LOCUS       Exported     3016 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3016
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1054..1101
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2949..2965
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2966..2997
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..982
                     /translation="MNTLNEKRTILIIDGYHLLHKGYYGTLKRKKLATNREGVPINAIYTFVAKINQLVKKNNYYSIIVTLDMDEGCWRRELYPEYKAKRKETPEDLVPQKQIIREFLTAANIPWYEMPRYEADDIIGTINRIALKLDYDVHILSNDKDIFQLVGERTTVITNSSKDDEHILIDHEKVVEKFGCHPSQVADIKALMGDPSDNIKGVRYLHYAQAIDLLNKYGNIDNIFEHIDEINKNISKRLNESKELILTNKKITTIQDRLPIGRVNLKPIRVNWYGLSKFLKKQKMWAYVADVEKMASESRNKNSKVNKIKNEHISKEK"
                     /label="Mfl055"
                     /note=""
     primer_bind     complement(1106..1122)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1189..1777)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1968..2828)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2829..2933)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GAATACGCTG AATGAGAAGC GTACCATTCT
       61 GATCATTGAC GGTTATCATC TGCTGCACAA GGGCTACTAC GGTACCCTGA AACGCAAAAA
      121 ACTGGCAACG AACCGTGAGG GTGTTCCAAT TAACGCTATT TACACTTTCG TGGCGAAGAT
      181 CAATCAACTG GTTAAGAAAA ACAATTACTA TAGCATCATT GTGACCTTGG ATATGGATGA
      241 GGGTTGCTGG CGTCGTGAAC TGTATCCTGA GTATAAGGCG AAGCGTAAGG AAACGCCGGA
      301 GGACCTGGTT CCGCAAAAGC AAATCATTCG CGAGTTTCTG ACTGCTGCGA ACATCCCTTG
      361 GTATGAGATG CCTCGTTATG AAGCGGACGA TATCATCGGC ACCATCAATC GCATTGCGCT
      421 GAAGCTGGAC TATGACGTGC ACATTTTAAG CAATGATAAG GACATTTTCC AACTGGTGGG
      481 TGAGCGTACG ACGGTTATCA CGAACTCTAG CAAGGACGAC GAGCACATCC TGATCGATCA
      541 CGAAAAGGTG GTTGAGAAGT TTGGTTGTCA CCCATCTCAG GTTGCTGATA TCAAAGCACT
      601 GATGGGTGAT CCATCTGACA ACATTAAAGG CGTTCGTTAT CTGCACTACG CACAGGCAAT
      661 CGACCTGTTG AATAAGTATG GTAATATTGA CAATATTTTT GAGCACATCG ACGAGATCAA
      721 TAAAAATATT AGCAAACGTT TGAATGAAAG CAAGGAGCTG ATCCTGACCA ACAAAAAGAT
      781 TACCACGATT CAGGATCGTC TGCCGATCGG CCGCGTGAAC CTGAAACCAA TTCGTGTGAA
      841 CTGGTATGGT CTGTCTAAGT TCCTGAAAAA ACAAAAAATG TGGGCTTACG TTGCAGATGT
      901 CGAGAAGATG GCGTCTGAGA GCCGCAACAA GAATAGCAAA GTCAATAAGA TCAAGAATGA
      961 GCATATTAGC AAGGAGAAGT GAAGAGCTTA GAGACCCGAC TGCCGACATG ATCTATCGCT
     1021 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1081 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1141 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1201 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1261 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1321 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1381 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1441 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1501 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1561 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1621 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1681 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1741 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1801 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1861 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     1921 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     1981 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2041 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2101 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2161 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2221 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2281 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2341 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2401 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2461 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2521 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2581 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2641 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2701 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2761 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2821 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     2881 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     2941 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3001 TCAGGTGGAG TGGGAG
//