LOCUS       Exported     3872 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3872
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3838
                     /label="Mfl042"
                     /note=""
                     /translation="MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIAKVLISEGQEIKVGDVVIEIDDGSATVEAAPAAEEENASVVGSTPVSNDLIPSRGPAPTQNVAAQPTPAPVATKHTDIEESFDVIVVGAGIGGYVSAIKTAQLGLKTLIIEKQYYGGVCLNVGCIPTKSLLRTAKVFEDIVHKAANLGIDMKTKDEPSINWNKALERKDGVVNKLTGGVKVLLTKNGVKQIIGEASALDKNTISVNGKKYHCDNLIIASGSVPNELPLPGFAEGRESGFLIDSTKILSLPKIPKTLTVIGGGVIGIEFGCLFAALGTKVTVIEGAPKILPMLDQDVTALMTKTLKEKYKIEIFTNAKVKEVKGKSVVFEIDGKEQTVKSDYCLESIGRKTVTKGFDGIGLELSERKSIIANDYGETNLEGVYAIGDVTSKIMLAHVASHAGIVTANRIALKANKPDAHDIKMDYSKIPSCIYSHPEIAMIGKTEQQLKEEGVEYKTFKFPFAAIGKALADDDTTGFVKIICEPKYKTLLGAHIIGNRATDMISEFTTLIECEGTITELARAIHPHPTMSEAIGEAAEALESGKSLNL"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATAGGAAC CTCTTACGCG GGGTCTCAAA TGTTTAAGGT
     2041 TAAATTCGCC GACATCGGTG AAGGTCTGAC CGAAGGTAAA GTGGCGGAAG TGCTGGTGAA
     2101 GCTGGGTCAG GAGATCAAAG AAGGTGATGC GCTGTTCTTC GTTGAAACCG ATAAAGTGAA
     2161 CAGCGAAATT CCGGCGCCGG TGGGCGGCAA AATCGCCAAA GTGCTGATCA GCGAAGGTCA
     2221 GGAAATCAAG GTGGGCGACG TCGTTATTGA GATCGACGAC GGTTCCGCTA CCGTGGAGGC
     2281 GGCACCAGCG GCCGAGGAAG AAAACGCTAG CGTGGTTGGC AGCACGCCAG TTAGCAACGA
     2341 CCTGATTCCG TCCCGTGGTC CAGCGCCGAC CCAAAATGTT GCCGCACAAC CGACCCCAGC
     2401 GCCTGTTGCG ACGAAGCACA CGGACATTGA GGAAAGCTTT GACGTTATCG TGGTCGGTGC
     2461 GGGTATTGGT GGCTATGTCT CCGCAATCAA GACCGCACAA TTGGGCCTCA AAACCCTTAT
     2521 TATCGAGAAA CAGTACTACG GCGGTGTCTG CTTGAACGTC GGCTGTATCC CGACCAAAAG
     2581 CCTGTTGCGT ACTGCGAAAG TCTTTGAGGA CATCGTTCAT AAAGCGGCAA ATCTGGGCAT
     2641 TGACATGAAA ACCAAGGATG AGCCGAGCAT CAATTGGAAT AAAGCTTTAG AACGCAAAGA
     2701 TGGTGTCGTT AACAAACTGA CTGGTGGCGT TAAAGTGTTG CTGACGAAAA ATGGTGTCAA
     2761 ACAGATCATC GGTGAAGCTA GCGCACTGGA CAAGAACACC ATTAGCGTTA ACGGTAAGAA
     2821 GTACCACTGC GACAATCTGA TTATCGCGAG CGGTAGCGTG CCGAACGAGC TGCCGCTGCC
     2881 GGGTTTCGCG GAGGGTCGTG AGAGCGGTTT CCTCATCGAT TCCACGAAAA TCCTGAGCCT
     2941 GCCGAAGATC CCGAAAACGC TGACCGTTAT TGGTGGCGGT GTTATCGGCA TTGAGTTTGG
     3001 TTGCCTGTTT GCCGCTTTGG GCACCAAAGT TACCGTTATC GAAGGTGCGC CGAAAATTCT
     3061 GCCGATGTTG GATCAGGATG TGACCGCATT GATGACCAAG ACGCTGAAGG AAAAGTACAA
     3121 GATTGAGATT TTCACCAATG CAAAGGTCAA AGAGGTCAAG GGCAAGAGCG TTGTGTTTGA
     3181 GATTGATGGC AAAGAGCAAA CCGTTAAATC TGACTATTGT CTTGAAAGCA TCGGCCGTAA
     3241 AACTGTGACG AAAGGTTTCG ACGGCATCGG TCTGGAGCTG AGCGAACGTA AAAGCATTAT
     3301 CGCCAATGAC TACGGCGAAA CCAATTTGGA AGGCGTTTAC GCAATTGGTG ACGTTACCAG
     3361 CAAAATCATG CTGGCACACG TGGCAAGCCA TGCTGGCATC GTTACCGCGA ATCGTATTGC
     3421 ACTGAAAGCA AACAAACCGG ACGCACATGA CATCAAAATG GATTACAGCA AGATCCCGAG
     3481 CTGTATTTAT AGCCACCCGG AGATTGCCAT GATTGGCAAG ACCGAACAAC AGCTGAAAGA
     3541 GGAAGGTGTG GAATATAAAA CCTTCAAGTT TCCGTTTGCG GCAATCGGCA AGGCCTTGGC
     3601 TGACGACGAC ACGACGGGCT TCGTCAAGAT CATTTGTGAA CCGAAGTATA AGACCCTGCT
     3661 CGGTGCCCAC ATCATCGGTA ACCGCGCGAC GGACATGATT AGCGAATTCA CGACCCTGAT
     3721 TGAGTGTGAG GGTACGATTA CCGAACTGGC TCGCGCTATC CACCCGCACC CGACCATGTC
     3781 CGAAGCGATT GGCGAAGCAG CCGAGGCCTT GGAAAGCGGT AAGTCATTGA ATCTGTGAAG
     3841 AGCTTAGAGA CCGCCCGGCA ATTCTTATAG CT
//