LOCUS       Exported     3911 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3911
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3877
                     /label="Mfl058"
                     /note="The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage."
                     /translation="MANIIKEINKPTLVLAHNKTLAMQLYYELMEFFPNNRVEYFVSNFDFFQPEAYKPATDLYINKDARINMELDMLRMSAMNALSISNDTIVVASVAAIYPSQDPVQYASSFFELKTGQKFSKRELLTYLVKTGYTRNDIENSPGTFSVKGDVIKVVPGWSLSAMYRFSLFDDHIEMIDMLNVVTGALIERISTVTIYPAQAYVTPEDKLKQACLNIREELAVRVKELKEEGKLLEAERLDQRTRYDLESLEEFGFCSGIENYSAHLDFRAPGETPYTLLDYFKGDFLTIIDESHIMVSQVRGMYNTDRSRKQTLVEYGFRLPSALDNRPLNFEEFTGKLKQVIYTSATPGDYELELVNHEVVEQIIRPTGLLDPVIEVKKTEGQIEDIIEQIHERKKVNEKVFITTLTIRMSEDLTSYLQERNIKVAYLHSELKTLERSDILNDLRRGVYDAVVGVNLLREGLDLPEVSLVCVLDADKQGFLRNTKSLIQTAGRAARNANGKVIFYADTISTSMQEAMDETNRRREIQQAYNKEHNITPVTITKKINQSTLSEATKKELEKIKKQKTAKGKKEAYQKTIDDIRAEMIQAAKDLDFEKAAVLRDTIIELEAKKSEVK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGAATTAC TGACCCCTCG GGGTCTCAAA TGGCCAATAT
     2041 TATCAAAGAG ATCAACAAGC CGACCCTGGT TCTGGCGCAC AACAAAACGC TGGCAATGCA
     2101 ACTGTACTAT GAGCTGATGG AATTTTTTCC TAATAACCGC GTCGAGTATT TTGTGAGCAA
     2161 CTTTGACTTT TTTCAGCCGG AAGCCTATAA ACCGGCAACG GACTTGTACA TTAACAAAGA
     2221 TGCACGTATC AATATGGAAC TGGATATGCT GCGTATGAGC GCAATGAACG CGTTGTCAAT
     2281 TTCGAACGAT ACGATCGTTG TTGCAAGCGT CGCGGCAATC TACCCGTCGC AGGATCCGGT
     2341 GCAGTATGCG AGCAGCTTTT TTGAGCTCAA GACCGGCCAG AAGTTTTCTA AGCGTGAACT
     2401 GCTGACCTAC CTGGTGAAAA CCGGCTACAC TCGTAACGAC ATTGAGAATA GCCCGGGTAC
     2461 GTTTTCTGTT AAGGGTGATG TGATTAAGGT TGTTCCGGGT TGGAGCCTGA GCGCAATGTA
     2521 TCGCTTTTCC CTTTTCGACG ATCATATTGA AATGATTGAC ATGCTCAATG TCGTCACCGG
     2581 TGCGCTGATC GAGCGCATCT CTACCGTTAC TATTTACCCG GCACAGGCTT ATGTGACCCC
     2641 GGAAGATAAA CTGAAACAGG CATGCCTGAA CATTCGTGAG GAACTCGCAG TGCGCGTCAA
     2701 GGAACTGAAA GAAGAAGGCA AACTGCTGGA GGCGGAGCGC CTGGATCAGC GTACGCGCTA
     2761 TGACTTGGAG AGCCTGGAGG AGTTTGGTTT CTGTAGCGGT ATCGAAAACT ACAGCGCCCA
     2821 TCTGGACTTC CGCGCACCGG GCGAAACCCC GTACACCCTG CTGGATTATT TCAAGGGTGA
     2881 TTTCCTGACC ATCATTGACG AAAGCCACAT CATGGTGAGC CAGGTCCGCG GCATGTACAA
     2941 CACGGACCGC TCACGTAAAC AAACCCTGGT TGAGTACGGC TTCCGTCTGC CGAGCGCACT
     3001 GGACAACCGT CCGCTGAACT TTGAGGAGTT CACCGGTAAA CTGAAACAGG TCATCTATAC
     3061 CAGCGCGACC CCGGGTGACT ACGAACTGGA GCTGGTCAAT CATGAGGTCG TTGAGCAAAT
     3121 CATTCGTCCG ACCGGTCTGC TGGACCCGGT CATTGAGGTC AAGAAAACTG AGGGTCAGAT
     3181 CGAGGATATT ATTGAGCAAA TCCATGAGCG TAAAAAAGTG AATGAGAAAG TGTTTATTAC
     3241 CACCTTGACG ATTCGTATGA GCGAAGATCT GACGTCTTAT CTGCAGGAGC GTAATATCAA
     3301 GGTCGCGTAT CTGCACAGCG AGTTAAAGAC CCTGGAGCGT TCTGACATTT TAAATGATCT
     3361 GCGCCGCGGT GTTTACGATG CGGTGGTGGG TGTCAACTTG CTGCGCGAAG GCCTGGATCT
     3421 GCCGGAGGTC AGCCTGGTCT GCGTGCTGGA CGCTGATAAA CAAGGCTTTC TGCGTAACAC
     3481 CAAGAGCCTT ATCCAAACCG CGGGTCGTGC GGCGCGTAAT GCCAATGGTA AGGTGATCTT
     3541 CTACGCAGAT ACGATCTCAA CCAGCATGCA AGAAGCAATG GACGAAACGA ATCGCCGTCG
     3601 TGAAATTCAA CAAGCTTATA ACAAAGAGCA CAACATTACC CCGGTTACGA TTACCAAAAA
     3661 GATCAACCAG TCTACCCTGT CTGAGGCGAC CAAGAAAGAG TTGGAAAAGA TCAAAAAACA
     3721 AAAGACGGCC AAGGGCAAAA AGGAGGCGTA CCAAAAGACG ATCGACGATA TTCGTGCCGA
     3781 AATGATTCAG GCGGCCAAAG ACCTGGACTT CGAAAAAGCA GCGGTCCTCC GTGATACCAT
     3841 CATTGAACTG GAGGCGAAGA AAAGCGAGGT GAAGTGAAGA GCTTAGAGAC CGTTACACCG
     3901 GGATCATAGC T
//