LOCUS       Exported     4913 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4913
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4879
                     /label="Mfl059"
                     /note="The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate."
                     /translation="MENKIVIKGARENNLKNVDLEIPKNKLVVFTGLSGSGKSSLAFATIYAEGRRRYIESLSAYARQFLGGNEKPDVDSIEGLSPAISIDQKTTSHNPRSTVGTVTEIYDYLRLLYARVGTPYCINGHGQIHAATIKEIINNIKSTTEDNEQVYVLSPVVRDKKGTHKDLLAKLLGEGFIRVSINGEIKMLEEEIILDKNQRHNIDIVVDRLIYHNDDEVHSRLFSAIEIALQYSNSLVKIKYPETENKEDNLFSNSYSCNVCGFNIPELEPRLFSFNAPLGACGDCDGLGVRLVSDPELIIPDKSLSINQGGVVYFKNFLNTDNLEWQKFKILCDYYYIDMNLPIDQLTNKQIKAILRGSEEQIEIKLLSSSGRRYDSYDFVEGVADLIQRRYFETKSEEIRKWYTKFMSSSVCQSCDGARLNPTALSIKIADKNIFDFTKMSIQDELEFILNVELTQTQAQIANLVLTEITNRISFLNEVGLGYLDLARTATTLSGGEAQRIRLAKQIGSQLTGILYVLDEPSIGLHQRDNDKLIKTLKHLRDLGNTLIVVEHDEDTMKTADWIVDVGPQAGEHGGQITFSGTYDEILKSDTITGRYLSGKESIPVPKKRRSGNGQKIDIVGARENNLKNVNVSIPLNKFIAITGVSGSGKSTLLEDIIYKGIRKNLARETIVTGKFSKMNGLENIDKVIFISQEPIGKTPRSNPATYTGVFDDIRDLFTNLPEAKIRGYKKGRFSFNVPGGRCEHCFGDGVITISMQFMPSVEVVCEICEGKRYNDETLQVKFKEKTIADVLNMTVETAAAFFENIPSIKDKLDTILEVGLGYIRLGQSATTLSGGEAQRIKLSTYLLKKQTGKTMFLLDEPTTGLHIDDVKRLVHVLNKLVDLGNTVVAIEHNLDFIKVADHVIDLGPEGGIGGGLIVAAGTPEQVADTKGSYTGTYLKEYLYNDFSR"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCAAA TGGAAAATAA
     2041 AATTGTTATC AAAGGCGCAC GCGAAAATAA TCTGAAGAAC GTAGATCTGG AAATTCCGAA
     2101 GAACAAATTG GTCGTTTTCA CCGGTCTGAG CGGCTCCGGC AAAAGCTCGC TGGCTTTTGC
     2161 CACGATTTAC GCGGAGGGTC GTCGTCGCTA TATCGAAAGC CTGTCGGCAT ACGCCCGCCA
     2221 GTTCCTGGGT GGCAACGAGA AGCCGGACGT GGACAGCATC GAGGGTTTGA GCCCGGCAAT
     2281 TAGCATTGAT CAAAAAACCA CCAGCCACAA CCCGCGTAGC ACCGTGGGTA CCGTTACGGA
     2341 GATCTACGAT TATCTGCGTC TGCTGTATGC ACGTGTTGGC ACCCCATATT GTATCAATGG
     2401 TCATGGTCAG ATCCATGCGG CGACGATCAA AGAGATTATC AACAACATCA AATCCACGAC
     2461 TGAGGACAAC GAGCAAGTTT ACGTTCTGTC TCCGGTGGTT CGTGATAAGA AAGGTACCCA
     2521 TAAGGATCTG CTGGCGAAAT TACTGGGTGA GGGCTTCATT CGTGTGTCCA TCAACGGCGA
     2581 GATCAAGATG CTGGAAGAGG AGATTATCCT GGACAAAAAC CAGCGCCACA ATATCGATAT
     2641 TGTTGTTGAC CGTCTGATTT ATCACAACGA TGATGAGGTG CATAGCCGCC TGTTCAGCGC
     2701 GATCGAAATT GCACTGCAAT ATAGCAACTC TCTGGTTAAA ATTAAGTACC CGGAAACCGA
     2761 GAACAAAGAG GATAATCTGT TCAGCAATAG CTACTCTTGT AATGTCTGCG GTTTTAACAT
     2821 CCCTGAGTTA GAGCCGCGTT TGTTCAGCTT TAACGCCCCG CTGGGTGCGT GTGGTGACTG
     2881 TGACGGTCTG GGCGTTCGTC TGGTAAGCGA CCCAGAGCTG ATTATCCCGG ATAAAAGCTT
     2941 GTCTATCAAC CAAGGTGGTG TTGTTTACTT CAAGAACTTC CTGAATACTG ACAATCTGGA
     3001 GTGGCAGAAG TTTAAAATCT TGTGCGACTA TTATTACATT GACATGAATC TGCCGATTGA
     3061 CCAGCTGACC AATAAGCAGA TTAAGGCTAT TCTGCGCGGC AGCGAGGAGC AGATTGAAAT
     3121 TAAGCTGTTA AGCAGCAGCG GCCGCCGTTA CGACTCTTAT GATTTTGTGG AGGGCGTTGC
     3181 CGATCTGATC CAACGCCGCT ACTTCGAAAC CAAGAGCGAG GAAATCCGTA AATGGTACAC
     3241 CAAATTTATG TCTAGCAGCG TGTGCCAATC TTGTGATGGT GCACGTCTGA ATCCGACCGC
     3301 TCTGAGCATT AAGATCGCCG ACAAAAATAT TTTCGATTTC ACGAAAATGA GCATTCAAGA
     3361 CGAGTTGGAG TTTATTTTGA ATGTGGAGCT GACCCAGACC CAAGCACAAA TTGCCAATCT
     3421 GGTTCTGACG GAAATTACCA ACCGTATCAG CTTTCTGAAC GAGGTCGGTT TGGGTTACCT
     3481 GGATTTGGCA CGCACCGCGA CCACTCTGTC TGGTGGCGAG GCACAGCGTA TTCGCCTGGC
     3541 AAAACAGATT GGCAGCCAGC TGACCGGCAT TCTGTACGTC CTGGACGAAC CGAGCATTGG
     3601 CCTCCACCAG CGTGACAACG ACAAGCTGAT TAAGACCCTG AAGCATCTGC GCGACCTGGG
     3661 TAATACCCTG ATTGTGGTTG AACACGATGA GGATACCATG AAAACCGCCG ACTGGATTGT
     3721 CGACGTTGGC CCGCAAGCGG GCGAACACGG CGGTCAGATT ACGTTCAGCG GTACCTACGA
     3781 CGAGATCTTG AAGTCCGACA CCATTACGGG CCGTTACCTG AGCGGTAAAG AGAGCATTCC
     3841 GGTGCCGAAG AAGCGTCGCT CCGGTAATGG CCAAAAAATC GATATCGTGG GCGCACGCGA
     3901 GAATAACCTG AAAAACGTCA ATGTGTCAAT CCCGCTGAAC AAATTTATCG CAATCACCGG
     3961 TGTTTCAGGT AGCGGCAAGA GCACTCTGCT GGAGGACATT ATTTACAAAG GTATCCGTAA
     4021 GAACCTGGCG CGCGAAACCA TTGTTACGGG CAAGTTTAGC AAAATGAACG GTCTGGAGAA
     4081 CATTGACAAG GTCATTTTTA TCAGCCAGGA ACCGATCGGT AAAACCCCTC GCAGCAACCC
     4141 GGCAACGTAC ACCGGTGTTT TTGACGATAT CCGTGATCTG TTTACCAATC TGCCGGAAGC
     4201 GAAAATCCGT GGTTACAAAA AAGGTCGCTT CAGCTTTAAT GTTCCGGGTG GTCGTTGCGA
     4261 GCACTGCTTT GGTGATGGTG TCATTACCAT TAGCATGCAG TTCATGCCGA GCGTCGAAGT
     4321 CGTCTGTGAA ATTTGCGAGG GCAAACGTTA CAATGACGAA ACGTTGCAAG TCAAGTTCAA
     4381 AGAAAAAACG ATCGCCGACG TGTTGAACAT GACTGTTGAA ACCGCAGCGG CGTTCTTCGA
     4441 AAACATTCCG AGCATTAAGG ACAAGCTGGA CACCATTCTG GAGGTCGGTC TGGGTTACAT
     4501 TCGTCTGGGT CAGTCCGCCA CCACGCTGAG CGGTGGTGAG GCGCAGCGTA TCAAACTGAG
     4561 CACGTACCTT CTGAAGAAAC AAACCGGTAA GACCATGTTC CTGTTGGACG AGCCGACCAC
     4621 TGGTCTGCAC ATCGACGATG TGAAACGTTT AGTTCACGTG CTGAACAAAC TGGTGGACCT
     4681 GGGTAACACC GTCGTCGCGA TCGAGCATAA TCTGGATTTC ATTAAAGTGG CCGACCACGT
     4741 TATTGACCTG GGCCCGGAGG GTGGTATTGG TGGCGGTCTG ATCGTGGCTG CGGGTACGCC
     4801 AGAGCAAGTT GCAGACACGA AAGGCAGCTA CACCGGTACC TATCTGAAAG AGTACCTGTA
     4861 CAACGACTTC TCTCGCTGAA GAGCTTAGAG ACCCCAGTAT CGGCAGCTAA TCT
//