LOCUS       Exported     2986 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2986
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1024..1071
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2919..2935
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2936..2967
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..952
                     /label="Mfl078"
                     /note=""
                     /translation="MKYYNYQSKYNNSTIKLKWIYSFLNYNLFLGNLCHIFLFQIKEWLKRQYKFLFFQYIIRSMKSNKENYYGNLSSLVYNQSKPPGTSIDGDIEFYTNELIKRDGLVLEAGVGNGRMAIPLLRKGISIFGLDNSKEMLAIYEENLKKYNMESQLILGDLINFETDLKFETIIMPNGSFCLLNRNDIYSVLSNFKRHLTENGKIYLDLIFPTSFKPGNIHEYDFILDNNDTVKIKNKSISIDWLLQKTYTELEYLNNSEIEIQPFSLWWYGVEEFKSILEKLEFKNIKTIKNYNNIKNLNLKTLTFVFEK"
     primer_bind     complement(1076..1092)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1159..1747)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1938..2798)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2799..2903)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GAAATATTAT AACTATCAAA GCAAATATAA
       61 CAATAGCACC ATCAAGCTGA AGTGGATTTA CAGCTTCTTG AATTACAATC TGTTCCTCGG
      121 CAATCTGTGC CACATCTTCC TGTTTCAGAT CAAGGAGTGG CTGAAACGTC AGTACAAGTT
      181 TTTGTTTTTT CAGTACATCA TTCGTTCGAT GAAAAGCAAT AAAGAGAATT ACTACGGTAA
      241 TCTGAGCTCC CTGGTTTACA ACCAAAGCAA ACCGCCGGGT ACGAGCATTG ATGGTGATAT
      301 TGAATTCTAC ACTAACGAGC TGATTAAACG TGACGGTCTG GTTCTGGAGG CGGGTGTGGG
      361 TAACGGTCGC ATGGCAATTC CGCTGCTGCG TAAGGGTATC AGCATTTTCG GCTTGGATAA
      421 TTCTAAAGAA ATGCTGGCAA TTTACGAAGA GAATCTGAAG AAATATAACA TGGAGAGCCA
      481 GCTGATCCTG GGTGACCTGA TTAACTTTGA AACCGATCTG AAATTTGAAA CGATTATCAT
      541 GCCGAACGGT AGCTTTTGCT TGCTGAATCG TAATGACATT TACTCAGTGC TGTCTAATTT
      601 CAAGCGTCAT TTGACTGAAA ACGGCAAAAT TTATCTGGAC CTGATCTTCC CGACCTCTTT
      661 CAAGCCTGGT AACATCCATG AATATGACTT CATTCTGGAC AATAACGACA CCGTCAAAAT
      721 TAAGAATAAG TCCATCAGCA TTGATTGGCT GCTGCAAAAG ACCTACACTG AGCTGGAGTA
      781 CCTGAACAAT AGCGAGATTG AAATTCAACC TTTCTCTCTG TGGTGGTACG GCGTAGAGGA
      841 GTTCAAGTCC ATTCTGGAGA AGCTGGAGTT CAAGAATATT AAAACCATTA AAAACTACAA
      901 TAATATTAAA AACCTTAATC TGAAAACCCT GACGTTCGTT TTCGAGAAGT GAAGAGCTTA
      961 GAGACCGCCA TTGGAAAACT GAACGTCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
     1021 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1081 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1141 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1201 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1261 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1321 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1381 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1441 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1501 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1561 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1621 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1681 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1741 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     1801 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     1861 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     1921 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     1981 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     2041 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     2101 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     2161 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     2221 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     2281 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     2341 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     2401 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     2461 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     2521 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     2581 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     2641 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     2701 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     2761 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     2821 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     2881 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     2941 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TCAGGTGGAG TGGGAG
//