LOCUS Exported 3515 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3515 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3481 /translation="MKFEKKDFLWGGATSASQVEGAFDAEGKSLTIAEMRPFNPNLDRKSVDELNKYTRSDYEKSIDNKDNLHYPKRIGIDFYHRYKEDIALFKEAGMNIYRMSIAWSRIFPNGDEAQPNAAGIKFYKDVFEECKKNGMEVMLTIQHYDVPYPITKKYGGWSNKKVIDLYIKFATVIMKEYKDLVKYWLPFNEINVATLAPTTGLGIFREDFDTTEDYKNASWQGLHNQFYAQAKVIELAKSISSNIKIGCMVANMTTYSSDCNPVNIWENLATQQMQRYFYYDVMVKGDYPSYSKRYFKENNIKFETTPEEMEVIKNNNIQYITFSYYMTSTISKELKDAAGGNLMMGGKNPFLKATEWGWQIDPIGLRITLNELWDRYQLPLFISENGIGVIETLNENNTVDDDYRIEYLSKHFEQINEAIKDGVDVFGYTMWTPIDVVSLSTNEMSKRYGLIFVDYDDYHKGTGNRFKKKSFEWFKNFIKTKEL" /label="Mfl034" /note="" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAGGAACTA TGGCATCGAG CGGTCTCAAA TGAAATTTGA 2041 GAAAAAAGAC TTCCTGTGGG GCGGCGCGAC CAGCGCCAGC CAAGTTGAAG GCGCGTTCGA 2101 CGCAGAAGGC AAAAGCCTGA CGATCGCGGA GATGCGTCCG TTCAACCCTA ACCTGGATCG 2161 TAAAAGCGTT GACGAGCTGA ACAAGTACAC CCGTAGCGAC TATGAAAAGA GCATTGATAA 2221 TAAGGACAAT CTGCACTATC CGAAGCGTAT CGGCATTGAT TTCTATCATC GTTACAAGGA 2281 GGATATCGCC CTGTTTAAAG AAGCGGGCAT GAATATCTAT CGCATGAGCA TTGCATGGAG 2341 CCGCATCTTC CCGAATGGTG ACGAGGCGCA ACCAAATGCA GCCGGTATTA AGTTTTACAA 2401 AGATGTATTC GAGGAGTGCA AGAAAAACGG CATGGAGGTT ATGCTGACCA TCCAACATTA 2461 CGACGTTCCG TATCCGATTA CCAAGAAATA CGGCGGCTGG TCCAACAAAA AGGTCATTGA 2521 TCTGTACATT AAGTTTGCGA CCGTCATTAT GAAGGAGTAC AAAGATCTGG TGAAGTACTG 2581 GCTGCCGTTT AACGAAATCA ACGTTGCGAC GCTCGCACCG ACCACCGGTC TGGGTATCTT 2641 TCGTGAAGAT TTTGATACCA CCGAGGATTA CAAAAATGCT AGCTGGCAAG GCTTGCACAA 2701 TCAGTTTTAT GCGCAAGCTA AGGTGATCGA ACTGGCTAAA TCAATTAGCT CCAATATCAA 2761 AATTGGTTGC ATGGTGGCGA ATATGACGAC GTACAGCAGC GACTGTAACC CGGTGAACAT 2821 TTGGGAGAAC CTGGCGACGC AACAGATGCA ACGTTACTTC TATTACGATG TGATGGTGAA 2881 GGGTGACTAC CCGAGCTACA GCAAGCGCTA CTTCAAAGAA AATAACATTA AATTCGAAAC 2941 CACGCCGGAA GAAATGGAAG TGATTAAGAA TAATAACATT CAGTATATCA CGTTCTCTTA 3001 CTACATGACG TCTACCATCA GCAAGGAGTT GAAAGATGCT GCGGGCGGTA ACCTGATGAT 3061 GGGCGGTAAA AATCCTTTCC TGAAAGCGAC CGAGTGGGGC TGGCAAATTG ATCCGATCGG 3121 CCTGCGCATT ACCCTTAACG AGCTGTGGGA CCGTTATCAG CTGCCGTTAT TCATTAGCGA 3181 GAACGGTATC GGTGTTATTG AAACCCTGAA CGAAAACAAC ACGGTTGATG ACGATTACCG 3241 CATTGAGTAC CTGAGCAAAC ACTTCGAGCA AATTAATGAG GCCATTAAGG ATGGCGTAGA 3301 TGTGTTCGGC TATACGATGT GGACGCCGAT TGACGTGGTT AGCCTGAGCA CGAACGAGAT 3361 GAGCAAGCGT TATGGTTTGA TCTTTGTTGA TTATGATGAC TATCACAAGG GTACCGGCAA 3421 TCGTTTCAAA AAGAAAAGCT TCGAATGGTT TAAAAATTTC ATTAAAACGA AAGAGCTGTG 3481 AAGAGCTTAG AGACCCCGGT TGTACCTATC GAGTG //