LOCUS       Exported     2935 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2935
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      973..1020
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2868..2884
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2885..2916
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..901
                     /translation="MLRKYEAMFILDQDTQDVNALSSRMIEIISKDGKVIEKNDLGLIEFAYKINHKKKGHYFVVIVEATAEAIKEFERIANIEKNVVRTLIINTENEQGYEQSVQLSKTDMTKFEEERKAKRDFKKPFVKKEFNKPTEKRTFEKPAEVTVEVKEVVVEEVKQTVKPAKKVAEAKVEEVSHEEAHDFVSKMEAKYKAHLAETVEEHVEEVEVEEAKTTAKKPAAPKMSAAERAKVDGSHNIDEERYELQKYSNKLRSVAIEKNLPKKLQEVNLRDLTKKELIEYMRKVRAALAK"
                     /label="rpsF"
                     /note="Binds together with S18 to 16S ribosomal RNA."
     primer_bind     complement(1025..1041)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1108..1696)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1887..2747)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2748..2852)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GCTGCGTAAG TATGAGGCAA TGTTTATCCT
       61 GGATCAAGAC ACCCAAGATG TCAATGCGCT GAGCAGCCGT ATGATCGAGA TTATTTCCAA
      121 GGATGGTAAA GTCATCGAGA AAAACGACCT GGGTTTAATC GAATTTGCGT ACAAGATTAA
      181 TCACAAGAAA AAGGGCCACT ACTTCGTTGT TATTGTGGAG GCTACGGCAG AAGCTATCAA
      241 AGAGTTCGAA CGTATTGCCA ATATTGAAAA GAATGTTGTT CGTACGCTGA TTATCAACAC
      301 CGAAAACGAG CAAGGTTACG AGCAGAGCGT TCAGCTGAGC AAAACCGATA TGACGAAATT
      361 CGAGGAAGAA CGCAAAGCAA AGCGTGATTT TAAGAAACCG TTCGTCAAAA AGGAGTTTAA
      421 TAAACCGACT GAAAAGAGAA CTTTCGAAAA GCCGGCGGAA GTCACGGTTG AGGTGAAGGA
      481 GGTTGTGGTC GAGGAAGTGA AACAGACCGT GAAGCCTGCG AAAAAAGTCG CGGAGGCGAA
      541 AGTCGAGGAG GTGAGCCATG AGGAGGCACA TGACTTTGTC AGCAAAATGG AAGCCAAGTA
      601 CAAAGCTCAC CTGGCGGAAA CCGTGGAGGA GCACGTTGAA GAGGTTGAGG TGGAAGAGGC
      661 AAAGACTACC GCGAAGAAGC CTGCGGCGCC GAAGATGAGC GCGGCGGAAC GTGCCAAGGT
      721 GGATGGCTCC CACAACATTG ATGAGGAGCG TTACGAGCTG CAGAAGTATT CGAATAAACT
      781 GCGCAGCGTG GCAATTGAGA AGAATCTGCC AAAGAAACTG CAGGAGGTCA ATCTGCGTGA
      841 TCTGACTAAG AAAGAGTTGA TTGAATATAT GCGCAAAGTG CGCGCGGCGC TGGCGAAGTG
      901 AAGAGCTTAG AGACCCCGAG GGGTCAGTAA TTCTTCGCTG AGGTGTCAAT CGTCGGAGCC
      961 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1021 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1081 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1141 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1201 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1261 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1321 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1381 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1441 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1501 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1561 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1621 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1681 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     1741 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     1801 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     1861 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     1921 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     1981 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     2041 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     2101 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2161 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2221 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2281 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2341 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2401 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2461 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2521 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2581 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     2641 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     2701 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     2761 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     2821 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     2881 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GGGAG
//