LOCUS       Exported     3421 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3421
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1459..1506
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3354..3370
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3371..3402
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1387
                     /label="Mfl037"
                     /note=""
                     /translation="MKVIVLGTNHAGTTAVRTLKRLNPEIEVTTYDRNDVISFLGCGIALWVKGEVKDPNGLFYATPEILESEGINVKMKHEWVSIDADKKTVLIKNLETGETFEDNYDKVIVATGTWPLLPPIPGLDLKGVQICKNYDHAKKIQQANLDDSIKKVTVVGAGYIGVELVDAFVAHGKEVTLVDFADRIMPVYYDAEFTKHVEERMEKAGVKLALSQGVKEFKGANGKVTHVVTDKEEIPTDYVIFSVGVVAQTKQLEGVVELNDRKAILTNEYCQSSNPDIYAIGDCSTVFNKALNMEMPIQLATTAVRTGILAAANIVNGNKLASPGFTGANGIEVFGFKMASAGVSETSAKKMGLDYEAILLSDSDRPEFMSTYKEAWIKLVWDKKTRKIIGAQVASENNHTEIMYMLSLGIQKELTIDELPLVDIFFLPHFNKPYNFVTLAGLEVLGLNYFKK"
     primer_bind     complement(1511..1527)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1594..2182)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2373..3233)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3234..3338)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GAAAGTGATC GTTTTGGGTA CGAATCACGC
       61 GGGTACCACC GCGGTTCGTA CCCTGAAACG CCTGAACCCG GAGATCGAAG TTACCACCTA
      121 CGACCGCAAC GACGTGATTA GCTTTCTGGG TTGCGGTATC GCCCTGTGGG TTAAGGGTGA
      181 AGTGAAGGAC CCGAACGGTT TGTTCTACGC GACGCCGGAA ATTTTGGAGA GCGAAGGCAT
      241 CAATGTCAAA ATGAAGCACG AATGGGTTAG CATCGACGCG GATAAAAAGA CCGTGCTGAT
      301 TAAAAATTTG GAAACGGGCG AAACCTTTGA GGACAATTAC GACAAGGTTA TTGTGGCGAC
      361 CGGTACCTGG CCGTTACTGC CTCCGATTCC AGGTCTGGAT CTGAAGGGTG TCCAGATCTG
      421 TAAGAATTAT GACCACGCAA AGAAGATCCA ACAAGCGAAC CTGGACGACA GCATTAAGAA
      481 AGTGACGGTT GTGGGCGCTG GCTACATTGG CGTTGAACTG GTGGATGCAT TTGTCGCGCA
      541 TGGTAAAGAG GTGACGCTGG TTGATTTCGC GGACCGTATT ATGCCGGTGT ATTATGACGC
      601 GGAGTTTACC AAACACGTCG AGGAGCGTAT GGAAAAGGCT GGTGTGAAGC TGGCGCTGTC
      661 ACAGGGCGTC AAAGAATTCA AAGGCGCAAA CGGCAAGGTC ACGCATGTGG TCACCGATAA
      721 AGAGGAAATC CCGACGGACT ATGTGATTTT CAGCGTGGGT GTGGTTGCTC AGACCAAACA
      781 GCTGGAGGGT GTTGTTGAGT TGAATGACCG TAAGGCTATT CTGACGAATG AGTACTGCCA
      841 GTCGAGCAAT CCGGACATCT ACGCTATTGG TGACTGCAGC ACCGTTTTCA ACAAAGCGCT
      901 GAACATGGAA ATGCCGATTC AACTGGCGAC CACCGCGGTC CGCACCGGCA TCTTGGCAGC
      961 TGCGAACATT GTTAATGGCA ATAAGCTGGC TAGCCCGGGT TTTACGGGCG CCAACGGTAT
     1021 CGAGGTGTTT GGTTTCAAGA TGGCTAGCGC GGGTGTTAGC GAAACCTCTG CCAAGAAAAT
     1081 GGGCCTGGAC TACGAGGCGA TCCTGTTGTC TGATAGCGAC CGTCCTGAAT TCATGAGCAC
     1141 CTATAAAGAG GCATGGATCA AACTGGTTTG GGATAAAAAG ACCCGTAAGA TTATTGGTGC
     1201 TCAGGTCGCC AGCGAAAATA ACCATACGGA GATCATGTAC ATGCTGAGCC TGGGTATTCA
     1261 AAAAGAACTG ACCATTGATG AACTGCCACT GGTCGACATC TTCTTTCTGC CGCACTTTAA
     1321 TAAACCGTAC AATTTTGTTA CGTTGGCTGG CTTGGAAGTG CTGGGCCTCA ACTACTTTAA
     1381 GAAGTGAAGA GCTTAGAGAC CGTGTCACAT TGTGTCGGAC TCGCTGAGGT GTCAATCGTC
     1441 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1501 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1561 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1621 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1681 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1741 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1801 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1861 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1921 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1981 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2041 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2101 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2161 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2221 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2281 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2341 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2401 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2461 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     2521 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     2581 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     2641 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2701 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2761 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2821 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2881 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     2941 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3001 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3061 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3121 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3181 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3241 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3301 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3361 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     3421 G
//