LOCUS       Exported     3413 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3413
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2030..3379
                     /label="Mfl072"
                     /note=""
                     /translation="MITINTNKLEYKLIAVPNNETNINILDKPSCVTFISESKTFYLVIKKDSMSYLRQLKLGLASFIQMCKGDTNIDFNSFVKVIPNVNKEIIFNVICDVILFETHKFITYKKDVKTNDYLFNIDQSEDFSDLLDENILKNKYVNYARDLQDFPPNIGTSDFYGEKLLKDSSKIEGLKVSVLKNKELKELDMNLVLAVNQGSNHDANVVVMEFNNNPGGKKIALVGKGICFDTGGYDLKPSAFIEGMKFDMTGAAIVMSIAMALAEAKAKINLVAVGLFTDNKIGQKAVLPESIIKSKNGLTVEISNTDAEGRLVLADGITYAIREKNADVIYDIATLTGATAMALGESASGVFTEFDEMWKKLNEASNYTTERFWRLPIFAEHKEQVKNDSVLADLTNSCKKGPGGSTAAAFLTFFAEDKEFLHIDCSRVVRVGLKGQGYIVKTMFELLKK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC GGGTCTCAAA TGATTACGAT
     2041 CAACACTAAC AAGCTGGAGT ACAAACTGAT TGCTGTCCCG AATAACGAAA CGAACATTAA
     2101 CATTCTGGAT AAGCCAAGCT GCGTCACCTT CATTAGCGAG AGCAAGACGT TTTACCTGGT
     2161 GATTAAGAAG GACAGCATGA GCTATCTGCG CCAACTGAAA CTGGGCCTGG CGTCCTTCAT
     2221 CCAAATGTGC AAGGGTGATA CCAATATTGA TTTTAATTCC TTTGTAAAGG TCATCCCAAA
     2281 TGTTAACAAA GAAATTATTT TCAACGTCAT TTGTGATGTG ATTTTGTTTG AAACCCATAA
     2341 GTTCATCACG TACAAGAAAG ATGTTAAAAC CAACGATTAC CTGTTTAACA TCGATCAATC
     2401 CGAGGATTTT AGCGACTTGC TGGACGAAAA TATCCTGAAG AATAAGTATG TGAATTATGC
     2461 GCGCGATCTG CAGGATTTCC CTCCGAATAT CGGCACGAGC GATTTTTACG GTGAAAAACT
     2521 GCTGAAAGAC AGCAGCAAGA TTGAGGGCCT GAAAGTGAGC GTTCTGAAAA ATAAAGAACT
     2581 GAAAGAGTTG GACATGAACC TTGTCCTGGC GGTGAATCAA GGTAGCAACC ACGATGCGAA
     2641 CGTTGTCGTT ATGGAATTCA ACAACAACCC TGGTGGTAAG AAAATCGCCC TGGTTGGTAA
     2701 AGGTATTTGC TTCGACACCG GTGGTTACGA CCTGAAGCCG AGCGCCTTCA TCGAAGGTAT
     2761 GAAATTCGAC ATGACGGGTG CAGCAATTGT TATGAGCATT GCCATGGCTC TGGCGGAGGC
     2821 CAAGGCTAAG ATCAACCTGG TGGCTGTTGG TCTGTTTACC GATAATAAAA TCGGTCAAAA
     2881 GGCGGTACTG CCGGAGAGCA TTATTAAAAG CAAAAATGGT CTGACCGTGG AGATTAGCAA
     2941 CACTGACGCT GAGGGCCGCC TGGTACTCGC AGATGGTATC ACCTATGCTA TTCGTGAGAA
     3001 AAACGCCGAT GTCATCTATG ATATTGCGAC GTTGACGGGT GCGACCGCCA TGGCGCTGGG
     3061 TGAAAGCGCG TCGGGTGTTT TCACCGAGTT TGACGAGATG TGGAAAAAGC TGAACGAGGC
     3121 ATCCAATTAC ACGACCGAGC GTTTTTGGCG TCTGCCGATC TTTGCAGAGC ATAAAGAACA
     3181 AGTTAAGAAT GATTCTGTCC TGGCCGACCT GACCAACAGC TGCAAAAAAG GCCCAGGTGG
     3241 TAGCACCGCA GCGGCGTTCT TGACGTTTTT TGCAGAAGAT AAAGAGTTCC TGCACATCGA
     3301 TTGTTCCCGC GTTGTCCGTG TTGGTCTGAA GGGCCAGGGT TATATTGTCA AAACCATGTT
     3361 CGAGCTGCTG AAGAAGTGAA GAGCTTAGAG ACCCGGTGCT TTTGCGTGAT ATT
//