LOCUS       Exported     3472 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3472
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1510..1557
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3405..3421
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3422..3453
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1438
                     /label="Mfl079"
                     /note=""
                     /translation="MNHNFKLKEKKIWIKSYWWALIILVGSLLITTFFDYQITDFFTQGMNNYFLRQIVNFVSSGGNFIITIPIGIIAATILETLYFKYKIKNNFIKLVPYTLLILGLIFFGSLYCIQKASYTFSDDIKNNTLNSIWIKTLTTWKEPIIICSIWIVLMTAILSYGTFFFRIKFASRNDILENKYWIGAFEMLTVFIISYFTVLVLKLFFARPFYFSVEYRNLFGMSDSNEIEHLFDGLTIENYVNHPGAKLLIDLYLQTEGLELNDNNFKLATNWMAETLWQIPYGPAPEPAWRWTYWFIPNIFSRVDSHTINDGVIYWSSQAFNGDFPSGHIEIPLSIFGTFFIIRRSGSVNFKNKKILLFTILTSIMFVLTFFFMIVYRFHWITDMIFTPILYFAFLPIAYFKTERWIYAIMFRFSKNKKILITNNGNKTEFKITVNQEYVIFKIKKKGKKAFKYEYKIKTKYPSLLVDRI"
     primer_bind     complement(1562..1578)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1645..2233)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2424..3284)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3285..3389)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GAACCACAAC TTCAAACTGA AAGAGAAGAA
       61 AATTTGGATT AAAAGCTATT GGTGGGCCCT GATTATCTTG GTTGGTTCGT TGCTGATCAC
      121 GACCTTTTTT GACTATCAAA TTACCGATTT TTTCACGCAG GGCATGAATA ACTACTTCTT
      181 GCGTCAAATC GTCAATTTCG TTTCTAGCGG TGGCAATTTC ATCATTACGA TTCCGATCGG
      241 CATTATTGCG GCTACGATCC TGGAAACGTT GTACTTTAAG TACAAAATTA AGAACAACTT
      301 CATCAAACTG GTCCCGTATA CGCTGCTGAT CCTGGGTCTG ATCTTTTTCG GTAGCCTGTA
      361 TTGCATCCAG AAGGCCTCCT ATACGTTCAG CGACGACATC AAGAATAATA CGTTAAATAG
      421 CATCTGGATT AAGACCTTAA CGACGTGGAA AGAACCGATT ATCATTTGTT CTATCTGGAT
      481 CGTCCTGATG ACCGCTATCC TGAGCTATGG TACCTTCTTT TTCCGCATTA AGTTCGCCAG
      541 CCGTAATGAT ATTCTGGAGA ACAAATATTG GATTGGTGCG TTTGAAATGC TGACCGTGTT
      601 TATTATCAGC TACTTTACGG TCCTGGTTCT GAAACTGTTC TTCGCGCGTC CGTTCTACTT
      661 CTCCGTCGAA TACCGTAATC TGTTTGGCAT GAGCGATAGC AACGAGATTG AACACCTGTT
      721 TGATGGTCTG ACCATTGAGA ATTATGTTAA CCACCCGGGT GCAAAGCTGC TGATCGATCT
      781 GTATCTGCAG ACGGAAGGCC TGGAACTGAA TGACAATAAC TTCAAGCTGG CGACCAATTG
      841 GATGGCCGAA ACCCTGTGGC AAATCCCGTA CGGTCCGGCA CCGGAGCCGG CGTGGCGTTG
      901 GACGTATTGG TTTATCCCTA ACATCTTCAG CCGCGTGGAT TCTCATACGA TCAACGACGG
      961 CGTTATTTAT TGGAGCAGCC AGGCTTTTAA CGGCGATTTC CCGAGCGGTC ATATTGAGAT
     1021 CCCGTTGAGC ATCTTTGGTA CCTTCTTTAT CATTCGTCGT AGCGGCAGCG TGAATTTCAA
     1081 AAATAAAAAG ATTTTGTTGT TCACCATTTT GACGAGCATT ATGTTCGTTC TGACCTTCTT
     1141 TTTCATGATT GTGTATCGCT TTCACTGGAT CACGGACATG ATCTTCACGC CGATCCTGTA
     1201 CTTCGCCTTC TTGCCAATTG CGTATTTTAA AACTGAGCGC TGGATCTATG CCATTATGTT
     1261 CAGATTCTCT AAAAATAAGA AAATCCTGAT TACCAATAAC GGTAATAAGA CCGAGTTCAA
     1321 GATCACCGTC AACCAAGAAT ACGTCATCTT CAAAATTAAA AAGAAAGGCA AAAAAGCTTT
     1381 CAAATACGAG TACAAGATTA AGACGAAATA TCCGAGCCTG CTGGTGGACC GTATTTGAAG
     1441 AGCTTAGAGA CCGGTACGGT TTACAGACCA CTCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1501 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1561 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1621 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1681 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1741 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1801 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1861 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1921 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1981 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2041 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2101 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2161 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2221 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2281 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2341 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2401 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2461 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2521 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2581 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2641 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2701 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2761 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2821 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2881 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2941 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3001 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3061 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3121 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3181 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3241 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3301 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3361 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3421 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//