LOCUS       Exported     3593 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3593
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3559
                     /label="Mfl036"
                     /note="Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation."
                     /translation="MPKKFYVTTPIYYPSGNLHIGHAYTTTLADILKRYKDMQGYETFFLTGSDEHGQKIEEKATEAGLTPIDYLEDKVESFKTLWKALKIDYTKFIRTTDSYHEETVKKIFTLLLDKGFIYKGNYEGLYCISCEEFLNADQIDENNMCKISNTKLELVKEETYFLKTSLFQEFMENEVLASEFLIPAYRRNEMLNSFVKPELKDLSVTRTSFSWGIPINEDPKHVVYVWLDALTNYITALGYLQNDDSNFQKFWADENTEILQLAGKEIIRFHSIYWPIILKSLGLRQPTHLLGHGWILSKDTKMSKSLGNVIDPIEMINKYGADALRFYIGYELPTEKDGNFTDDLFIESFNAHLANNVGNLISRINQMITKYFDGFIENDNIVYDDNLTAKGIETINLYIQNMDQYKISDAIRNILDLGNACNKYIEETMPWNLAKEEKIDELKNVMATLQRNISIMVFLLKPILVENYEDMIEQIGLSGVELTFDKINNWDKIIFKKLGDKKIIFKRVN"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGAATTAC TGACCCCTCG GGGTCTCAAA TGCCTAAAAA
     2041 ATTTTACGTC ACGACCCCGA TTTATTACCC TTCCGGCAAT CTGCACATCG GCCACGCATA
     2101 CACCACCACC TTGGCAGACA TTCTGAAACG TTATAAGGAC ATGCAAGGTT ATGAAACGTT
     2161 CTTCCTTACC GGTAGCGACG AACATGGTCA GAAAATTGAG GAGAAGGCCA CCGAGGCTGG
     2221 TCTGACCCCG ATTGACTACC TGGAGGATAA AGTGGAGAGC TTTAAGACTC TGTGGAAGGC
     2281 GTTAAAAATC GATTATACCA AGTTTATTCG TACCACTGAC AGCTACCACG AGGAAACCGT
     2341 GAAGAAAATT TTTACCCTGC TGCTGGATAA AGGTTTCATT TATAAGGGTA ACTATGAAGG
     2401 TCTGTATTGC ATTAGCTGCG AAGAATTCTT GAACGCCGAT CAGATCGACG AGAATAACAT
     2461 GTGTAAAATT TCCAATACGA AGTTGGAGCT GGTCAAGGAG GAAACGTATT TCCTGAAAAC
     2521 GAGCCTTTTC CAAGAATTTA TGGAGAACGA GGTCTTAGCA AGCGAGTTCC TGATTCCTGC
     2581 ATATCGTCGT AACGAAATGT TGAATAGCTT TGTTAAGCCG GAGCTCAAGG ACCTGAGCGT
     2641 TACCCGCACG AGCTTCTCTT GGGGTATTCC AATTAACGAA GATCCGAAGC ACGTTGTGTA
     2701 TGTCTGGCTG GACGCACTGA CCAATTACAT CACGGCGTTG GGCTACTTGC AGAACGATGA
     2761 CAGCAACTTC CAGAAATTCT GGGCCGATGA AAACACGGAA ATCCTGCAGC TGGCCGGCAA
     2821 GGAAATTATT CGTTTCCACA GCATCTACTG GCCGATTATT CTGAAAAGCC TGGGTTTGCG
     2881 TCAACCGACC CATCTGCTGG GTCACGGTTG GATCCTGAGC AAAGACACGA AAATGTCGAA
     2941 AAGCCTGGGT AACGTTATTG ATCCGATTGA AATGATTAAT AAATATGGTG CAGACGCCCT
     3001 GCGTTTTTAC ATCGGTTACG AATTGCCGAC GGAGAAAGAT GGTAATTTCA CGGATGACCT
     3061 GTTTATTGAG AGCTTTAATG CCCACCTGGC TAACAATGTG GGTAATCTGA TCAGCAGAAT
     3121 TAACCAGATG ATTACGAAGT ACTTCGACGG TTTCATCGAG AATGATAACA TTGTTTACGA
     3181 TGATAATCTG ACCGCGAAAG GCATTGAAAC GATCAACCTG TATATTCAAA ACATGGATCA
     3241 ATACAAGATC TCGGATGCTA TTCGCAACAT TCTGGACCTG GGTAACGCGT GCAATAAATA
     3301 CATTGAAGAA ACCATGCCAT GGAATCTGGC GAAGGAGGAA AAAATTGACG AACTGAAGAA
     3361 TGTCATGGCG ACCCTGCAAC GTAACATCAG CATTATGGTT TTTCTGCTGA AGCCTATTCT
     3421 GGTCGAGAAC TACGAGGACA TGATTGAGCA AATTGGCTTA TCCGGTGTCG AACTGACCTT
     3481 CGACAAGATC AACAATTGGG ACAAAATCAT CTTTAAAAAG CTGGGCGACA AGAAGATTAT
     3541 TTTTAAACGT GTTAACTGAA GAGCTTAGAG ACCGGCTGCC AAAGAAACTG ATT
//