021 TAATTTCATC GACGTTAAGG TGAAAAAGAG CATCTTCGAA CTGCCGAGCG GTGAGGAGAT
     4081 GACCATCAAA AACTTAAAAA TCGAGAAGGA AAATTGGACC CTGGGTATCC GCCCGGAGCG
     4141 CATCAGCATT TCCGGCACGA AGGGCAAGAT TTTCATCGGC GAGGGTGAAG TGAGCCTGGT
     4201 GGAACAACTG GGCAAACAAA ACCATATTAA AGTTCGTCTG AACAAGGAGC TGGAGATTAC
     4261 CCTGATCCTG GAGCCGCACG AGTGGAATAA CTTGAAAAAC TCCTCTGTCC TGAAACTGTA
     4321 CGCCCGTAAG GAGGACCTCT ATCTGTTCGA TACCAACACC AACCTCGTGG AAGTTAAATA
     4381 CGCCTGAAGA GCTTAGAGAC CCCATCTTGC GTCGTGAGAA T
//